A Novel Multilayered Membrane for Periodontal Regeneration

用于牙周再生的新型多层膜

基本信息

批准号：
8692736
负责人：
Marco C Bottino
金额：
$ 14.31万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-01 至 2018-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8692736
关键词：
Adhesions Adult Affect Alkaline Phosphatase Alveolar Award Biocompatible Materials Biology Bone Density Bone Regeneration Bone Surface Bone Tissue Canis familiaris Cell Count Cell Culture Techniques Cell Survival Cellular biology Cementoblast Chronic Clinical Clinical Trials Connective Tissue Cues Defect Dental Dental Cementum Dentists Development Development Plans Disease Effectiveness Environment Epithelial Attachment Evaluation Extracellular Matrix Proteins FGF2 gene Fibroblast Growth Factor Fibroblast Growth Factor 2 Fibroblasts Foundations Funding Goals Gray unit of radiation dose Growth and Development function Guided Tissue Regeneration Height Histocompatibility Testing Human Image Implant In Vitro Inflammatory Knowledge Laboratories Lead Left Literature Location Mandible Measurement Measures Membrane Mentors Mesenchymal Stem Cells Methodology Methods Metric Modeling Molecular Biology Molecular Profiling Molecular and Cellular Biology Nanotechnology Natural regeneration Operative Surgical Procedures Oral Osteogenesis Patient Care Periodontal Ligament Periodontitis Polytetrafluoroethylene Positioning Attribute Principal Investigator Procedures Qualifying Research Resources Scientist Secure Side Signal Transduction Stem cells Supervision Surface Techniques Tissue Engineering Tissues Tooth Loss Tooth root structure Tooth structure Training Translating Translational Research aging population alveolar bone biomaterial development bone calcium phosphate career development clinical practice craniofacial design implantation in vitro Assay in vivo innovation migration mineralization multidisciplinary novel osteoblast differentiation programs public health relevance regenerative regenerative therapy research study response skeletal skills socioeconomics soft tissue tissue reconstruction tissue regeneration

项目摘要

DESCRIPTION (provided by applicant): With the US population aging, it is important to find novel dental biomaterials that will promote periodontal regeneration, and translate these to clinical practice. As both a dentist and materials scientist, the applicant is in a position to brige the gap between biomaterials development. and their application in patient care using nanotechnology, tissue engineering and clinical knowledge. The purpose of this award is to provide the principal investigator extensive training in cellular and molecular biology, skeletal biology, and translational research with an emphasis on periodontal tissue regeneration. The experiments in this application have been designed to incorporate new methodologies, and will be guided by a multidisciplinary team of experts who will provide the required training to advance Dr. Bottino's scientific technical and analytic skills. The principal investigator's ultimae goal is to become a productive and independent clinician-scientist who can secure federal funding to sponsor an independent research program. The central hypothesis of the proposed research is that a multilayer membrane capable of stimulating the regeneration of various tissue types in a location specific manner, through the addition of calcium phosphate and fibroblast growth factor-2 (CaP/FGF2) and FGF2, will lead to alveolar and periodontal tissue regeneration in the appropriate locations. The literature indicates that no single implantable biomaterial can consistently guide the coordinated growth and development of multiple tissue types, especially in very large periodontal defects. Thus, there is a critical need to develop regenerative therapies to promote oral and craniofacial tissue regeneration and reconstruction. The specific aims of the proposed research are (1) To use an in vitro assay model to maximize the tissue-specific regenerative capacity of the multilayered implant surface layers, (2) To assess the effectiveness of CaP/FGF2 on bone regeneration in an alveolar defect using an in vivo canine model, and (3) To assess the effectiveness of the multilayered implant to regenerate soft and mineralized tissues in a location-specific manner using an in vivo canine model. The principal investigator has devised a comprehensive career development plan that includes formal coursework, laboratory guidance to cover intensive cell and molecular biology training, as well as in vivo techniques combined with a diverse set of bone/periodontal tissue regeneration related analyses under the supervision of his mentoring team. Collectively, the K08 award has the capacity to provide the resources and training needed to give the applicant the foundation and critical theoretical and practical means to become a highly qualified clinician-scientist who can translate his knowledge of biomaterials to clinical practice.

描述（由申请人提供）：随着美国人口老龄化，寻找能够促进牙周再生的新型牙科生物材料并将其转化为临床实践非常重要。作为牙医和材料科学家，申请人有能力缩小生物材料开发之间的差距。以及利用纳米技术、组织工程和临床知识在患者护理中的应用。该奖项的目的是为主要研究者提供细胞和分子生物学、骨骼生物学和转化研究方面的广泛培训，重点是牙周组织再生。该应用程序中的实验旨在融入新的方法，并将由多学科专家团队指导，他们将提供所需的培训，以提高 Bottino 博士的科学技术和分析技能。首席研究员的最终目标是成为一名高效且独立的临床科学家，能够获得联邦资金来赞助独立研究项目。该研究的中心假设是，通过添加磷酸钙和成纤维细胞生长因子-2 (CaP/FGF2) 和 FGF2，能够以特定位置的方式刺激各种组织类型再生的多层膜将导致牙槽和牙周组织在适当的位置再生。文献表明，没有一种可植入生物材料能够一致地引导多种组织类型的协调生长和发育，特别是在非常大的牙周缺损中。因此，迫切需要开发再生疗法促进口腔和颅面部组织的再生和重建。拟议研究的具体目标是 (1) 使用体外测定模型最大限度地提高多层种植体表面层的组织特异性再生能力，(2) 评估 CaP/FGF2 对牙槽骨再生的有效性(3)使用体内犬模型评估多层植入物以特定位置的方式再生软组织和矿化组织的有效性。首席研究员制定了全面的职业发展计划，包括正式课程、涵盖密集细胞和分子生物学培训的实验室指导，以及在他的监督下结合各种骨/牙周组织再生相关分析的体内技术。辅导团队。总的来说，K08 奖项有能力提供所需的资源和培训，为申请人提供基础和关键的理论和实践手段，使其成为一名高素质的临床医生科学家，将其生物材料知识转化为临床实践。