Label-free, non-invasive and cost-effective monitoring of HIV viral load using a nano-plasmonic sensor on a contact lens

使用隐形眼镜上的纳米等离子体传感器对 HIV 病毒载量进行无标记、非侵入性且经济有效的监测

• 批准号: 9135168
• 负责人: Aydogan Ozcan
• 金额: $ 19.25万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9135168
• 关键词: Address; Adherence; Anti-Retroviral Agents; Artificial Tears; Biological; Blood; Car Phone; Caring; Cellular Phone; Chemicals; Chemistry; Communities; Contact Lenses; Deposition; Detection; Development; Devices; Diagnostic; Disease; Environment; Eye; Film; Future; Geometry; HIV; HIV Infections; Health; Hepatitis B Virus; Home environment; Human; Hydrophilic Contact Lenses; Label; Laboratories; Liquid substance; Masks; Measurement; Measures; Mechanics; Metals; Methods; Microscope; Microscopic; Microscopy; Monitor; Nanostructures; Optics; Patients; Performance; Preparation; Process; Property; Reader; Research; Resources; Retrieval; Risk; Safety; Sampling; Scheme; Simplexvirus; Source; Surface; Techniques; Technology; Testing; Time; Training; Viral Load result; Viral load measurement; Virus; Virus Diseases; Vision; Work; antiretroviral therapy; base; cost; cost effective; design; flexibility; high reward; high risk; imprint; improved; instrument; light weight; lithography; manufacturing process; nanoplasmonic; nanosensors; novel; operation; particle; plasmonics; public health relevance; response; sensor; spectrograph; tool; transmission process

 DESCRIPTION (provided by applicant: Assessment and monitoring of the viral load of HIV+ patients and their adherence to antiretroviral (ART) therapy are critically important for controllig the course of the disease, assessing transmission risks, and monitoring of HIV infection within communities. Viral load measurements are currently conducted in laboratory settings using costly and bulky apparatus including benchtop optical microscopes and sample preparation steps that require advanced lab instruments, which are hard to find and operate in resource limited environments or at the patient's home. In addition to the complexity, cost and bulkiness of existing tools, HIV viral load measurement methods currently rely on blood drawing, which can in general create significant sanitary and safety concerns in resource limited settings or at home. The proposed research plan covers the development and testing of a field-portable and label-free flexible sensing platform that is based on surface plasmon resonant (SPR) properties of specially designed quasi three-dimensional metal nanostructures (MNS) which will be integrated with commercially available soft contact lenses to create a cost effective and non-invasive diagnostic tool that can work even at home for measuring the viral load of HIV+ patients using tear. This new sensing platform will be designed to achieve flexibility by constructing its sensing unit on flexible substrates to enable intimate, direct contact with curved or irregular surfaces such as the human eye without any additional sample retrieval or processing steps and without obscuring natural vision or causing user discomfort. In addition to the flexible plasmonic sensor integrated onto the contact lens surface, we will also create a field portable and cost- effective smart phone based spectral reader platform to specifically analyze and quantify the viral load through multi-spectral imaging and automated analysis of the built-in nano-sensor on the surface of each contact lens. Previous studies have shown that HIV particles exist in tear and ocular fluids of HIV+ patients, and therefore tracking HIV status through tear can present a much safer and simpler approach for monitoring of HIV+ patients and their adherence to ART. In addition to improving care adherence and non- invasively quantifying the viral load of HIV+ patients through tear films, this proposed platform will ultimately provide a simple, cost-effective and light-weight toolset for multiplexed biological and chemical sensing needs, enabling various other future biomedical applications of wearable sensors.

 描述对于疾病的过程非常重要负载方法目前依赖于资源有限设置或在家中的安全性问题。专门设计的纳米结构（MN）将与商业上可用的软性隐形眼镜集成在一起，以创建一种昂贵和非侵入性工具，即使在家中可以用撕裂来测量HIV+患者的病毒载荷。在柔性基础上构建其传感单元，以实现与弯曲的直接接触 或不规则地表面huthout additional样品检索或处理步骤，并引起用户不适。 基于智能手机的便携式和成本效益的光谱读取器Al成像和对每个隐形眼镜表面的内置纳米传感器的自动分析。除了提高护理依从性和非侵入性通过撕裂膜量化HIV+患者的病毒载荷外，该支撑平台还提供了一个简单的尖端生物学和 化学传感需求，实现可穿戴传感器的其他各种生物医学应用。