Lensfree On-Chip Near-field Microscopy based on Resonant Nano-Apertures

基于共振纳米孔径的无透镜片上近场显微镜

• 批准号: 7574253
• 负责人: Aydogan Ozcan
• 金额: $ 17.45万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-30 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7574253
• 关键词: Algorithms; Area; Biology; Blood specimen; CD4 Positive T Lymphocytes; Cell Count; Cellular Phone; Chemistry; Computers; Condition; Count; Data; Developed Countries; Developing Countries; Development; Devices; Diagnostic; Electronics; Erythrocytes; Genomics; Health Personnel; Healthcare; Histopathology; Image; Imaging technology; Internet; Lighting; Measurement; Mechanics; Medical; Medicine; Metals; Microfluidic Microchips; Microfluidics; Microscope; Microscopy; Modality; Nanoarray Analytical Device; Optics; Patients; Physicians; Positioning Attribute; Property; Proteomics; Public Health; Purpose; Research; Resolution; Resources; Sampling; Scanning; Scheme; Speed; Standards of Weights and Measures; Surface; System; Telepathology; Testing; Time; Today; Training; Whole Blood; base; cell type; concept; cost; design; digital; high throughput screening; improved; interest; lens; miniaturize; nano; near field microscopy; novel; photonics; point of care; sensor; transmission process; two-dimensional

DESCRIPTION (provided by applicant): To achieve high spatial resolution, most optical microscopes rely on expensive components such as high numerical aperture objectives or other lenses. Due to such free-space components, most high resolution microscopes still remain bulky, making them harder to integrate with cost-effective and miniaturized systems. However, in medicine/biology there exists a set of applications that would highly benefit from miniaturized high resolution microscopes that are ideally on chip. For this purpose, the proposed research plan aims to develop a new form of lens-free, compact digital microscope using a densely packed array of on chip nano-apertures. By properly designing an array of metallic nano-apertures, a high spatial resolution will be achieved even without using any lens or mechanical scanning. For this end, the designed nano-array will be fabricated onto a standard opto-electronic sensor array, and the object of interest will be in direct contact with the aperture array. As a result, the proposed on chip microscope can image two dimensional objects (>5 mm2 wide) at a high spatial resolution of d500 nm, and with a fast imaging speed of ~150 mm2/min. Overall, this application aims to advance the current state of on chip microscopy by using the key concepts of nano-photonics and plasmonics. Because the proposed approach is lensfree, it can be integrated with microfluidic systems in a compact space, and thus can significantly improve the way that high-throughput screening microscopy or point-of-care diagnostics are currently done. High resolution and rapid imaging technologies that can be integrated with disposable microfluidic devices are urgently needed today, especially for the developing world, where the resources are scarce. Another significant impact of the proposed imaging modality in health care may be in the field of histopathology. The proposed near-field imaging scheme can potentially enable quite rapid capturing of high resolution images of histopathology samples, which can then be transmitted over the internet to any physician in the world. This opportunity, which is termed "telepathology", will imply the global sharing of medical resources. For this end, this application may enable practical implementations of telepathology by significantly improving the imaging speed (>150 mm2/min). This rapid image acquisition speed may also have a significant impact on micro-array based genomics or proteomics, where massive amounts of high resolution data need to be collected in a short amount of time. PUBLIC HEALTH RELEVANCE: Lensfree On-Chip Near-field Microscopy based on Resonant Nano-Apertures Project Narrative We propose to develop a novel high resolution and lens-free digital microscope that is based on optical resonance properties of a specially designed array of nano-apertures fabricated on an opto-electronic sensor chip. This new microscopy approach does not require any mechanical scanning or objective-lenses, and therefore offers a simpler and more compact approach for high resolution near-field imaging. Furthermore, the image acquisition and computation time is fairly short, making it a quite fast imaging modality that can significantly improve the way that high-throughput screening microscopy or point-of-care diagnostics are currently done.

描述（由申请人提供）：为了实现高空间分辨率，大多数光学显微镜都依赖昂贵的组件，例如高数值孔径目标或其他镜头。由于如此自由空间的组件，大多数高分辨率显微镜仍然保持笨重，因此很难与成本效益和微型化系统集成。但是，在医学/生物学中，存在一系列应用，这些应用将从理想情况下是芯片上的微型高分辨率显微镜中受益匪浅。为此，拟议的研究计划旨在使用密集的芯片纳米施加阵列开发一种新形式的无镜头，紧凑的数字显微镜。通过正确设计一系列金属纳米孔，即使不使用任何镜头或机械扫描，也将达到高空间分辨率。为此，设计的纳米阵列将被制造在标准的光电传感器阵列上，并且感兴趣的对象将直接与孔径阵列接触。结果，芯片显微镜上的提议可以在D500 nm的高空间分辨率下成像二维对象（> 5 mm2宽），并且快速成像速度约为150 mm2/min。总体而言，该应用程序旨在通过使用纳米 - 光子学和等离子体学的关键概念来提高芯片显微镜的当前状态。由于所提出的方法是无镜头的，因此可以与紧凑型空间中的微流体系统集成，因此可以显着改善当前完成高通量筛选显微镜或护理点诊断的方式。当今急需将可以与一次性微流体设备集成的高分辨率和快速成像技术如今需要急切需要，尤其是对于资源稀缺的发展中国家而言。拟议的成像方式在医疗保健中的另一个重要影响可能是组织病理学领域。提出的近场成像方案可以有可能快速捕获组织病理学样本的高分辨率图像，然后可以通过互联网传播给世界上任何医生。这个被称为“心脏病学”的机会将暗示全球医疗资源的共享。对于此目的，该应用程序可以通过显着提高成像速度（> 150 mm2/min）来实现远程病理学的实际实现。这种快速的图像采集速度也可能对基于微阵列的基因组学或蛋白质组学产生重大影响，其中需要在短时间内收集大量的高分辨率数据。 公共卫生相关性：基于谐振纳米孔径叙事的晶状体片上近场显微镜，我们建议开发一种新颖的高分辨率和无镜头的无镜头数字显微镜，该数字显微镜基于特殊设计的一系列纳米孔径的光学共振属性在光电传感器芯片上制造。这种新的显微镜方法不需要任何机械扫描或客观镜头，因此提供了更简单，更紧凑的方法，用于高分辨率近距离成像。此外，图像采集和计算时间相当短，使其成为一种非常快速的成像方式，可以显着改善目前完成高通量筛选显微镜或护理点诊断的方式。