Label-free, non-invasive and cost-effective monitoring of HIV viral load using a nano-plasmonic sensor on a contact lens

使用隐形眼镜上的纳米等离子体传感器对 HIV 病毒载量进行无标记、非侵入性且经济有效的监测

• 批准号: 9295029
• 负责人: Aydogan Ozcan
• 金额: $ 23.1万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2019-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9295029
• 关键词: Address; Adherence; Anti-Retroviral Agents; Artificial Tears; Biological; Biosensing Techniques; Biosensor; Blood; Car Phone; Caring; Cellular Phone; Chemicals; Chemistry; Communities; Contact Lenses; Deposition; Detection; Development; Devices; Dimensions; Disease; Environment; Eye; Film; Future; Geometry; HIV; HIV Infections; Health; Hepatitis B; Home environment; Human; Hydrophilic Contact Lenses; Label; Laboratories; Liquid substance; Masks; Measurement; Measures; Mechanics; Metals; Methods; Microscope; Microscopic; Microscopy; Monitor; Nanostructures; Optics; Patients; Performance; Periodicity; Preparation; Property; Reader; Research; Resources; Retrieval; Risk; Safety; Sampling; Scheme; Simplexvirus; Source; Surface; Techniques; Technology; Testing; Time; Training; Viral Load result; Viral load measurement; Virus; Virus Diseases; Vision; Work; antiretroviral therapy; base; cost; cost effective; design; flexibility; high reward; high risk; imprint; improved; instrument; light weight; lithography; manufacturing process; nanoplasmonic; nanosensors; non-invasive monitor; noninvasive diagnosis; novel; operation; particle; plasmonics; portability; public health relevance; response; sensor; spectrograph; tool; transmission process

 DESCRIPTION (provided by applicant: Assessment and monitoring of the viral load of HIV+ patients and their adherence to antiretroviral (ART) therapy are critically important for controllig the course of the disease, assessing transmission risks, and monitoring of HIV infection within communities. Viral load measurements are currently conducted in laboratory settings using costly and bulky apparatus including benchtop optical microscopes and sample preparation steps that require advanced lab instruments, which are hard to find and operate in resource limited environments or at the patient's home. In addition to the complexity, cost and bulkiness of existing tools, HIV viral load measurement methods currently rely on blood drawing, which can in general create significant sanitary and safety concerns in resource limited settings or at home. The proposed research plan covers the development and testing of a field-portable and label-free flexible sensing platform that is based on surface plasmon resonant (SPR) properties of specially designed quasi three-dimensional metal nanostructures (MNS) which will be integrated with commercially available soft contact lenses to create a cost effective and non-invasive diagnostic tool that can work even at home for measuring the viral load of HIV+ patients using tear. This new sensing platform will be designed to achieve flexibility by constructing its sensing unit on flexible substrates to enable intimate, direct contact with curved or irregular surfaces such as the human eye without any additional sample retrieval or processing steps and without obscuring natural vision or causing user discomfort. In addition to the flexible plasmonic sensor integrated onto the contact lens surface, we will also create a field portable and cost- effective smart phone based spectral reader platform to specifically analyze and quantify the viral load through multi-spectral imaging and automated analysis of the built-in nano-sensor on the surface of each contact lens. Previous studies have shown that HIV particles exist in tear and ocular fluids of HIV+ patients, and therefore tracking HIV status through tear can present a much safer and simpler approach for monitoring of HIV+ patients and their adherence to ART. In addition to improving care adherence and non- invasively quantifying the viral load of HIV+ patients through tear films, this proposed platform will ultimately provide a simple, cost-effective and light-weight toolset for multiplexed biological and chemical sensing needs, enabling various other future biomedical applications of wearable sensors.

 描述（通过应用提供：评估和监测HIV+患者的病毒负荷及其对抗逆转录病毒（ART）治疗的依从性对于控制疾病的过程至关重要除了现有工具的复杂性，成本和笨重之外，很难在资源有限的环境中找到和运行，目前，艾滋病毒负荷测量方法依赖于血液绘制，这通常可以在资源有限的设置中或在居住的表面上涵盖式式式私人柔韧性（供应范围），这通常会在资源有限的设置中产生大量的卫生和安全性。设计的准三维金属纳米结构（MN）将与市售的软接触透镜集成，以创建一种具有成本效益和非侵入性诊断工具，甚至可以在家中使用撕裂来测量HIV+患者的病毒载荷+患者。这个新的传感平台将旨在通过在柔性基材上构建其感应单元，以实现灵活性 或不规则的表面，例如人眼，没有任何其他样本检索或处理步骤，也不会掩盖自然视觉或引起用户不适。除了集成到隐形眼镜表面上的柔性等离子传感器外，我们还将创建一个磁场 基于便携式且具有成本效益的基于智能手机的光谱读取器平台，可通过对每个隐形眼镜表面上内置的纳米传感器的多光谱成像和自动分析来专门分析和量化病毒载荷。先前的研究表明，艾滋病毒+患者的眼泪和眼部流体存在，因此通过撕裂跟踪艾滋病毒状况可以提出一种更安全，更简单的方法来监测HIV+患者及其对ART的依从性。除了提高护理依从性和非侵入性通过泪膜量化艾滋病毒+患者的病毒载荷外，该提出的平台最终还将提供简单，成本效益和轻巧的工具集，用于多重生物学和多重生物学和 化学感应需求，实现可穿戴传感器的其他各种生物医学应用。

项目成果

Accurate color imaging of pathology slides using holography and absorbance spectrum estimation of histochemical stains.

使用全息术和组织化学染色的吸收光谱估计对病理切片进行准确的彩色成像。

• DOI: 10.1002/jbio.201800335
• 发表时间: 2019
• 期刊: Journal of biophotonics
• 影响因子: 2.8
• 作者: Zhang,Yibo;Liu,Tairan;Huang,Yujia;Teng,Da;Bian,Yinxu;Wu,Yichen;Rivenson,Yair;Feizi,Alborz;Ozcan,Aydogan
• 通讯作者: Ozcan,Aydogan

Smartphone-based turbidity reader.

基于智能手机的浊度读取器。

• DOI: 10.1038/s41598-019-56474-z
• 发表时间: 2019
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: CeylanKoydemir,Hatice;Rajpal,Simran;Gumustekin,Esin;Karinca,Doruk;Liang,Kyle;Göröcs,Zoltan;Tseng,Derek;Ozcan,Aydogan
• 通讯作者: Ozcan,Aydogan

3D imaging of sex-sorted bovine spermatozoon locomotion, head spin and flagellum beating.

• DOI: 10.1038/s41598-018-34040-3
• 发表时间: 2018-10-23
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Daloglu MU;Lin F;Chong B;Chien D;Veli M;Luo W;Ozcan A
• 通讯作者: Ozcan A

Computational Sensing of Staphylococcus aureus on Contact Lenses Using 3D Imaging of Curved Surfaces and Machine Learning.

使用曲面 3D 成像和机器学习对隐形眼镜上的金黄色葡萄球菌进行计算传感。

• DOI: 10.1021/acsnano.7b08375
• 发表时间: 2018
• 期刊: ACS nano
• 影响因子: 17.1
• 作者: Veli,Muhammed;Ozcan,Aydogan
• 通讯作者: Ozcan,Aydogan

Computational Sensing Using Low-Cost and Mobile Plasmonic Readers Designed by Machine Learning.

• DOI: 10.1021/acsnano.7b00105
• 发表时间: 2017-02-28
• 期刊: ACS nano
• 影响因子: 17.1
• 作者: Ballard ZS;Shir D;Bhardwaj A;Bazargan S;Sathianathan S;Ozcan A
• 通讯作者: Ozcan A