Development and regulation of stem cell lineages in skin

皮肤干细胞谱系的发育和调节

• 批准号: 9556552
• 负责人: Isaac Brownell
• 金额: $ 37.31万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9556552
• 关键词: Afferent Neurons; Cell Differentiation process; Cell Lineage; Development; Embryo; Environment; Genetic; Genetic Transcription; Hair follicle structure; Merkel Cells; Mus; Regulation; Sensory; Signal Transduction; Skin; Stem cells; Touch sensation; Work; cell behavior; experimental study; interest; intraepithelial; novel; perineural; stem cell population

Following up on prior work that identified the perineural microenvironment as a regulator of hair follicle stem cells, we have completed a project describing a perivascular niche for hair follicle stem cells. Moreover, we have identified that sensory touch domes in the skin, and the Merkel cells housed there are also regulated by associated Shh signaling from sensory neurons. We have continued experiments demonstrating that touch domes are a novel and distinct cell lineage within the skin maintained by their own stem cell population. We have also investigated the developmental requirements for Merkel cell formation in skin. We have found that establishing the Merkel cell touch dome lineage in embryonic skin requires a cascade of Wnt, Eda, and intraepithelial Shh signaling. Ongoing work investigates the transcriptional requirements for Merkel cell differentiation.

跟进先前的工作，将周围的微环境确定为毛囊干细胞的调节剂，我们已经完成了一个描述毛囊干细胞血管周期壁裂的项目。此外，我们已经确定了皮肤中的感觉接触圆顶，并且所包含的默克尔细胞也受到感觉神经元的相关SHH信号的调节。我们继续进行实验，表明触摸圆顶是皮肤中由其自身的干细胞种群维持的新型细胞谱系。我们还研究了皮肤中默克尔细胞形成的发育要求。我们发现，在胚胎皮肤中建立默克尔细胞触摸圆顶谱系需要一系列Wnt，EDA和上皮内SHH信号传导。正在进行的工作调查了默克尔细胞分化的转录要求。