Transcriptomes and Proteomes of Plasmodium Vivax

间日疟原虫的转录组和蛋白质组

基本信息

批准号：
9241966
负责人：
LIWANG CUI
金额：
$ 16.97万
依托单位：
PENNSYLVANIA STATE UNIVERSITY, THE
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-03-15 至 2018-09-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9241966
关键词：
Achievement Address Affect Area Benign Biological Biology Blood Case Management Central Asia Chloroquine resistance Clinical Clinical Management Clinical Pathology Communities Coupled Culicidae Culture Techniques DNA Microarray Chip Data Development Developmental Biology Disease Epidemiology Erythrocytes Gene Expression Genome Genomics Geography Hepatocyte Hour Human In Vitro Interruption Invaded Kinetics Knowledge Lead Liver Malaria Mass Spectrum Analysis Measures Methods Monkeys Morbidity - disease rate Parasites Pharmaceutical Preparations Plasmodium Plasmodium falciparum Plasmodium vivax Play Population Post-Transcriptional Regulation Preparation Prevalence Primaquine Production Proteins Proteome Proteomics Publishing Recurrent disease Research Resources Reticulocytes Role Sampling Sexual Development Southeastern Asia Sporozoites Surface Antigens Symptoms System Systems Biology Techniques Technology Time Vivax Malaria Work analytical tool asexual base comparative comparative genomics drug discovery gene product humanized mouse improved innovation liver development malaria transmission mortality mouse model neglect novel therapeutics novel vaccines parasite genome public health relevance transcriptome transcriptome sequencing transcriptomics transmission process transmission-blocking vaccine trend vaccine development vaccine discovery

项目摘要

DESCRIPTION (provided by applicant): Plasmodium vivax is the second most prevalent and the most geographically widespread malaria parasite, causing enormous morbidity and mortality in affected populations. Research on P. vivax malaria has lagged significantly behind, largely due to the lack of a continuous in vitro culture system. This parasite selectively invades reticulocytes, making long-term culture difficult. P. vivax gametocytes, which are responsible for transmission, are produced before the onset of the clinical symptoms. Though the first P. vivax genome was published in 2008, systematic comparison of the transcriptomes and proteomes of the asexual erythrocytic cycle is lacking and we know little about the developmental biology of P. vivax gametocytes. This project, built on recent advancement in humanized mouse model to study the liver stages of this parasite and our improved in vitro culture techniques for synchronous blood stages, aims to determine the temporal transcriptomes and proteomes during P. vivax asexual erythrocytic development and gametocytogenesis using RNA sequencing and mass spectrometry technologies, respectively. The comprehensive transcriptomic and proteomic data generated from this study will enable systems biology approaches to address the fundamental biology of asexual and sexual development of this neglected malaria parasite. This information is also deemed critical for the discovery of novel drugs and vaccines for the interruption of P. vivax transmission.

描述（由适用提供）：疟原虫是第二流行和地理广泛的疟原虫，导致受影响人群的发病率和死亡率提高。关于维瓦克斯疟疾的研究已显着落后，这主要是由于缺乏连续的体外培养系统。该寄生虫有选择地侵入网状细胞，使长期文化变得困难。在临床症状发作之前，会产生负责传播的Vivax P. Gametocytes。尽管第一个Vivax基因组于2008年发表，但缺乏无性红细胞周期的转录组和蛋白质的系统比较，我们对维瓦克斯疟原虫配子细胞的发育生物学知之甚少。该项目基于人性化小鼠模型的最新进步，以研究该寄生虫的肝脏阶段以及我们改进的同步血液阶段的体外培养技术，旨在确定使用RNA测序和质谱技术，以相应地分为相对的，确定在体内疟原虫无性促成性发育过程中的临时转录组和蛋白质。这项研究产生的全面转录组和蛋白质组学数据将使系统生物学方法能够解决这种被忽视的疟疾寄生虫的无性和性发育的基本生物学。该信息还被认为对于发现新型药物和疫苗的中断至关重要。