Sex-Specific Gene Expression in Malaria Parasite Plasmodium falciparum

疟疾寄生虫恶性疟原虫的性别特异性基因表达

• 批准号: 8385372
• 负责人: LIWANG CUI
• 金额: $ 22.35万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-22 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8385372
• 关键词: Address; Adverse effects; Antimalarials; Biology; Blood; Cell Separation; Commit; Complex; Coupled; Culicidae; Data; Data Analyses; Development; Erythrocytes; Female; Gender; Gene Expression; Gene Expression Profile; Gene Proteins; Genes; Genomics; Glean; Glucosephosphate Dehydrogenase Deficiency; Goals; Green Fluorescent Proteins; Health; Human; Insecticides; Interruption; Invaded; Knowledge; Lead; Life Cycle Stages; Malaria; Methods; Molecular; Parasites; Pathway interactions; Patients; Pharmaceutical Preparations; Phase; Plasmodium; Plasmodium berghei; Plasmodium falciparum; Population; Post-Transcriptional Regulation; Preparation; Primaquine; Process; Proteins; Proteome; Proteomics; RNA; Reporter; Reporter Genes; Research; Rodent; Role; Sampling; Sex Differentiation; Sexual Development; Shapes; Sorting - Cell Movement; Staging; Systems Biology; Technology; Tubulin; Vaccines; Vision; asexual; comparative; economic impact; improved; male; novel; promoter; scale up; sex; socioeconomics; success; tool; transcriptomics; transmission process; vector; vector mosquito

DESCRIPTION (provided by applicant): With improved financial and technical supports, many malaria endemic nations are once again considering malaria elimination as their national goal of malaria control. To achieve this ambitious goal, one of the greatest challenges is interruption of malaria transmission worldwide. Sexual development of malaria parasite is essential for transmission of the parasite through mosquitoes. Despite its central role in the life cycle of malaria parasites, the mechanism of sexual development is still poorly understood, and this knowledge gap severely hinders the development of novel tools to interrupt malaria transmission. This project, built on our recent success in creating Plasmodium falciparum lines with male or female gametocytes tagged with a reporter gene, aims to determine the molecular differences between the two sexes of gametocytes. The comprehensive transcriptomic and proteomic data generated from this study will enable systems biology approaches to address the fundamental biology of sexual differentiation in malaria parasites. This information is also deemed critical for the development of novel drugs and vaccines for the interruption of malaria transmission. PUBLIC HEALTH RELEVANCE: Sexual development is obligatory for the transmission of malaria parasites through mosquito vectors. Interruption of malaria transmission is considered one of the greatest challenges during the malaria elimination phase. However, the mechanism of sexual differentiation in malaria parasites is still poorly understood. This project is built onour recent success to genetically manipulate the sexual stage parasites and to sort out the two sexes of the gametocyte stages, aiming to determine the differences between the male and female parasite using advanced high-throughput gene expression and protein identification approaches.

描述（由申请人提供）：随着财务和技术支持的改善，许多疟疾的特有国家再次将消除疟疾作为其国家控制的国家目标。为了实现这一雄心勃勃的目标，最大的挑战之一是全球疟疾传播的中断。疟疾的性发育对于通过蚊子传播寄生虫至关重要。尽管它在生活中的核心作用 疟疾寄生虫的循环，性发育的机制仍然很少了解，并且这种知识差距严重阻碍了中断疟疾传播的新工具的发展。这个项目建立在我们最近成功地创建带有记者基因的男性或女配子细胞的恶性疟原虫线上的成功，旨在确定配子细胞的两个性别之间的分子差异。这项研究产生的综合转录组和蛋白质组学数据将使系统生物学方法能够解决疟疾寄生虫性别分化的基本生物学。该信息还被认为对于开发新药物和疫苗的开发至关重要。 公共卫生相关性：性发育对于通过蚊子向量传播疟疾的性发展是必不可少的。疟疾传播的中断被认为是消除疟疾阶段中最大的挑战之一。但是，疟疾寄生虫中性别分化的机制仍然很少了解。该项目是基于最近成功地操纵性阶段寄生虫并整理配子细胞阶段的两个性别的成功建立的，旨在使用先进的高通量基因表达和蛋白质鉴定方法来确定男性和女性寄生虫之间的差异。