MIDBRAIN CIRCUITRY FOR NEURONAL CONTROL OF GAZE

用于注视神经元控制的中脑电路

• 批准号: 9256487
• 负责人: Paul Douglas Gamlin
• 金额: $ 64.38万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9256487
• 关键词: 3-Dimensional; Address; Amblyopia; Anatomy; Animals; Area; Brain; Brain Stem; Cell Nucleus; Cell physiology; Cells; Cerebellum; Characteristics; Ciliary Body; Clinical; Cosmetics; Depth Perception; Disease; Electronics; Etiology; Evolution; Eye; Eye Movements; Functional disorder; Glass; Goals; Grant; Hand; Health; Injection of therapeutic agent; Juice; Knowledge; Label; Location; Macaca; Medial; Midbrain structure; Modeling; Monkeys; Morphology; Motor Neurons; Movement; Needles; Neurons; Oculomotor nucleus; Pathway interactions; Pattern; Phonation; Physiological; Physiology; Play; Pontine structure; Population; Positioning Attribute; Pupil; Rabies virus; Recombinants; Rest; Reticular Formation; Role; Saccades; Shapes; Signal Transduction; Specificity; Strabismus; Structure; System; Techniques; Tectum Mesencephali; Testing; Time; Tracer; Triad Acrylic Resin; Volition; Work; awake; constriction; effective therapy; experimental study; eye hand coordination; frontal eye fields; gaze; improved; lens; neuronal circuitry; novel; public health relevance; recombinant virus; response; sample fixation; skills; superior colliculus Corpora quadrigemina

 DESCRIPTION (provided by applicant): This project will investigate the inputs and physiology of premotor neurons controlling the near triad actions of vergence, lens accommodation and pupillary constriction; critical actions in the etiology of strabismus and amblyopia. It will: 1. provide the first detailed anatomical demonstration of the circuits underlying vergence functions; 2. characterize the function and connections of a novel set of near triad premotor neurons, and 3. test competing current models of eye movement control. The results will confer a better understanding of the mechanisms for obtaining stereoscopic vision. Two opposing models of eye movement control in 3-D space provide the context. One traces its lineage to Hering, who believed that conjugate and vergence eye movement signals were added together at the level of the motoneuron. The other traces its heritage to Helmhotz, who believed that the movement of each eye is independently controlled, and that conjugate and vergence movements represent learned patterns of volitional coordination. We will characterize the physiological responses and determine the inputs to two populations of neurons: perioculomotor vergence cells believed to lie in the supraoculomotor area (SOA) and a newly discovered set of premotor neurons located in the central mesencephalic reticular formation (cMRF). These two populations contact the preganglionic motoneurons in the Edinger-Westphal nucleus (EWpg) that control the lens and pupil, and medial rectus motoneurons active in vergence, indicating a function in near triad control. The study will determine whether the perioculomotor vergence cells and/or premotor cMRF neurons are targeted by the caudal, saccade-related component of the colliculus, the rostral colliculus, which contains cells active during fixation and vergence, or the frontal eye fields (FEF) vergence zone. The 5 inter-related aims carried out in macaque monkeys utilize physiological recording in awake behaving animals, conventional neuronal tracers and transneuronal transport of conventional and recombinant viruses. Aim 1 will compare the precise anatomical location of perioculomotor vergence cells and premotor cMRF neurons. Aim 2 will test the Hering and Helmhotz models by using transneuronal tracing to determine whether these two populations or premotor neurons in the pons are anatomically eye- specific. Aim 3 recordings will test the hypothesis that only the premotor cMRF neurons control the near triad during disjunctive saccades. Aim 4 will examine whether the pattern of tectal projections to these two populations supports such a functional division. Aim 5 will combine physiological and anatomical approaches to ask the same question about FEF inputs. The dramatically augmented understanding of eye movement control circuits and cell function afforded by this project will provide a critical basis for improved concepts of eye movement control and coordination in health and disease.

 描述（由应用程序提供）：该项目将调查控制近距离三合会动作，镜头住宿和瞳孔收缩的近三合会动作的输入和生理；斜视和弱视病因的关键作用。它将：1。提供第一个详细的解剖学演示，内容列出了基础函数的电路； 2。表征一组新型近三合会前神经元的功能和连接，以及3。测试眼动控制的当前模型。结果将使对获得立体视觉的机制有更好的了解。在3D空间中，眼动控制的两个相对模型提供了背景。人们将其血统追溯到Hering，他们认为在Motoneuron的水平上加入了共轭和Gergence眼动信号。另一个追踪其遗产到赫尔姆霍兹（Helmhotz），他认为每只眼睛的运动都是独立控制的，共轭和狂热的运动代表了博学的意志协调模式。我们将表征物理反应，并确定两个神经元群体的输入：周期性肿瘤细胞，被认为位于上关注区区域（SOA）和一组新发现的位于中脑中脑脑脑膜状形成（CMRF）中的新发现的前神经元集。这两个种群接触了控制晶状体和瞳孔的Edinger-Westphal核（EWPG）中的怀孕运动神经元，以及活跃于Gergence的内侧直肠运动神经元，表明在接近三合会控制中具有功能。该研究将确定周期性的传播细胞和/或前CMRF神经元是否受到胶质的尾部，扫视相关成分的靶向，其颈丘丘包含在固定和Vergence期间活跃的细胞，或者是前眼野外（FEF）Vergence vergence Zone。在猕猴中进行的5个相互关联的目的利用了醒着的动物，常规的神经元示踪剂以及常规和重组病毒的跨神经元运输。 AIM 1将比较周期性传播细胞和前CMRF神经元的精确解剖位置。 AIM 2将通过使用跨神经元跟踪来确定PON中的这两个群体或前视神经元在解剖学上是特定于眼睛特异性的，将测试Hering和Helmhotz模型。 AIM 3记录将检验以下假设：只有前CMRF神经元在析出扫视过程中控制接近三合会的假设。 AIM 4将检查这两个人群的剖面项目的模式是否支持这种功能部门。 AIM 5将结合物理和解剖方法，以提出有关FEF输入的相同问题。该项目提供的对眼动控制电路和细胞功能的动态增强理解将为改善健康和疾病的眼动控制和协调的概念提供关键的基础。