600 MHZ NMR RF Console with 19F NMR Probe

带 19F NMR 探头的 600 MHZ NMR RF 控制台

基本信息

批准号：
8640245
负责人：
GAETANO T MONTELIONE
金额：
$ 43.98万
依托单位：
RUTGERS, THE STATE UNIV OF N.J.
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-06 至 2016-05-05
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8640245
关键词：
Address Area Basic Science Biological Biological Sciences Biology Biomolecular Nuclear Magnetic Resonance Biotechnology Cancer Biology Cancer Institute of New Jersey Cellular biology Clinical Research Collaborations Communicable Diseases Communities Comprehensive Cancer Center Computer software Data Analyses Data Collection Dentistry Faculty Frequencies Funding Laboratory Research Malignant Neoplasms Medicine Minor National Institute of General Medical Sciences Neurodegenerative Disorders New Jersey Pharmacologic Substance Principal Investigator Production Protein Structure Initiative Proteins Radio Research Research Institute Research Personnel Running Sampling Solutions Students System Translational Research United States National Institutes of Health Universities Wood material cancer therapy cryogenics drug discovery experience instrumentation medical schools member neurodevelopment programs protein structure ranpirnase research study structural biology structural genomics technology development

项目摘要

7. Project Summary This proposal seeks to fund a state-of-the-art 600 MHz NMR radio frequency (RF) console, with 19F/1H (13C,15N,31P) triple resonance (TR) NMR probe, with capabilities for biological solution NMR. The primary need for this replacement NMR console is to address the limited capacity for NMR data collection at 600 MHz among a broad and growing NMR user group at Rutgers, The State University of New Jersey, and The Robert Wood Johnson Medical School (RWJMS) of the University of Medicine and Dentistry of New Jersey (UMDNJ). The RF console will replace a 16-Yr old Varian INOVA 600 MHz NMR console that has limited capabilities to carry out modern NMR experiments required by our growing NIH-funded NMR user community. The replacement RF console and probe, together with an existing Oxford magnet and cryogenic TR-NMR probe that are already installed, form an integrated biomolecular NMR system. This proposal was prepared on behalf of some 24 research laboratories that will utilize the proposed NMR instrumentation, including major users, minor users, and collaborators. This old console cannot be sustainably maintained due to limited availability of replacement parts and its inability to run modern NMR data collection software. The principal user groups are (i) The Center for Advanced Biotechnology and Medicine (CABM), (ii) The Cancer Institute of New Jersey (CINJ), (iii) The Northeast Structural Genomics Consortium (NESG), and (iv) External Users Group. The CABM is an inter-institutional research institute that is jointly administered by RWJMS and Rutgers University. It serves as hub for scientific interactions throughout the life science research community. CABM includes a group of 13 resident principal investigators who conduct research programs in the broad areas of cancer, infectious disease, and neurodevelopment / neurodegenerative disorders. The CINJ is an NIH- designated P30 Comprehensive Cancer Center, involving some 60 Resident and Associate members. Activities span basic research and pharmaceutical drug discovery, through translational research, clinical research, and cancer treatment. The third user group is the NESG Consortium, funded by the Protein Structure Initiative (PSI-Biology) of the National Institutes of General Medical Sciences. The NESG is one of four Large- scale Centers for Protein Structure Production, supporting a wide range of collaborations in structural biology, cell biology, cancer biology, protein sample production, and technology development. A fourth group of users comprises faculty and students at other universities who take advantage of well-organized data collection and data analysis facilities of the CABM Biomolecular NMR facility. These researchers span groups with limited expertise in protein NMR who send samples and/or students to the facility for data collection and analysis efforts, to experienced established NMR spectroscopists who need access to high field NMR data collection. 1

七、项目概要该提案旨在资助最先进的 600 MHz NMR 射频 (RF) 控制台，配备 19F/1H (13C,15N,31P) 三重共振 (TR) NMR 探针，具有生物溶液 NMR 功能。首要需求此替换 NMR 控制台的目的是解决 600 MHz NMR 数据收集的有限能力罗格斯大学、新泽西州立大学和罗伯特大学拥有广泛且不断增长的 NMR 用户群体新泽西医学和牙科大学 (UMDNJ) 伍德约翰逊医学院 (RWJMS)。 RF 控制台将取代已有 16 年历史的 Varian INOVA 600 MHz NMR 控制台，该控制台的功能有限进行由 NIH 资助的不断壮大的 NMR 用户社区所需的现代 NMR 实验。这替换射频控制台和探头，以及现有的牛津磁体和低温 TR-NMR 探头已安装的设备构成了一个集成的生物分子 NMR 系统。本提案由代表起草约 24 个研究实验室将使用拟议的 NMR 仪器，其中包括主要用户，小用户和合作者。由于可用性有限，这个旧控制台无法持续维护更换零件及其无法运行现代 NMR 数据收集软件。主要用户群体是 (i) 先进生物技术和医学中心 (CABM)，(ii) 新癌症研究所泽西岛 (CINJ)、(iii) 东北结构基因组学联盟 (NESG)、以及 (iv) 外部用户团体。 CABM是一个跨机构研究机构，由RWJMS和罗格斯大学共同管理大学。它是整个生命科学研究界科学互动的中心。 CABM 包括 13 名常驻首席研究员，他们在以下广泛领域开展研究项目癌症、传染病和神经发育/神经退行性疾病。 CINJ 是一个 NIH- 指定的 P30 综合癌症中心，涉及约 60 名住院医师和准会员。活动涵盖基础研究和药物发现，通过转化研究、临床研究和癌症治疗。第三个用户组是 NESG 联盟，由 Protein Structure 资助美国国立普通医学研究院的倡议（PSI-生物学）。 NESG 是四个大型组织之一规模蛋白质结构生产中心，支持结构生物学领域的广泛合作，细胞生物学、癌症生物学、蛋白质样品生产和技术开发。第四组用户由其他大学的教师和学生组成，他们利用组织良好的数据收集和 CABM 生物分子 NMR 设施的数据分析设施。这些研究人员跨越了有限的群体蛋白质 NMR 方面的专业知识，他们将样品和/或学生发送到设施进行数据收集和分析努力，为需要获取高场 NMR 数据收集的经验丰富的 NMR 光谱学家提供帮助。 1

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Minimal Heterochiral de Novo Designed 4Fe-4S Binding Peptide Capable of Robust Electron Transfer.

最小异手性从头设计的 4Fe-4S 结合肽，具有强大的电子转移能力。

DOI：
发表时间：
2018
期刊：
影响因子：
15
作者：
Kim, J Dongun;Pike, Douglas H;Tyryshkin, Alexei M;Swapna, G V T;Raanan, Hagai;Montelione, Gaetano T;Nanda, Vikas;Falkowski, Paul G
通讯作者：
Falkowski, Paul G

Effect of mitochondrial uncouplers niclosamide ethanolamine (NEN) and oxyclozanide on hepatic metastasis of colon cancer.

线粒体解偶联剂氯硝柳胺乙醇胺（NEN）和羟氯扎胺对结肠癌肝转移的影响。

DOI：
发表时间：
2018-02-13
期刊：
影响因子：
9
作者：
Alasadi, Amer;Chen, Michael;Swapna, G V T;Tao, Hanlin;Guo, Jingjing;Collantes, Juan;Fadhil, Noor;Montelione, Gaetano T;Jin, Shengkan
通讯作者：
Jin, Shengkan

Combining Evolutionary Covariance and NMR Data for Protein Structure Determination.

结合进化协方差和 NMR 数据进行蛋白质结构测定。

DOI：
发表时间：
2019
期刊：
影响因子：
0
作者：
Huang, Yuanpeng Janet;Brock, Kelly P;Ishida, Yojiro;Swapna, Gurla V T;Inouye, Masayori;Marks, Debora S;Sander, Chris;Montelione, Gaetano T
通讯作者：
Montelione, Gaetano T

Inhibitor Bound Dengue NS2B-NS3pro Reveals Multiple Dynamic Binding Modes.

抑制剂结合登革热 NS2B-NS3pro 揭示了多种动态结合模式。

DOI：
10.1021/acs.biochem.7b01127
发表时间：
2018-02-15
期刊：
Biochemistry
影响因子：
2.9
作者：
A. Gibbs;R. Steele;Gaohua Liu;B. Tounge;G. Montelione
通讯作者：
G. Montelione

13 C metabolic flux profiling of Pichia pastoris grown in aerobic batch cultures on glucose revealed high relative anabolic use of TCA cycle and limited incorporation of provided precursors of branched-chain amino acids.

在有氧分批培养物中以葡萄糖为基础生长的毕赤酵母的 13 C 代谢通量分析显示，TCA 循环的相对合成代谢使用较高，并且所提供的支链氨基酸前体的掺入有限。

DOI：
发表时间：
2017
期刊：
影响因子：
0
作者：
Zhang, Meng;Yu, Xiao;Xu, Yan;Jouhten, Paula;Swapna, Gurla V T;Glaser, Ralf W;Hunt, John F;Montelione, Gaetano T;Maaheimo, Hannu;Szyperski, Thomas
通讯作者：
Szyperski, Thomas