Regional 800 MHz NMR System Upgrade

区域 800 MHz NMR 系统升级

• 批准号: 10177120
• 负责人: GAETANO T MONTELIONE
• 金额: $ 131.52万
• 依托单位: RENSSELAER POLYTECHNIC INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177120
• 关键词: Address; Biology; Biomolecular Nuclear Magnetic Resonance; Biotechnology; Buffaloes; Capital; Chemical Engineering; Chemicals; Chemistry; Communities; Computer software; Core Facility; Data Analyses; Data Collection; Ensure; Faculty; Failure; Funding; Health Sciences; Institutes; Institution; Maintenance; Manufacturer Name; Measurement; Minor; Modernization; Monitor; NMR Spectroscopy; New Jersey; New York; Pharmacy facility; Policies; Pump; Research; Research Personnel; Resources; Running; Sampling; Science; Scientist; Students; System; Temperature; Time; United States National Institutes of Health; Universities; college; cryostat; experience; experimental study; instrument; radio frequency; success; technology development

7. Project Summary This proposal seeks to fund a state-of-the-art 800 MHz NMR radio frequency (RF) console, [1H/19F,13C,15N,2H]- cryoprobe, cryoprobe cooling unit, and superconducting magnet pump station controller and monitoring unit, as well as a temperature-controlled sample changer accessory, optimally configured for modern solution-state biomolecular NMR. The primary need for this replacement NMR console is to address the critical need for NMR data collection at 800 MHz among a broad and growing user group at Rensselaer Polytechnic Institute (RPI), and at other institutions in the upstate New York and New Jersey region. This NMR spectrometer system will replace a 15-Yr old Bruker Avance II (AVII) 600 MHz NMR console, cryoprobe, and magnet pump controller system that are no longer supported by the manufacturer, and which have limited capabilities to carry out modern NMR experiments required by our growing NIH-funded NMR user community. The replacement RF console and probe, together with an existing well-maintained actively-shielded 18.8 Tesla superconducting magnet with US2 cryostat, will form an integrated biomolecular NMR system. This proposal was prepared on behalf of some 21 research groups, including 14 NIH-funded groups, that will utilize the proposed NMR instrument for measurements. This existing AV II console cannot be sustainably maintained due to limited availability of replacement parts, and its inability to run modern NMR data collection software. The principal user groups include faculty at the Center for Biotechnology and Interdisciplinary Sciences, and in the Departments of Biology, Chemistry and Chemical Biology, and Chemical Engineering at RPI, and external user groups, including faculty of the State University of New York at Albany, the State University of New York at Buffalo, Albany College of Pharmacy and Health Sciences, Sienna College, and Rutgers – The State University of New Jersey. These researchers span groups with limited expertise in NMR who send samples and/or students to the facility for data collection and analysis efforts, to highly-experienced biomolecular NMR spectroscopists who need access to high field NMR data collection. The RPI 800 MHz spectrometer has served for more than 14 years as a critical resource to the capital region of New York, with no other NMR system of this field strength or greater within 120 miles of Troy. The hardware is in urgent need of upgrade, as there is the potential for irreparable hardware failure, and the research groups who use it cannot carry out their NIH-funded research without a state-of-the-art 800 MHz NMR system. Their funded projects are mature, and will utilize new hardware as soon as it is installed. The upgraded instrument will have broad impact, as a significant portion of NMR time will be dedicated to technology development and a broad range of collaborative activities. The outstanding expertise of the scientists responsible for this NMR Core Facility, well-established administration and maintenance policies, commitment to support both major and minor users, and affordable rates for both internal and external users will ensure success of this instrument as a regional resource. 1

七、项目概要 该提案旨在资助最先进的 800 MHz NMR 射频 (RF) 控制台，[1H/19F,13C,15N,2H]- 冷冻探针、冷冻探针冷却装置、超导磁体泵站控制器和监控装置，如 以及温控进样器附件，针对现代溶液状态进行了优化配置 该替代 NMR 控制台的主要需求是满足生物分子 NMR 的关键需求。 伦斯勒理工学院广泛且不断增长的用户群以 800 MHz 进行 NMR 数据收集 (RPI) 以及纽约州北部和新泽西地区的其他机构 该 NMR 波谱仪。 系统将取代已有 15 年历史的 Bruker Avance II (AVII) 600 MHz NMR 控制台、冷冻探头和磁力泵 制造商不再支持且承载能力有限的控制器系统 我们不断壮大的 NIH 资助的 NMR 用户社区所需的现代 NMR 实验 替代 RF。 控制台和探头，以及现有的维护良好的主动屏蔽 18.8 特斯拉超导 磁体与US2低温恒温器，将形成一个集成的生物分子NMR系统。 代表大约 21 个研究小组，其中包括 14 个 NIH 资助的小组，他们将利用拟议的 NMR 由于限制，现有的 AV II 控制台无法持续维护。 更换零件的可用性，以及无法运行现代 NMR 数据收集软件。 用户群体包括生物技术和跨学科科学中心以及 RPI 的生物系、化学系和化学生物学系以及化学工程系和外部用户 团体，包括纽约州立大学奥尔巴尼分校、纽约州立大学奥尔巴尼分校的教师 布法罗、奥尔巴尼药学与健康科学学院、西耶纳学院和罗格斯大学 – 州立 这些研究人员来自新泽西大学，他们在 NMR 方面的专业知识有限，但他们发送样本。 和/或学生到数据收集和分析工作的设施，到经验丰富的生物分子 NMR 需要获取高场 NMR 数据收集的光谱学家 RPI 800 MHz 光谱仪具有。 作为纽约首都地区的重要资源，服务了超过 14 年，没有其他 NMR 特洛伊 120 英里范围内具有这种场强或更大的系统硬件急需升级。 存在不可修复的硬件故障的可能性，并且使用它的研究小组无法执行他们的任务 NIH 资助的研究没有最先进的 800 MHz NMR 系统，他们资助的项目已经成熟，并且。 一旦安装新硬件，升级后的仪器将产生广泛的影响。 NMR 的大量时间将致力于技术开发部分和广泛的协作 负责该 NMR 核心设施的科学家拥有出色的专业知识，已得到完善。 管理和维护政策、支持主要和次要用户的承诺以及负担得起的 内部和外部用户的费率将确保该工具作为区域资源的成功。 1

项目成果

SpecDB: A relational database for archiving biomolecular NMR spectral data.

SpecDB：用于归档生物分子 NMR 光谱数据的关系数据库。

• 发表时间: 2022-09
• 作者: Fraga, Keith J;Huang, Yuanpeng J;Ramelot, Theresa A;Swapna, G V T;Lashawn Anak Kendary, Arwin;Li, Ethan;Korf, Ian;Montelione, Gaetano T
• 通讯作者: Montelione, Gaetano T