Non-Spherical Particles for HPLC

用于 HPLC 的非球形颗粒

基本信息

批准号：
9321117
负责人：
Barry E Boyes
金额：
$ 17.61万
依托单位：
ADVANCED MATERIALS TECHNOLOGY, INC.
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-08-01 至 2018-07-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Extending liquid chromatographic column technology to even higher performance levels has given rise to ultra-high pressure liquid chromatography (UPLC), core-shell particle technology and instrumental developments such as lower volumes for injector and detector hydraulics. In spite of these advances, there is still room for improvement in speed, selectivity and resolution of the liquid chromatographic process. We propose that another level of improvement can be obtained with a change in particle shape by using ellipsoidal particles. These particles offer a reduced pressure drop, a higher mass fraction per unit volume of particles and the possibility to minimize wall effects that are characteristic o packed beds of spherical particles. Furthermore, the possibility of extending this non-spherical particle technology to smaller particle size is important because smaller spherical particles, while offering reduced zone broadening offer larger pressure drops. At some point, the advantage of superficially-porous particle architecture diminishes as particle size is reduced below ≈1.5 µm. If a route to smaller superficially porous non-spherical particles can be devised which minimizes the deleterious pressure drop of spheres, then performance can be increased before the pressure drop causes insurmountable difficulties. New chromatographic particles will be synthesized with a solid ellipsoidal or spherocylinder-like core and then a porous layer will be deposited around the outside for chromatographic retention. We have demonstrated previously in Phase I that there are advantages to this structure with regards to pressure drop and this can be rationalized by bed structures and performance that resemble a monolithic column without the problems of radial inhomogeneity and wall-effect-laden zone broadening that are present in monolithic column technology. We think of the proposed bed structure as that from a "pourable monolith." The current proposal uses synthesis technology and process-scale technology that were discovered and refined during Phase I efforts where it was shown that improved performance can be obtained for larger spherocylinder-like particles that are comparable with smaller spherical particles. In this comparison both the non-spherical and spherical particles used core-shell technology which AMT has pioneered. Phase II will expand on this effort, with the purpose of delivering further improved materials and methods to a broader range of applications in small molecule separations, such as metabolomics, to large molecules, such as proteins, glycoproteins and glycans. The aim here is to not only increase chromatographic resolution, but to make faster separations possible.

描述（由适用提供）：将液相色谱柱技术扩展到更高的性能水平，这导致了超高压力液相色谱（UPLC），核心壳粒子技术和仪器开发，例如注射和检测器水液的较低体积。尽管有这些进步，但液态色谱过程的速度，选择性和分辨率仍然有改善的余地。我们建议通过使用椭圆形颗粒改变粒子形状的另一个改进水平。这些颗粒可提供降低的压降，每单位颗粒体积的质量分数较高，并且有可能最大程度地减少球形颗粒的特征O填充床的壁效应。此外，将这种非球形粒子技术扩展到较小的粒径很重要，因为较小的球形颗粒，同时提供减少区域扩展的可能性提供了较大的压力下降。在某个时候，随着粒径降低以下，超孔颗粒结构的优势减小了。如果可以设计通向较小的超孔非球形颗粒的途径这可以最大程度地减少删除的球体的压力下降，然后在压降会导致无法克服的难度之前提高性能。新的色谱颗粒将与实心椭圆形或类似球形的核心合成，然后多孔层是沉积在外部周围进行色谱保留。我们先前在第一阶段证明了这种结构在压降方面具有优势，并且可以通过类似于整体柱的床结构和性能合理地将其合理化，而没有径向不均匀性和具有壁效应的区域扩展的问题。我们认为拟议的床结构是“可浇注的整体”。当前的提案使用在第一阶段工作中发现和完善的合成技术和过程规模技术，在该技术中，可以证明，可以为较大的类似球形的球形胶样颗粒而获得的性能得到改善，这些颗粒与较小的球形胶样颗粒相当。在这种比较中，非球形和球形粒子都使用了AMT开创性的核心壳技术。第二阶段将扩大这一工作，目的是将进一步改进的材料和方法传递到在小分子分离中（例如代谢组学）中的更广泛的应用，例如蛋白质，糖蛋白和聚糖。这里的目的不仅是增加色谱分辨率，而且是使更快的分离成为可能。