Ellipsoid Particles for High Performance LC Separations

用于高性能 LC 分离的椭球颗粒

基本信息

批准号：
8590657
负责人：
Barry E Boyes
金额：
$ 19.07万
依托单位：
ADVANCED MATERIALS TECHNOLOGY, INC.
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-08-01 至 2014-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8590657
关键词：
Area Biochemical Biological Biological Markers Biological Process Biological Products Biological Sciences Biomedical Research Caliber Chemistry Chromatography Complex Deposition Detection Development Dimensions Disease Evaluation Evolution Family Genetic Glycopeptides Goals High Pressure Liquid Chromatography Hour Inferior Kinetics Knowledge Laboratories Lead Liquid Chromatography Liquid substance Literature Marketing Mass Spectrum Analysis Measurement Methods Molecular Structure Nucleosome Core Particle Particle Size Peptides Performance Permeability Pharmacologic Substance Phase Polysaccharides Post-Translational Protein Processing Process Proteins Proteomics Research Resolution Sampling Science Shapes Silicon Dioxide Solid Structure System Technology Therapeutic Intervention Thick Time Work analytical method drug discovery improved interest novel particle protein expression public health relevance retinal rods self assembly small molecule

项目摘要

DESCRIPTION: Life sciences research and other critical bioanalytical applications would strongly benefit from faster and higher resolution liquid chromatographic (HPLC) separations for both small molecules and larger molecules such as proteins, peptides, glycopeptides, and glycans. Many HPLC separations of biomedical interest, such as proteomic or glycomic profiling, require hours or days for resolution of a single sample. Due to the complexity of biological samples, there are many examples in the current research literature that combine multiple dimensions of HPLC separations, often with online mass spectroscopy (MS). Although very time consuming, this approach has yielded useful information on protein expression and post-translational modifications, the biological processes underlying protein expression and modifications, and the effects thereon of development, disease state, and various environmental and genetic factors. In various complex bioanalytical workflows, high efficiency LC separations are a fundamental part of the analytical systems, and the time to achieve high resolution separations of complex biological samples is a great bottleneck. This proposal describes an approach to improve the separation efficiency of HPLC by extending our proprietary Fused-Core silica platform technology to non-spherical superficially porous particals, including ellipsoids, providing significantly faster and higher resolution separations. Our objectives are to create 2.5-5 ?m diameter ellipsoidal superficially porous Fused-Core silica particles, and to load these materials efficiently into HPLC column formats. The goals of the proposed work could yield very high performance chromatographic products, greater than those currently available, to be applied broadly in analysis of complex biological samples, pharmaceutical and biopharmaceutical applications, in fact in any current application that uses HPLC methods. The separations technology described will directly lead to useful products for which there is a significant technical and market demand.

描述：生命科学研究和其他关键的生物分析应用将使小分子和较大分子（例如蛋白质，肽，糖肽和聚糖）的更快和更高分辨率的液相色谱（HPLC）分离受益。生物医学兴趣的许多HPLC分离，例如蛋白质组学或糖基谱分析，需要小时或数天才能解决单个样品。由于生物样品的复杂性，当前研究文献中有许多例子将HPLC分离的多个维度结合在一起，通常与在线质谱（MS）结合在一起。尽管非常耗时，但这种方法已经产生了有关蛋白质表达和翻译后修饰，蛋白质表达和修饰的生物学过程的有用信息，以及其发育，疾病状态以及各种环境和遗传因素的影响。在各种复杂的生物分析工作流程中，高效率LC分离是分析系统的基本组成部分，而将复杂生物样品进行高分辨率分离的时间是一个很好的瓶颈。该提案描述了一种通过将我们专有的融合核硅平台技术扩展到包括椭圆形的包括椭圆形的多孔部分的非球形式二氧化硅平台技术来提高HPLC的分离效率的方法，从而提供了更快和更高的分辨率分离。我们的目标是创建2.5-5？m直径椭圆形的表面多孔熔融芯颗粒，并将这些材料有效地加载到HPLC柱格式中。所提出的工作的目标可能产生非常高的色谱产品，大于当前可用的生物样品，药物和生物制药应用，实际上在任何使用HPLC方法的应用中，都大致应用于复杂的生物样品，药物和生物制药应用。所描述的分离技术将直接导致有用的技术和市场需求。