Purchase of a Q Exactive HF mass spectrometer system for metabolomics

购买用于代谢组学的 Q Exactive HF 质谱仪系统

基本信息

批准号：
9274622
负责人：
DAVID W. SPEICHER
金额：
$ 60万
依托单位：
WISTAR INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-15 至 2018-04-14
项目状态：
已结题

项目摘要

PROJECT SUMMARY / ABSTRACT This is a proposal to acquire a Thermo Q Exactive HF mass spectrometer and a conventional flow UPLC for a range of metabolomics assays including untargeted (global) metabolite comparisons, flux analyses, and lipidomics profiling (untargeted and targeted). This instrument is needed to support 8 major users and 2 other users at the Wistar Institute to conduct metabolism and lipidome studies involving 22 NIH grants. Eight of these users have multiple NIH grants that will benefit from the requested mass spectrometer system. One of the other users is Dr. Zachary Schug who does not yet have NIH funding because he recently moved from his postdoctoral position in Scotland to the Wistar Institute. Dr. Schug is a critical contributor to this proposal because he has in-depth expertise in all of the analytical approaches that will be used. It is estimated that this user group will initially require nearly all available time on the requested instrument. However, we expect to improve throughput with experience and anticipate that in future years the number of users will grow substantially, thereby ensuring that this instrument will continue to be heavily utilized. Acquisition of this instrument and expansion of our metabolomics capacity is part of a strong institutional commitment driven by the expanding role of metabolism in multiple aspects of cancer biology and other human diseases, coupled with very limited and inadequate access to these technologies from external sources. As a result, metabolism studies at the Wistar Institute have sometimes not been pursued due to logistical barriers and studies that were pursued sometimes suffered from the lack of adequate consultative expertise to ensure optimal experiment design. The requested system will be housed in the Wistar Institute Proteomics and Metabolomics Core Facility under the supervision of the Facility’s Scientific Director, Dr. David Speicher. Day-to-day maintenance and operation of the instrument will be handled by Dr. Hamid Baniasadi, who trained in Dan Raftery’s lab and has more than 8 years of experience with developing and using metabolomics assays. In addition, Dr. Zachary Schug, who is a recently recruited assistant professor from Eyal Gottlieb’s lab, will provide expert consultative advice and will train and educate users through training workshops, a metabolomics journal club, and individual consultation as needed. The Wistar Institute’s CEO and Cancer Center Director confirms the Institute’s very strong commitment to implementing and maintaining state-of-the-art metabolomics capacities. Wistar has already invested more than $1.6M in metabolomics expertise and capacities. Specific to this grant, the Institute is committed to: 1) providing the $66,571 balance of the purchase price above the SIG cap, 2) covering any costs associated with instrument installation, 3) ensuring support is available for expert operators and instrument maintenance throughout its useful lifetime, and 4) ensuring that NIH funded investigators have primary access to the instrument.

项目概要/摘要这是一项购买 Thermo Q Exactive HF 质谱仪和常规流程 UPLC 的提案一系列代谢组学检测，包括非靶向（全局）代谢物比较、通量分析和脂质组学分析（非靶向和靶向）需要该仪器来支持 8 个主要用户和 2 个其他用户。 Wistar 研究所的用户进行代谢和脂质组研究，涉及 22 项 NIH 拨款，其中 8 项。这些用户拥有多项 NIH 拨款，将受益于所请求的质谱仪系统之一。另一位用户是扎卡里·舒格 (Zachary Schug) 博士，他还没有获得 NIH 的资助，因为他最近从自己的实验室搬了出来。苏格兰 Wistar 研究所的博士后职位是该提案的关键贡献者。因为他对将要使用的所有分析方法都有深入的专业知识。用户组最初需要几乎所有可用时间来使用所请求的工具。但是，我们预计通过经验提高吞吐量，并预计未来几年用户数量将会增长。实质上，从而确保该工具将继续得到大量使用。仪器和扩大我们的代谢组学能力是强有力的机构承诺的一部分新陈代谢在癌症生物学和其他人类疾病的多个方面中不断扩大的作用，加上因此，从外部来源获得这些技术的机会非常有限且不充分。威斯塔研究所的研究有时由于后勤障碍而无法进行，而研究有时，缺乏足够的咨询专业知识来确保最佳实验。所请求的系统将安置在 Wistar 研究所蛋白质组学和代谢组学核心中。该设施由该设施的科学总监 David Speicher 博士负责监督。该仪器的操作将由在 Dan Raftery 实验室接受过培训的 Hamid Baniasadi 博士负责此外，Zachary 博士在开发和使用代谢组学检测方面拥有超过 8 年的经验。 Schug 是 Eyal Gottlieb 实验室最近聘用的助理教授，他将提供专家咨询提供建议，并将通过培训研讨会、代谢组学期刊俱乐部和培训用户来培训和教育用户根据需要进行个人咨询。Wistar 研究所首席执行官兼癌症中心主任确认研究所坚定致力于实施和维持最先进的代谢组学能力。 Wistar 已在代谢组学专业知识和能力方面投资超过 160 万美元。具体到本次资助，该研究所承诺：1) 提供高于 SIG 上限的 66,571 美元购买价格余额，2) 承担与仪器安装相关的任何费用，3) 确保为专家操作员提供支持。以及仪器在整个使用寿命期间的维护，以及 4) 确保 NIH 资助的研究人员进行维护。对仪器的主要访问。