Purchase of a Q Exactive HF mass spectrometer system for metabolomics

购买用于代谢组学的 Q Exactive HF 质谱仪系统

基本信息

批准号：
9274622
负责人：
DAVID W. SPEICHER
金额：
$ 60万
依托单位：
WISTAR INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-15 至 2018-04-14
项目状态：
已结题

项目摘要

PROJECT SUMMARY / ABSTRACT This is a proposal to acquire a Thermo Q Exactive HF mass spectrometer and a conventional flow UPLC for a range of metabolomics assays including untargeted (global) metabolite comparisons, flux analyses, and lipidomics profiling (untargeted and targeted). This instrument is needed to support 8 major users and 2 other users at the Wistar Institute to conduct metabolism and lipidome studies involving 22 NIH grants. Eight of these users have multiple NIH grants that will benefit from the requested mass spectrometer system. One of the other users is Dr. Zachary Schug who does not yet have NIH funding because he recently moved from his postdoctoral position in Scotland to the Wistar Institute. Dr. Schug is a critical contributor to this proposal because he has in-depth expertise in all of the analytical approaches that will be used. It is estimated that this user group will initially require nearly all available time on the requested instrument. However, we expect to improve throughput with experience and anticipate that in future years the number of users will grow substantially, thereby ensuring that this instrument will continue to be heavily utilized. Acquisition of this instrument and expansion of our metabolomics capacity is part of a strong institutional commitment driven by the expanding role of metabolism in multiple aspects of cancer biology and other human diseases, coupled with very limited and inadequate access to these technologies from external sources. As a result, metabolism studies at the Wistar Institute have sometimes not been pursued due to logistical barriers and studies that were pursued sometimes suffered from the lack of adequate consultative expertise to ensure optimal experiment design. The requested system will be housed in the Wistar Institute Proteomics and Metabolomics Core Facility under the supervision of the Facility’s Scientific Director, Dr. David Speicher. Day-to-day maintenance and operation of the instrument will be handled by Dr. Hamid Baniasadi, who trained in Dan Raftery’s lab and has more than 8 years of experience with developing and using metabolomics assays. In addition, Dr. Zachary Schug, who is a recently recruited assistant professor from Eyal Gottlieb’s lab, will provide expert consultative advice and will train and educate users through training workshops, a metabolomics journal club, and individual consultation as needed. The Wistar Institute’s CEO and Cancer Center Director confirms the Institute’s very strong commitment to implementing and maintaining state-of-the-art metabolomics capacities. Wistar has already invested more than $1.6M in metabolomics expertise and capacities. Specific to this grant, the Institute is committed to: 1) providing the $66,571 balance of the purchase price above the SIG cap, 2) covering any costs associated with instrument installation, 3) ensuring support is available for expert operators and instrument maintenance throughout its useful lifetime, and 4) ensuring that NIH funded investigators have primary access to the instrument.

项目摘要/摘要这是获取热Q精确HF质谱仪和常规流量UPLC的提议代谢组学测定范围包括未靶向的（全球）代谢物比较，通量分析和脂质组学分析（未靶向和针对性）。需要此工具来支持8个主要用户和其他2个 Wistar Institute的用户进行代谢和脂质群学研究，涉及22个NIH赠款。八个这些用户有多个NIH赠款，可以从请求的质谱仪系统中受益。之一其他用户是Zachary Schug博士，他还没有NIH资金，因为他最近从他的苏格兰的博士后职位。舒格博士是该建议的关键因素因为他在将使用的所有分析方法中都有深入的专业知识。据估计这是用户组最初将需要几乎所有可用仪器上的可用时间。但是，我们希望通过经验改善吞吐量，并预计在未来几年中，用户数量将会增加基本上，确保该工具将继续被大量利用。收购这一点我们的代谢组学能力的工具和扩展是强大的工具承诺的一部分代谢在癌症生物学和其他人类疾病的多个方面的扩大作用，耦合从外部来源访问这些技术的情况非常有限且不足。结果，新陈代谢 Wistar Institute的研究有时由于后勤障碍而没有进行追捕有时会因缺乏足够的咨询专业知识而受到损害以确保最佳实验设计。所请求的系统将安置在Wistar Institute蛋白质组学和代谢组学核心中该设施的科学总监David Speicher博士的监督下的设施。日常维护该乐器的操作将由在Dan Raftery的实验室培训的Hamid Baniasadi博士处理在开发和使用代谢组学测定方面拥有超过8年的经验。此外，Zachary博士舒格（Schug）是最近从Eyeal Gottlieb实验室招募的助理专业人士，将提供专家咨询建议，并将通过培训研讨会，代谢组学期刊俱乐部和根据需要进行个人咨询。 Wistar Institute的首席执行官兼癌症中心主任确认学院致力于实施和维护最先进的代谢组学能力。 Wistar已经投资了超过160万美元的代谢组学专业知识和能力。特定于这笔赠款，该研究所致力于：1）提供高于Sig Cap的购买价格的66,571美元的余额，2）支付与仪器安装相关的任何费用，3）确保专家运营商提供支持和仪器维护整个有用的一生，以及4）确保NIH资助的调查人员拥有对乐器的主要访问。