Van Breemen: Core 1; Botanical Integrity Core (Pauli)

范·布雷曼：核心 1；

• 批准号: 9116102
• 负责人: Guido F Pauli
• 金额: $ 21.73万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9116102
• 关键词: Address; Back; Biological; Biological Assay; Botanical dietary supplements; Botanicals; COSY; Chemicals; Chromatography; Clinical Research; Collaborations; Collection; Communities; DNA; Detection; Ensure; Evaluation; Fingerprint; Fluorescence; Foundations; Funding; Generations; Goals; Industry Standard; Liquid substance; Literature; Methods; Microscopic; Microscopy; Mission; National Center for Complementary and Alternative Medicine; Natural Products; Outcome; Phytochemical; Plants; Policies; Procedures; Production; Protocols documentation; Quality Control; Reference Standards; Reproducibility; Research; Spectrum Analysis; Techniques; Testing; United States National Institutes of Health; Women' s Health; base; complement C2a; countercurrent chromatography; next generation sequencing; novel strategies; solvent extraction

In line with NCCAM/NIH's Policy on Natural Product Integrity and good research practices, Core B will ensure the botanical authenticity and phytochemical reliability of the Center's key study materials: crude herbals, botanical extracts, and purified reference materials (phytochemicals). Core B was established with the 2010 funding cycle of our Center and introduced the Botanical Integrity Dossiers (BPIDs) to ensure traceability and authenticity of the study materials. Core B will continue to fulfill its mission as the Botanical Integrity Core by providing the entire Center with the essential expertise required to ensure the pharmacognostic integrity of the study materials, and will maintain and expand the collection of BPIDs. The overarching goal is to acquire, produce, and certify the pharmacognostic quality of, materials that are essential for the studies in Projects 1-3 and Cores C+D. Core B has three Specific Aims: acquire crude herbal materials, authenticate them, and document their integrity within BPIDs (Aim 1); produce, acquire, and characterize state-of-the-art botanical extracts and generate BPIDs (Aim 2); provide the Center with well-characterized reference materials of purified phytochemicals (Aim 3). For this purpose, Core B will utilize established and modern pharmacognostic methods (organoleptic, microscopy, DNA barcoding), pharmacopoeial, industry-standard and automated extraction procedures (percolation, liquid-liquid partitioning, ASE), and state-of-the art phytochemical methods: preparative chromatography including targeted countercurrent separation (CS), analytical (UHPLC), and spectroscopic techniques (MS, NMR, qNMR, HiFSA). Core B will collaborate closely with the Analytical Core D on the LC-MS and NMR analyses. Collectively, Core B provides an important foundation for the reproducibility and significance of all chemical, biological, and clinical studies in the Center.

符合NCCAM/NIH关于自然产品完整性和良好研究实践的政策，核心B将确保 中心关键研究材料的植物真实性和植物化学可靠性：粗草本， 植物提取物和纯化的参考材料（植物化学物质）。核心B是在2010年建立的 我们中心的资金周期，并引入了植物性完整性档案（BPID），以确保可追溯性和 研究材料的真实性。核心B将继续履行其作为植物完整性核心的使命 为整个中心提供确保的药物认知完整性所需的基本专业知识 研究材料，并将维护和扩展BPID的收集。总体目标是获取， 生产并证明对项目1-3研究至关重要的材料的药物认知质量 和内核C+d。核心B具有三个具体目标：获取原油材料，对其进行身份验证，并 记录其在BPID中的完整性（AIM 1）；生产，获取和表征最先进的植物性 提取并生成bpids（目标2）；为中心提供纯化的纯粹参考材料 植物化学物质（AIM 3）。为此，核心B将利用已建立和现代的药物认知 方法（有机疗法，显微镜，DNA条形码），药水，行业标准和自动化 提取程序（渗透，液体液体分配，ASE）和最先进的植物化学方法： 制剂色谱法，包括靶向反电流分离（CS），分析（UHPLC）和 光谱技术（MS，NMR，QNMR，HIFSA）。核心B将与分析核心D密切合作 在LC-MS和NMR分析上。总体而言，核心B为可重复性提供了重要的基础 中心所有化学，生物学和临床研究的重要性。