Mouse Genome Engineering Core Facility

小鼠基因组工程核心设施

基本信息

批准号：
9338261
负责人：
Channabasavaiah Gurumurthy
金额：
$ 4.2万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9338261
关键词：
Animal Model Biomedical Research Client Complex Consultations Core Facility Development Developmental Process Disease Embryo Embryo Transfer Equipment Faculty Female Funding Future Generations Genetic Genetic Engineering Genetically Engineered Mouse Genome engineering Genotype Goals Human Resources Individual Injection of therapeutic agent Institutes Investigation Investments Knockout Mice Laboratories Learning Medical center Methods Microinjections Micromanipulation Molecular Biology Mouse Strains Mus Nature Nebraska Nobel Prize Operative Surgical Procedures Phase Procedures Recombinants Research Research Infrastructure Research Personnel Resources Services Speed Students System Techniques Technology Time Training Training and Education Transgenes Transgenic Mice Transgenic Organisms United States National Institutes of Health Universities base blastocyst congenic cost effective design embryonic stem cell genome editing improved innovation interest member model design mouse genome mouse model neurosensory new technology pregnant skills success training opportunity transcription activator-like effector nucleases vector

项目摘要

As one of the important scientific cores of the Center for the Molecular Biology of Neurosensory Systems, the goals of Mouse Genome Engineering Core facility (MGECF) are i) to serve all the needs of CoBRE investigators related to the design and creation of transgenic and knockout mouse models, ii) undertake technological research to continuously Improve genetic engineering methods and iii) to provide education and training opportunities to students, researchers and faculty. MGECF was supported by CoBRE during its Phase I at which time the facility was in the initial stages of development. By the end of Phase I the facility was fully functional in creating the mouse models by employing standard mouse embryo manipulation techniques such as microinjection and transferring into pseudo-pregnant females. During the CoBRE phase II period the facility has achieved significant accomplishments: i) started offering critical services such as the design and building of transgenic and targeting vectors that were done previously by the investigators; ii) initiated many other services such as mouse genotyping and speed congenics; iii) acquired additional equipment and personnel and provided training to enhance their overall skills (both scientific and administrative); and iv) initiated research on the most advanced genome engineering methods to validate them for offering as new services. Notably, the MGECF is among the first three such core facilities in the nation that started offering'the genome editing service TALENs (Transcription Activator Like Effector Nucleases). The core almost tripled its user base in 2010 and has maintained it at that level since then. It has has successfully increased its revenue in the recent three years, becoming less dependent of CoBRE support and demonstrating its capability to become sustainable in the future. This core facility is one of the most critical core facilities in the region that serves many investigators across three different institutes. Because of necessity as well as its remarkable success, the MGECF is supported by the UNMC office of the Vice Chancellor for Research. Additionally, the VCR office has a strong commitment to provide support beyond Phase III of CoBRE support.

作为神经感觉系统分子生物学中心的重要科学核心之一，小鼠基因组工程核心设施 (MGECF) 的目标是 i) 满足 CoBRE 的所有需求与转基因和基因敲除小鼠模型的设计和创建相关的研究人员，ii) 承担技术研究不断改进基因工程方法和 iii) 提供教育为学生、研究人员和教师提供培训机会。 MGECF 在其成立期间得到了 CoBRE 的支持第一阶段，该设施处于开发的初始阶段。到第一阶段结束时，该设施通过采用标准小鼠胚胎操作来创建小鼠模型，功能齐全显微注射和移植到假怀孕女性体内等技术。在 CoBRE 阶段第二阶段该设施取得了重大成就：i) 开始提供关键服务，例如研究人员之前完成的转基因和靶向载体的设计和构建；二）启动了许多其他服务，例如小鼠基因分型和速度同类； iii) 获得额外的设备和人员，并提供培训以提高他们的整体技能（科学和技能）行政）; iv) 启动了最先进的基因组工程方法的研究以验证他们提供新服务。值得注意的是，MGECF 是该国首批三个此类核心设施之一。开始提供基因组编辑服务 TALEN（转录激活因子样效应器）的国家核酸酶）。 2010 年，该核心的用户基数几乎增加了两倍，此后一直保持在这一水平。它近三年来成功增加了收入，减少了对 CoBRE 的依赖支持并展示其未来可持续发展的能力。该核心设施是其中之一该地区最重要的核心设施为三个不同研究所的许多研究人员提供服务。由于必要性以及其显着的成功，MGECF 得到了 UNMC 办公室的支持研究副校长。此外，VCR 办公室坚定致力于提供支持超出 CoBRE 支持的第三阶段。