Genetic Studies of Dengue Vectors in Brazil

巴西登革热媒介的遗传学研究

• 批准号: 9221246
• 负责人: Jeffrey R POWELL
• 金额: $ 80.54万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-25 至 2020-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9221246
• 关键词: Address; Adult; Aedes; Affect; Africa; African; Alleles; Area; Back; Brazil; Collection; Competence; Complex; Coupled; Culicidae; Data; Dengue; Disease; Evolution; Female; Freezing; Future; Gene Frequency; Genes; Genetic; Genetic Materials; Genetic Variation; Genetic study; Genome; Genomic Segment; Genomics; Genotype; Geography; Goals; Home environment; Individual; Insecta; Insecticide Resistance; Insecticides; KDR gene; Larva; Location; Malaria; Methods; Pattern; Population; Population Study; Process; Research; Resistance; SNP array; Sampling; Site; Target Populations; Techniques; Time; Transgenic Organisms; Variant; Viral Genome; Virus; Work; follow-up; genetic analysis; genetic makeup; genome wide association study; interest; knock-down; programs; public health relevance; pyrethroid; study population; tool; trait; vector

 DESCRIPTION (provided by applicant): We propose three related projects all focused on control of the major vector, Aedes aegypti, of dengue fever in Brazil. We have developed a powerful new tool for genetic analysis of this species of mosquito, a SNP chip with ~30,000 well-validated SNPs, that will applied in all three projects. The first project is an analysis of a release program using transgenic mosquitoes to suppress population numbers, the so-called RIDL technique. One unanswered question for any such program of this sort is whether the release of such mosquitoes has a lasting impact on the genetic make-up of the natural target population. We have genotyped (using our SNP chip) samples from one site before releases began as well as a sample of the release strain. We will sample the field site 6 months and 12 months after release to examine whether genetic material from the release strain is being transferred to the target population. If so, this has enormous implications for any such future releases of this sort. The second project focuses on studying the genetic underpinning of variation in the ability of female Ae. aegypti to transmit the dengue fever virus, DENV. Our SNP chip coupled with very recent significant advances in genome assembly make this an especially propitious time to undertake such studies. One issue never before addressed in such work is the degree to which populations of mosquito vary geographically in the genes or gene regions responsible for DENV competence. We will also address issues of temporal genetic stability for DENV competence. The third project is on evolution of insecticide resistance in Ae. aegypti in Brazil. Ongoing studies by colleagues at Fiocruz in Rio de Janeiro have revealed very interesting patterns of allele frequencies at the kdr locus responsible for resistance to pyrethroi insecticides. This includes worldwide patterns as well as detailed patterns in Brazil. We propose to continue these studies adding the important adjunct of 30K SNP data from the same samples as studied for kdr alleles. This will provide unprecedented information on evolution of resistance as well as address issues such as the origin of Ae. aegypti populations in Brazil and the timing and origin of insecticide resistance.

 描述（由适用提供）：我们提出了三个相关项目，均集中在巴西的粉丝的主要矢量控制艾德斯·埃及。我们已经开发了一种强大的新工具，用于对这种蚊子的遗传分析，蚊子是一个约有30,000个验证了30,000个SNP的SNP芯片，将在所有三个项目中应用。第一个项目是对 使用转基因蚊子释放程序来抑制人口数量，即所谓的RIDL技术。对于任何此类计划的一个未解决的问题是，这种蚊子的释放是否对自然目标人群的遗传组成产生持久影响。在释放之前，我们已经从一个位点和释放菌株样品中从一个位点进行了基因分型（使用SNP芯片）样品。我们将在释放后6个月零12个月进行对现场地点进行采样，以检查是否将释放菌株的遗传物质转移到目标群体中。如果是这样，这对这种未来的此类版本具有巨大的影响。第二个项目的重点是研究女性AE能力变异的遗传基础。埃及传播粉丝，丹夫。我们的SNP芯片加上基因组组装的最新进展，这使得这是进行此类研究的特别好的时间。这种工作中从未解决的一个问题是，蚊子种群在负责DENV竞争的基因或基因区域上在地理上有所不同。我们还将解决DENV竞争的临时遗传稳定性问题。第三个项目是AE中杀虫剂耐药性的进化。埃及在巴西。在里约热内卢的Fiocruz的同事们正在进行的研究中，在负责对毒性杀虫剂耐药性的KDR基因座的等位基因频率的非常有趣的模式。这包括巴西的全球模式以及详细的模式。我们建议继续这些研究，从与KDR等位基因的Studiod相同的样本中添加了30K SNP数据的重要辅助功能。这将提供有关抵抗进化以及解决AE来源的前所未有的信息。巴西的埃及人口以及杀虫剂耐药性的时间和起源。