Comprehensive characterization of ancestral populations of the vector Aedes aegypti on Indian Ocean islands

印度洋岛屿媒介埃及伊蚊祖先种群的综合特征

• 批准号: 10297945
• 负责人: Jeffrey R POWELL
• 金额: $ 67.79万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-06 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10297945
• 关键词: Address; Admixture; Aedes; Africa South of the Sahara; African; Arboviruses; Biological Assay; Blood; Breeding; Chikungunya virus; Comparative Study; Culicidae; Dangerousness; Data Set; Dengue; Detection; Disease; Documentation; Evolution; Exposure to; Female; Future; Genetic; Genetic Databases; Genetic Variation; Genome; Genomics; Goals; Habitats; Human; Indian Ocean; Indian Ocean Islands; Infection; Insecta; Island; Laboratories; Madagascar; Mammals; Methods; Microfluidics; Morphology; Names; Natural History; Phylogenetic Analysis; Population; Population Genetics; Protocols documentation; RNA; RNA Viruses; Recording of previous events; Research; Resources; Reverse Transcriptase Polymerase Chain Reaction; Sister; Site; Source; Surveys; System; Taxon; Taxonomy; Viral; Virus; Work; Yellow Fever; ZIKA; base; chikungunya; disorder risk; experimental study; forest; human disease; human pathogen; insight; member; multidisciplinary; novel; particle; population genetic structure; reproductive; trait; transcriptome sequencing; vector; vector competence; virome

Project Summary/Abstract We have recently shown that the ancestors of all Aedes aegypti populations in both sub-Saharan Africa as well as around the world presently reside on islands in the Southwest Indian Ocean (SWIO) including Madagascar. These islands gave rise to continental African Ae. aegypti formosus no earlier than about 100,000 years ago. We propose multidisciplinary studies on SWIO populations of Ae. aegypti along with its close relatives, Ae. mascarensis and Ae. pia. This will include population genetics, phylogenetics/phylogeography, vector competence, blood meal analyses, and characterization of the virome of field-caught females. We address question such as: Are the ancestors that gave rise to Ae. aegypti s.s. capable of transmitting the same viruses that cause human diseases? Are these mosquitoes carrying the same viruses in the field and/or do they harbor novel undescribed arboviruses? What are the sources of their blood meals? Where do they breed? Population genetics and phylogenetic preliminary work have already identified candidates for new undescribed species and we suspect more cryptic taxa will be found. We propose to assemble complete genomes for the most informative populations/taxa that will allow comparative studies as well as inference of the ancestral genome of Ae. aegypti s.s., a fundamental resource for analysis of a plethora of genomics studies ongoing in laboratories around the world.

项目摘要/摘要 我们最近表明，撒哈拉以南非洲的所有埃及埃及人口的祖先也 就像目前在世界各地一样，印度洋西南洋（包括马达加斯加）的岛屿。 这些岛屿引起了非洲大陆AE。大约100，000年前的埃及福尔摩斯不早。 我们建议对AE的SWIO人群进行多学科研究。埃及及其近亲Ae。 Mascarensis和Ae。皮亚。这将包括种群遗传学，系统发育学/植物地理学，载体 能力，血液粉分析和野外女性病毒素的表征。我们解决 诸如：祖先会引起AE的问题。埃及S.S.能够传播相同的病毒 那引起人类疾病？这些蚊子是在田间带有相同病毒和/或它们的蚊子 港口小说未描述的arboviruses？他们的血液餐的来源是什么？他们在哪里繁殖？ 人口遗传学和系统发育初步工作已经确定了未描述的新的候选人 物种，我们怀疑会发现更多神秘分类单元。我们建议组装完整的基因组 最有用的人群/分类单元将允许比较研究以及祖先的推理 AE的基因组。 Aegypti S.S.，用于分析大量基因组学研究的基本资源 世界各地的实验室。