Discovery of Natural Products from Botanical Sources

从植物来源发现天然产物

基本信息

批准号：
9280309
负责人：
Patrick C. Still
金额：
$ 14.6万
依托单位：
CALIFORNIA STATE UNIV-DOMINGUEZ HILLS
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-12 至 2020-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9280309
关键词：
AlamarBlue Antineoplastic Agents Biocompatible Materials Biological Assay Botanicals Breast Cancer Cell California Cell Line Cell Survival Chemicals Chemistry Complement Cytotoxic agent Data Disease Evaluation Fruit Future Glean Goals HT29 Cells Human In Vitro Knowledge Lead Los Angeles MDA MB 231 Mass Spectrum Analysis Measurement Mentors Methods Molecular Natural Products Pharmaceutical Preparations Physiologic pulse Phytochemical Plant Extracts Plant Sources Plants Population Primary Health Care Process Research Research Proposals Sampling Source Structure Traditional Medicine Tumor Cell Line Universities Work World Health Organization base cancer cell cytotoxicity design experimental study human disease in vitro Bioassay novel novel therapeutics organic base professor research and development screening technology development

项目摘要

Abstract: “Discovery of Natural Products from Botanical Sources” DESCRIPTION This research is designed to explore anticancer agents from plants. A number of U.S. FDA new drug application (NDA) approvals are from unmodified natural products from plants, synthetic derivatives of plant natural products, and refined plant extracts marketed as a “botanical drug product”. Plants are therefore a rich source of structurally distinct chemical classes with proven utility against disease. In fact, the World Health Organization (WHO) estimates that 65% of the world’s population relies mainly on plant-derived traditional medicines for their primary care. A critical barrier to the progression of phytochemical research, however, is the vast number of plant species unexplored for novel bioactive compounds. This research proposal describes an approach to discover cytotoxic agents from plants. The specific aims of the proposal are as follows: (1) To prioritize samples using cytotoxicity screening, 1H NMR and mass spectrometry to dereplicate known compounds. (2) To employ cytotoxicity-guided isolation towards the discovery of pure lead compounds. (3) To employ comprehensive NMR-based organic structure analysis in the elucidation of isolated lead compounds.

摘要：“从植物来源发现天然产物” 描述本研究旨在探索来自美国 FDA 的多种植物的抗癌剂。新药申请（NDA）批准来自未经修饰的植物天然产物、合成衍生物植物天然产品和精制植物提取物作为“植物药物产品”销售。因此，它是结构独特的化学类别的丰富来源，并且已被证明具有对抗疾病的功效。世界卫生组织 (WHO) 估计，世界上 65% 的人口主要依赖植物来源的食物传统药物用于初级保健是植物化学研究进展的一个关键障碍，然而，这项研究尚未探索大量植物物种的新型生物活性化合物。该提案描述了一种从植物中发现细胞毒性剂的方法该提案的具体目标是。如下： (1) 使用细胞毒性筛选、1H NMR 和质谱对样品进行优先排序，以 (2) 采用细胞毒性引导的分离来发现纯铅 (3) 采用基于核磁共振的综合有机结构分析来阐明分离的铅化合物。