Typhoon FLA 9000 Variable Mode Imaging System

Typhoon FLA 9000 可变模式成像系统

• 批准号: 8246649
• 负责人: A-Lien L Lu-Chang
• 金额: $ 12.8万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246649
• 关键词: Aging; Area; Award; Baltimore; Biochemistry; Biological; Cancer Center; Cell physiology; Computer software; Contract Services; DNA Repair; Data Analyses; Data Collection; Detection; Doctor of Philosophy; Epigenetic Process; Event; Faculty; Fluorescence; Funding; Future; Goals; Grant; Housing; Image; Label; Licensing; Malignant Neoplasms; Maryland; Medical; Molecular; Molecular Biology; Muscle Cells; Nucleic Acids; Principal Investigator; Radio; Radiolabeled; Research; Research Personnel; Research Project Grants; Resources; Sampling; Scientist; Signal Transduction; Students; System; Technology; Training; Training Programs; Transcriptional Regulation; United States National Institutes of Health; Universities; Wages; aged; base; chromatin remodeling; cost; design; human disease; instrument; instrumentation; medical schools; meetings; post-doctoral training; programs; protein protein interaction; radiotracer; repaired; stem cell biology

DESCRIPTION (provided by applicant): NIH-funded principal investigators at the University of Maryland-Baltimore have a pressing need for a variable-mode imaging system to quantitatively detect radio labeled, fluorescently-labeled, and chemifluorescent molecules. The Typhoon FLA 9000 Imaging System is an ideal instrument to meet the needs of our investigators. This instrument is a versatile workhorse for biomolecular imaging applications that supports a wide variety of sample formats. This instrument is requested to replace an aging imaging instrument that is now broken. Repair of this instrument is not feasible, since the repair cost is greater than the instrument value, the instrument is out-dated in terms of capability, and the company cannot guarantee available of parts in the future. The state-of-the-art capability of the Typhoon FLA 9000 instrument is necessary to meet the technology needs of 34 NIH grants (26 R01, 1 P01, 1 R03, 2 R21, 1 RC1, 1 R56, and 2 T32) awarded to 19 users at the UMB School of Medicine. In addition to the instrument, six phosphor screens and two software user licenses are requested to optimize data collection and analysis. The instrument will be housed in the existing Biochemistry and Molecular Biology Instrumentation Core (BMBIC). The BMBIC is designed to support the instrumentation needs of faculty in the northeast quadrant of the University of Maryland-Baltimore campus. The School of Medicine recently invested $400,000 to renovate and expand the BMBIC. The facility is managed by a BMBIC Oversight Committee and this committee directs the efforts of a technical assistant who manages the facility. Dr. Lu-Chang will be the primary faculty resource for technical issues associated with the present instrument. Cost of maintaining the service contract for this instrument will be shared by the School of Medicine, the University of Maryland Greenebaum Cancer Center and the Department of Biochemistry and Molecular Biology. The salary of the technical assistant is guaranteed by the Department of Biochemistry and Molecular Biology. A key long range research goal of all the users is to understand biological events at the molecular level as they pertain to human disease and cancer. The research programs cover multiple areas including protein-protein interaction, protein-nucleic acid interaction, DNA repair, transcriptional regulation, chromatin remodeling, epigenetic, cell signaling, stem-cell biology, and muscle cell physiology. Due to the malfunction of our aged phosphorimager, some of our research projects have suffered serious delays. The Typhoon FLA 9000 will substantially enhance the detection and quantitation of radiolabeled compounds essential for many researchers' experimental strategies, and will also provide investigators with a new fluorescence- and chemifluorescence-based detection platform that will enable them to meet the needs of their NIH-funded programs. Additionally, we believe it is important for students and postdoctoral associates to be trained with the state-of-the-art instrumentation. The new imaging system will enhance the training of students supported by five current NIH-funded graduate/postdoctoral training programs and the MD/PhD medical scientist training program.

描述（由申请人提供）：由 NIH 资助的马里兰大学巴尔的摩分校的主要研究人员迫切需要可变模式成像系统来定量检测放射性标记、荧光标记和化学荧光分子。 Typhoon FLA 9000 成像系统是满足我们研究人员需求的理想仪器。该仪器是生物分子成像应用的多功能主力，支持多种样品格式。该仪器需要更换现已损坏的老化成像仪器。该仪器的维修是不可行的，因为维修费用大于仪器的价值，该仪器的性能已经过时，公司不能保证将来有零件可用。 Typhoon FLA 9000 仪器的最先进功能对于满足 34 项 NIH 拨款（26 R01、1 P01、1 R03、2 R21、1 RC1、1 R56 和 2 T32）的技术需求是必要的UMB 医学院的 19 位用户。除了仪器之外，还需要六个荧光屏和两个软件用户许可证来优化数据收集和分析。该仪器将安装在现有的生物化学和分子生物学仪器核心（BMBIC）中。 BMBIC 旨在满足马里兰大学巴尔的摩校区东北象限教师的仪器需求。医学院最近投资 40 万美元对 BMBIC 进行翻新和扩建。该设施由 BMBIC 监督委员会管理，该委员会指导管理该设施的技术助理的工作。 Lu-Chang 博士将担任与现有仪器相关的技术问题的主要师资资源。维持该仪器的服务合同的费用将由医学院、马里兰大学格林鲍姆癌症中心以及生物化学和分子生物学系分担。技术助理的工资由生物化学与分子生物学系保障。所有用户的一个关键的长期研究目标是在分子水平上了解与人类疾病和癌症有关的生物事件。研究项目涵盖多个领域，包括蛋白质-蛋白质相互作用、蛋白质-核酸相互作用、DNA修复、转录调控、染色质重塑、表观遗传学、细胞信号传导、干细胞生物学和肌肉细胞生理学。由于我们老化的磷光成像仪出现故障，我们的一些研究项目遭受了严重的延误。 Typhoon FLA 9000 将大大增强对许多研究人员的实验策略至关重要的放射性标记化合物的检测和定量，并且还将为研究人员提供一个新的基于荧光和化学荧光的检测平台，使他们能够满足 NIH 的需求资助的计划。此外，我们认为对学生和博士后同事来说，接受最先进仪器的培训非常重要。新的成像系统将加强对由 NIH 目前资助的五个研究生/博士后培训项目和医学博士/博士医学科学家培训项目支持的学生的培训。