The Preterm Infant Microbiome: Biological, Behavioral and Health Outcomes at 2 and 4 years of Age

早产儿微生物组：2 岁和 4 岁时的生物学、行为和健康结果

• 批准号: 9237123
• 负责人: Maureen Edith Groer
• 金额: $ 55.03万
• 依托单位: UNIVERSITY OF SOUTH FLORIDA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-13 至 2020-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9237123
• 关键词: 4 year old; Adult; Affect; Age; Age-Years; Antibiotics; Apoptotic; Bacteria; Biological; Birth; Cesarean section; Child; Childhood; Collection; Colony-forming units; Communities; Consumption; Data; Dependency; Development; Diet; Enrollment; Enteral; Environment; Environmental Risk Factor; Epithelial Cells; Equilibrium; Event; Exposure to; Feces; Food; Gastrointestinal Motility; Genes; Gestational Age; Grant; Growth; Growth Factor; Growth and Development function; Health; Health Status; Home environment; Hospitals; Human Milk; Hypersensitivity; Immune; Immunobiology; Indwelling Catheter; Infant; Infant formula; Infection; Inflammation; Inflammatory; Intestines; Laboratories; Lead; Length of Stay; Leukocyte L1 Antigen Complex; Life; Lymphoid Tissue; Measures; Microbe; Milk; Neonatal Intensive Care Units; Newborn Infant; Oral; Outcome; Oxygen; Physiological; Premature Infant; Procedures; Prospective Studies; Receptor Signaling; Resistance; Risk; Role; Sampling; Science; Secretory Immunoglobulin A; Shapes; Smoking; Source; Steroids; Stimulus; Stress; Structure; Symbiosis; Taxonomy; Testing; Time; Toll-like receptors; Vagina; Very Low Birth Weight Infant; Weaning; Weight Gain; atopy; behavioral health; burden of illness; chemokine; cohort; commensal microbes; cytokine; deep sequencing; donor milk; experience; feeding; gut microbiome; gut microbiota; immune activation; infancy; microbial; microbial community; microbiome; microbiota; milk intake; milk volume; neonate; novel; pathogen; postnatal; prenatal; prospective; public health relevance

 DESCRIPTION (provided by applicant): This project will follow 78 Very Low Birth Weight (VLBW) infants previously enrolled in a R21 grant through the age of 4 years. The data gathered over 6 weeks of the NICU stay includes multiple factors that potentially could alter the gut micro biome, such as prenatal and postnatal events and illnesses, amount of human milk received, weekly means of cytokines, chemokine's, growth factors and secretory Immunoglobulin A in the milk, and weekly levels of fecal calprotectin. Stool samples have been preserved for analysis of the micro biome in the current study. Micro biome species and diversities will be measured in the laboratory of Dr. Jack Gilbert at Argonne National Lab using state of the science deep sequencing and amplification of microbial sRNA genes. The micro biome will again be measured in stool samples from these children at the ages of 2 and 4 years. Relationship between the pre and postnatal factors, human milk volume and immunobiology, fecal calprotectin levels and the very early micro biome will be analyzed. The predictive power of the VLBW infant gut micro biome for determining later childhood micro biomes will be analyzed prospectively. The relationships between micro biomes across time and later growth, development and health will be determined. VLBW infants are at risk for both early and later health effects, and the role of the micro biome in these effects will be measured for the first tim in this prospective study.

 描述（由申请人提供）：该项目将跟踪 78 名之前参加 R21 资助的 4 岁极低出生体重 (VLBW) 婴儿。在 NICU 住院期间收集的 6 周数据包括可能会改变的多种因素。肠道微生物组，例如产前和产后事件和疾病、母乳摄入量、母乳中细胞因子、趋化因子、生长因子和分泌性免疫球蛋白 A 的每周平均值，粪便钙卫蛋白的每周水平已被保存，用于在当前研究中分析微生物群落的种类和多样性，并将在阿贡国家实验室的杰克·吉尔伯特博士的实验室中使用最新的深度测序技术进行测量。微生物 sRNA 基因的扩增将再次测量这些 2 岁和 4 岁儿童的粪便样本中的微生物组，以及产前和产后因素、母乳量和免疫生物学之间的关系。将前瞻性地分析粪便钙卫蛋白水平和非常早期的微生物群落对确定儿童后期微生物群落的预测能力。确定极低出生体重婴儿存在早期和后期健康影响的风险，并且微生物组在这些影响中的作用将在这项前瞻性研究中首次测量。