Unraveling the polymodal behavior of sensory transduction receptors

揭示感觉转导受体的多模式行为

基本信息

批准号：
9126864
负责人：
Paul Garrity
金额：
$ 47.42万
依托单位：
BRANDEIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-02-15 至 2021-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9126864
关键词：
Adult Affect Afferent Neurons Alternative Splicing Animals Anopheles Genus Anopheles gambiae Basic Science Behavior Cells Chemicals Child Clustered Regularly Interspaced Short Palindromic Repeats Culicidae Data Dengue Detection Development Disease Disease Vectors Drosophila genus Drosophila melanogaster Esthesia Family G-Protein-Coupled Receptors Genes Health Homeostasis Humidity Insect Vectors Insecta Light Malaria Mediating Mediator of activation protein Modality Molecular Molecular Probes Neurons Nociceptors Physiology Property Protein Isoforms RNA Splicing Regulation Role Sensitivity and Specificity Sensory Sensory Receptors Specific qualifier value Specificity Stimulus TRPA1 Channel Temperature Temperature Sense Testing To specify Work base behavioral response combinatorial fly gene function genome editing human disease in vivo insect disease insight interest killings member mutant novel promoter public health relevance receptor research study response sensory discrimination vector control water vapor

项目摘要

DESCRIPTION (provided by applicant): Animals detect multiple kinds of stimuli, including chemicals, force, light and temperature. Distinct sensory modalities often rely on related molecular receptors2. In some cases the same receptor participates in multiple sensory modalities. How does such polymodality emerge? How can it be regulated to enable sensory discrimination? We will probe these issues by studying two classes of polymodal receptors we have identified in the fruit fly Drosophila melanogaster whose polymodality is regulated by complementary molecular mechanisms. We propose to investigate these issues in three aims. 1) We will probe the molecular mechanisms by which the activity of a receptor for both moderate warming and aversive chemicals can be regulated in a cell-specific fashion to achieve appropriate sensitivity and specificity, and use these studies to investigate the molecular basis of thermal and chemical detection in both fruit flies and Anopheles mosquitoes. 2) We will investigate how a pair of receptors act together to mediate both thermosensation and hygrosensation, and how these distinct activities are regulated to confer appropriate behavioral responses to each stimulus in both fruit flies and Anopheles mosquitoes. 3) We will test the hypothesis that multiple related receptors can function together in a combinatorial fashion to create distinct classes of sensory receptors that mediate responses to distinct sensory modalities in different sensory neurons in both fruit flies and Anopheles mosquitoes. Together, these studies will provide fundamental insights into the molecular basis of sensory receptor polymodality, how sensory receptors detect thermal and chemical stimuli, and how sensory receptor activity can be regulated to achieve appropriate sensory sensitivity and specificity.

描述（由适用提供）：动物检测到多种刺激，包括化学物质，力，光和温度。不同的感觉方式通常依赖于相关的分子受体2。在某些情况下，相同的受体参与多种感觉方式。这样的多聚糖如何出现？如何受到调节以实现感觉歧视？我们将通过研究我们在果蝇果蝇中鉴定出的两类多峰受体来探讨这些问题，这些果蝇果蝇的多聚峰受到互补分子机制的调节。我们建议以三个目标调查这些问题。 1）我们将探测接收器对中等变暖和厌恶化学物质的活性以细胞特异性的方式调节的分子机制，以达到适当的敏感性和特异性，并利用这些研究来研究两个果实中的热和化学检测的分子基础，这两个果实都将如何在两种果实中进行培养和介导的对旋转的旋转式和旋转的旋转性，并将其呈现为中等的旋转性，这些阶段是对这些阶层的定位，并将其呈现为何种，并将其呈现为何种，并将其呈现为何种，并将其呈现为何种，并将其呈现为何种，并将其呈现为何种，并将其呈现为旋转的旋转性，并将其呈现为旋转的旋转性，并将其呈现型旋转式化。对果蝇和蚊子蚊子中的每种刺激的反应。 3）我们将测试以下假设：多个相关受体可以以组合方式共同发挥作用，以创建不同的感觉受体，这些感觉受体介导了对果蝇和蚊子蚊子中不同感觉神经元中不同感觉神经元中不同感觉方式的反应。总之，这些研究将提供对感觉受体多数型的分子基础的基本见解，感觉受体如何检测热和化学刺激以及如何调节感觉受体活性以达到适当的感觉敏感性和特异性。