MicroRNA Regulation Of Cell Survival In Angiogenesis & Vessel Maturation

血管生成中细胞存活的 MicroRNA 调节

基本信息

批准号：
8843530
负责人：
Sudarshan Anand
金额：
$ 24.49万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8843530
关键词：
Address Affect Angiogenic Factor Apoptosis Apoptotic Attenuated Blood Vessels Cell Death Cell Proliferation Cell Survival Cell physiology Cells Complex Development Disease Endothelial Cells Equilibrium Genome Growth Growth Factor Hemangioma Human In Vitro Inflammation Laboratories Macular degeneration Malignant Neoplasms Mediating Mediator of activation protein Medicine Mentors MicroRNAs Modeling Nature Neonatal Neoplasms in Vascular Tissue Notch Signaling Pathway Pathologic Neovascularization Pathology Pathway interactions Pattern Phase Physiological Processes Process Regulation Research Rest Retinal Diseases Retinal Hemangioma Role Signal Transduction Small RNA Smooth Muscle Myocytes Starvation Stem cells Stimulus Strawberry nevus Stress Tissues Training Tumor Suppressor Genes Untranslated RNA Work Xenograft Model angiogenesis biological adaptation to stress human disease in vivo malformation mouse model notch protein p120 GTPase Activating Protein postnatal programs response retinal angiogenesis

项目摘要

PROJECT SUMMARY/ABSTRACT Aberrant growth of blood vessels termed 'angiogenesis' contributes to the pathology of several human diseases ranging from macular degeneration, retinopathy to inflammation and cancer. Recently small RNAs encoded in the genome termed microRNAs have been implicated in the regulation of diverse physiological processes including angiogenesis. We recently profiled microRNAs during activation of endothelial cells and identified microRNA-132 as a critical regulator of p120RasGAP during pathological neovascularization. In this proposal, I will characterize the role of miR-132/p120RasGAP in cell survival during stress responses in the vasculature using in vitro and in vivo angiogenesis models during the mentored phase. Using these models, in the independent phase, I will analyze the contribution of a different microRNA that functions as a negative regulator of cell survival in vascular cells. These studies will elucidate how distinct microRNA programs govern the balance between the pro and anti-angiogenic states in the vasculature during development and disease.

项目概要/摘要被称为“血管生成”的血管异常生长导致了几种人类的病理学疾病范围从黄斑变性、视网膜病变到炎症和癌症。最近的小RNA 基因组中编码的 microRNA 与多种生理功能的调节有关。过程，包括血管生成。我们最近分析了内皮细胞激活过程中的 microRNA 确定 microRNA-132 是病理性新生血管形成过程中 p120RasGAP 的关键调节因子。在这个提案中，我将描述 miR-132/p120RasGAP 在应激反应期间细胞存活中的作用在指导阶段使用体外和体内血管生成模型来研究脉管系统。使用这些模型，在在独立阶段，我将分析作为负作用的不同 microRNA 的贡献血管细胞中细胞存活的调节剂。这些研究将阐明不同的 microRNA 程序如何进行控制发育和疾病期间脉管系统中促血管生成状态和抗血管生成状态之间的平衡。