FACSAria Fusion Cell Sorter

FACSAria 融合细胞分选仪

基本信息

批准号：
9075287
负责人：
XUEDONG LIU
金额：
$ 59.95万
依托单位：
UNIVERSITY OF COLORADO
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-04-01 至 2017-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We request funds to purchase a high-performance cell sorter coupled with an integrated biological safety cabinet (BSC) to support innovative research by investigators at the University of Colorado-Boulder (UCB). The Becton, Dickinson and Company (BD) FACSAria Fusion is a state-of-the-art high-performance cell sorter capable of multicolor, analyzer-like sensitivity in a custom-fitted biological safety cabinet, designed by The Baker Company, for optimized performance and safety. The proposed instrument will replace a MoFlo Legacy sorter that has been in continuous operation for 16 years in the core facility. Due to the unavailability of parts, and termination of service, the unit can no longer be reliably operated and will soon need to be retired. The past several years CU-Boulder has seen significant growth in the field of biomedical research. Innovations in fluorescence technologies and cell biology during the past decade have dramatically increased the demand of cell sorting capabilities. Our core facility has fallen far behind in meeting the needs of our users due to the age and severe hardware limitations of the MoFlo Legacy sorter. These deficiencies hamper the growth of the core facility to serve the growing requirements of the numerous NIH funded researchers located within JSCBB and throughout the Boulder scientific community. The proposed equipment will allow us to: 1) continue to provide quality services to our existing core user base; 2) address unmet research need to sort and analyze pathogenic and infectious specimens. A number of laboratories in our building and on campus conduct research in this area and there is no cell sorter in the greater Boulder area that has the ability or proper enclosure to sort at biosafety level (BSL) 2+ or 3. This provision is critical for the safe operatin of research goals contained in this proposal; 3) expand our capabilities to serve investigators working on bacteria and yeast. Currently we are the only core facility on campus that serves this group of users; 4) increase our capabilities for multicolor cell sorting and fluorescence modalities such as FRET. Our Legacy sorter can only sort in two wavelengths and there is no cell sorter on campus or nearby with 445 nm and 561 nm laser excitation capabilities, which are ideal for constructs that utilize Cyan and/or Red fluorescent proteins. The replacement of our existing technology with the BD FACSAria Fusion is sorely needed to continue supporting the broad range of NIH funded cutting-edge biomedical research at CU- Boulder. With the new instrument and capabilities, it will empower the users to push new boundaries in their research and spur innovation.

描述（由申请人提供）：我们要求资金购买高性能的CEL，并与综合生物学安全（BSC）相结合，以支持科罗拉多大学 - 波尔顿大学（UCB）的研究人员的创新研究。 BD）FACSARIA FUSION是一个由贝克公司设计的Iological Safety柜，以优化的性能和安全性和服务的终止，该部门不再是可靠地运行，很快就需要退休。遗产分子。基础; 2）未得到的研究需要分类和分析病原和感染性标本。对于本提案中包含的研究目标的安全操作； FRET。令人沮丧的是，在Cu-Boulder的NIH资助范围内，具有新的仪器和能力，它将赋予用户在其研究和刺激创新中推动新的界限。