X-Ray Crylatllography

X射线晶体分析法

• 批准号: 9071332
• 负责人: KAREN E KNUDSEN
• 金额: $ 7.89万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9071332
• 关键词: Affinity; Binding; Biochemistry; Biological; Biological Assay; Biosensor; Calorimetry; Catalysis; Cells; Chromatography; Collaborations; Computer Workstations; Computer software; Consultations; Crystallization; Crystallography; Data; Data Analyses; Devices; Diffusion; Dissociation; Drops; Entropy; Equilibrium; Equipment; Exclusion; Experimental Designs; Fee-for-Service Plans; Fees; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Free Energy; Grant; Heating; Housing; Instruction; Kinetics; Macromolecular Complexes; Malignant Neoplasms; Measurement; Measures; Methods; Minor; Molecular; Molecular Structure; Optics; Phase; Production; Property; Reaction; Reporting; Research Personnel; Resolution; Robot; Roentgen Rays; Sampling; Sedimentation process; Services; Source; Staging; Structure; Surface Plasmon Resonance; System; Technical Expertise; Technology; Temperature; Thermodynamics; Time; Titrations; Training; Translational Research; Tube; United States National Institutes of Health; X ray diffraction analysis; X-Ray Crystallography; base; biophysical techniques; chemical reaction; computerized data processing; computing resources; conformational alteration; data acquisition; enthalpy; instrument; instrumentation; member; millisecond; nano; nanoinstrument; research study; screening; seal; sedimentation velocity; tumor progression

PROJECT SUMMARY (See instructions): The X-ray Crystallography and Molecular Characterization Facility provides access to state-of-the-art x-ray crystallography instrumentation and computational resources, and a wide variety of biophysical approaches lo understanding important molecular interactions. The Facility maintains the equipment and technical expertise to carry out all stages of macromolecular structure determination, and to measure molecular binding parameters such as association and dissociation rates as well as secondary and tertiary structure of biological samples. This facility was used by 6 KCC members.

项目摘要（参见说明）：X 射线晶体学和分子表征设施提供最先进的 X 射线晶体学仪器和计算资源，以及了解重要分子相互作用的各种生物物理方法。该设施拥有进行大分子结构测定各个阶段的设备和技术专业知识，并测量分子结合参数，例如缔合和解离速率以及生物样品的二级和三级结构。该设施由 6 名 KCC 会员使用。