Amygdala-Cerebellum Interactions During Associative Learning

联想学习期间杏仁核-小脑的相互作用

• 批准号: 8881554
• 负责人: John H Freeman
• 金额: $ 32.27万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-02-01 至 2020-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8881554
• 关键词: Alzheimer' s Disease; Amygdaloid structure; Anisomycin; Behavior; Bicycling; Bilateral; Blinking; Bypass; Cell Nucleus; Cerebellum; Conditioned Stimulus; Contralateral; Data; Development; Electric Stimulation; Electrical Stimulation of the Brain; Electrodes; Emotional; Fright; Ipsilateral; Lateral; Learning; Lesion; Memory; Memory impairment; Motor; Muscimol; N-Methyl-D-Aspartate Receptors; Nature; Neurons; Pathway interactions; Performance; Play; Pontine structure; Process; Protein Biosynthesis; Protein Synthesis Inhibition; Rattus; Research; Role; Sensory; Shock; Speed; Stimulus; Stroke; System; Testing; Training; base; classical conditioning; conditioned fear; conditioning; design; long term memory; memory consolidation; motor learning; nervous system disorder; neural circuit; neuromechanism; novel; public health relevance; research study; response

 DESCRIPTION (provided by applicant): Memory deficits are found with many neurological disorders and the breakdown of interactions between memory systems can be particularly debilitating. Different memory systems typically combine and interact to influence everyday behavior. The amygdala and cerebellum have traditionally been viewed as essential players in different types of memory, emotional and motor memory, respectively. These memory systems have been viewed as largely independent. However, recent evidence suggests that the amygdala facilitates cerebellar learning. Our preliminary data indicate that reversible inactivatio of the amygdala in rats severely impairs acquisition and retention of eyeblink conditioning, a type of cerebellar learning. Moreover, amygdala inactivation reversibly impairs the development of learning-related neuronal activity in the cerebellum. This proposal is designed to elucidate the mechanisms underlying interactions between the amygdala and cerebellum. Aim 1 is to determine how the amygdala influences cerebellar function during eyeblink conditioning using reversible inactivation of the amygdala and multi-electrode neuronal recording in the cerebellum. Aim 2 is to determine whether or not the amygdala interacts with the cerebellum through the conditioned stimulus input pathway to the cerebellum using reversible inactivation of the amygdala, axonal tracing, electrical brain stimulation, and multi-electrode neuronal recording. Aim 3 is to determine whether or not amygdala memory consolidation is necessary for facilitating cerebellar learning using protein synthesis inhibition and NMDA receptor blockade in the amygdala and multi-electrode neuronal recording in the pontine nucleus. This project would provide an unprecedented analysis of amygdala-cerebellum interactions.

 描述（由适用提供）：记忆定义存在许多神经系统疾病，并且内存系统之间的相互作用的分解可能会尤其令人衰弱。不同的内存系统通常结合和互动以影响每天的行为。传统上，杏仁核和小脑分别被视为不同类型的记忆，情感和运动记忆中的重要参与者。这些记忆系统已被视为很大程度上是独立的。但是，最近的证据表明，杏仁核设施为小脑学习。我们的初步数据表明，在大鼠中，杏仁核的可逆无活化症严重损害了收购和保留眼睛闪光条件，这是一种小脑学习。此外，杏仁核失活可逆地损害小脑中与学习相关的神经元活性的发展。该建议旨在阐明 杏仁核与小脑之间相互作用的机制。 AIM 1是确定杏仁核如何使用小脑中杏仁核和多电极神经元记录的可逆失活在眉毛调节过程中影响小脑功能。 AIM 2是确定杏仁核是否通过条件刺激输入途径与小脑相互作用，使用杏仁核，轴突跟踪，电气脑刺激和多电极神经元记录的杏仁核，轴突跟踪，电脑脑刺激和多种神经元记录。 AIM 3是确定在杏仁核中开发蛋白质合成抑制和NMDA受体阻滞和蓬托尼核us中多电极神经元记录的NMDA受体阻滞是否需要的。该项目将对杏仁核相互作用进行前所未有的分析。