A new device to improve screening of corneal transplant tissue

改进角膜移植组织筛查的新设备

基本信息

批准号：
8589911
负责人：
Edwin Jay Sarver
金额：
$ 13.24万
依托单位：
SARVER AND ASSOCIATES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-01 至 2016-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8589911
关键词：
Algorithms Anterior Archives Area Cell Count Cell Density Cell Extracts Cells Clinical Trials Computer software Cornea Corneal Endothelium Corneal Topography Corneal edema Custom Data Databases Device Designs Devices Donor Selection Electron Microscopy Elements Endothelial Cells Endothelium Epithelial Evaluation Eye Eye Banks Funding Future Harvest Head Health Housing Human Human Resources Image Image Analysis Keratoplasty Laboratories Laser In Situ Keratomileusis Left Lighting Measurement Measures Metric Microscope Microscopy Operative Surgical Procedures Ophthalmology Optics Patients Pharmaceutical Preparations Plastics Procedures Publishing Research Resolution Retrieval Risk Scanning Shapes Social Work Solutions Specific qualifier value Surgical Flaps Surgical sutures System Techniques Testing Time Tissue Donors Tissue Harvesting Tissues Training Transplantation Transplanted tissue base cellular imaging cost design human tissue improved instrument lens operation prototype public health relevance screening software systems

项目摘要

Marous, James R. An Eye Bank Endothelial Cell Counter Abstract Procurement of donor corneal tissue for transplant use is an important social service undertaken by largely underfunded eye banks. Tissue is not only harvested, but must be screened for a myriad of issues that may make the tissue unsuitable for transplant or pose a health risk to the tissue recipient. Donor tissue may be rejected for morphological issues including prior refractive surgeries (LASIK) or low endothelial cell count. Donor tissue that has undergone a procedure leaving an epithelial flap, such as LASIK, cannot be use for transplant as the flap will separate during suturing. Low endothelial cell count is also a basis for rejection of donor tissue as low endothelial cell count is a precursor to substantial corneal edema. Currently, eye banks screen donor tissue for these issues after the tissue has been harvested either as a whole globe or a corneal-scleral rim. Tissue is returned to the eye bank laboratory and a variety of tests are performed including slit lamp examinations to determine tissue efficacy and specular microscopy to determine endothelial cell count. In a prior funded project, VOL has developed a portable eye scanner device to screen tissue for prior refractive surgeries in the field, prior to tissue harvesting. This device not only generates a definitive metric for the determination of refractive surgery presence, but also reduces the cost associated with tissue harvesting should the tissue be deemed unusable. The current device only scans anterior corneal topography for refractive induced shape changes and tissue that passes this test still requires further examination for endothelial cell count before release for transplant. The current proposal therefore seeks to add the ability to determine endothelial cell count by modifying the existing device to produce a new device that will both scan anterior corneal topography for signs of refractive surgery and perform an endothelial cell count. Such a device will allow screening of both criteria before tissue harvesting thereby saving both significant cost and time.

Marous, James R. An 眼库内皮细胞计数器抽象的采购供移植用的角膜组织是一项重要的社会服务大部分由资金不足的眼库提供。不仅要收获组织，还必须筛选组织许多问题可能使组织不适合移植或对患者构成健康风险组织接受者。供体组织可能因形态问题（包括先前屈光）而被拒绝手术（LASIK）或内皮细胞计数低。经过手术的供体组织留下上皮瓣，例如 LASIK，不能用于移植，因为瓣会分离缝合时。内皮细胞计数低也是排斥供体组织的基础内皮细胞计数是严重角膜水肿的先兆。目前，眼库在采集组织后筛选供体组织是否存在这些问题可以是整个球体，也可以是角膜巩膜边缘。组织返回眼库实验室并进行各种测试，包括裂隙灯检查以确定组织功效和镜面显微镜测定内皮细胞计数。在之前资助的项目中，VOL 开发了一种便携式眼部扫描仪设备，用于筛查组织在现场进行屈光手术之前，在组织采集之前。该装置不仅产生确定屈光手术是否存在的明确指标，同时也降低了成本如果组织被认为无法使用，则与组织采集相关。仅当前设备扫描前角膜地形图以了解屈光引起的形状变化和通过此的组织在释放移植之前，测试仍需要进一步检查内皮细胞计数。因此，当前的提案旨在增加通过以下方式确定内皮细胞计数的能力：修改现有设备以生产可扫描前角膜的新设备地形图检查屈光手术的迹象并进行内皮细胞计数。这样的装置将允许在组织收获之前筛选这两个标准，从而节省大量成本和时间。