Targeted Analysis Resource

有针对性的分析资源

• 批准号: 9062028
• 负责人: John D. Meeker
• 金额: $ 546.64万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9062028
• 关键词: Amino Acids; Analytical Chemistry; Aromatic Polycyclic Hydrocarbons; Benzophenones; Bile Acids; Bioinformatics; Biological; Biological Assay; Biological Markers; Biometry; Chemicals; Child; Child health care; Clinical Research; Communication; Core Facility; Development; Environment; Environmental Epidemiology; Environmental Exposure; Epidemiologic Studies; Equipment; Exposure to; Fatty Acids; Flame Retardants; Goals; Grant; Growth and Development function; Hormones; Human; Human Resources; Immune; International; Isoflavones; Knowledge; Laboratories; Leadership; Lipids; Measurement; Measures; Metabolic; Metals; Methods; Michigan; Micronutrients; Minerals; Molecular Epidemiology; Nonesterified Fatty Acids; Nutrient; Oxidative Stress; Parabens; Pesticides; Phenols; Phytoestrogens; Phytosterols; Polychlorinated Biphenyls; Procedures; Process; Psychosocial Stress; Quality Control; Reproducibility; Research Personnel; Research Support; Resources; Robin bird; Sampling; Science; Technical Expertise; Tobacco smoke; Triclosan; Universities; Validation; Vitamins; Work; bisphenol A; computerized data processing; epidemiology study; epigenomics; experience; inflammatory marker; laboratory facility; metabolomics; method development; novel; nutrition; phthalates; programs; quality assurance; response; sample collection

ABSTRACT (Targeted Analysis Resource) The primary objective of the Michigan Children’s Health Exposure Analysis Resource (M-CHEAR) Laboratory Hub is to contribute to large-scale national efforts to advance our knowledge of the impact of the environment on children’s health by offering high-quality, advanced laboratory support for a wide assortment of targeted and untargeted analyses in biological samples from external researchers conducting epidemiology and clinical studies of children’s health. The overarching goal of the M-CHEAR Targeted Analysis Resource is to provide external investigators with highly sensitive, selective, state-of-the-art targeted analysis of a broad array of biomarkers that can be effectively applied to epidemiology and clinical studies of children’s health. This will be done through leveraging core facilities at University of Michigan and the modern facilities and equipment at its partnering laboratory (NSF International), offering highly sensitive measurement methods that we have already developed in these settings and successfully applied to ongoing high-impact epidemiologic studies of children’s health, implementing and adhering to standard operating procedures and strict quality assurance/quality control processes, continually expanding our analysis capabilities, and effective internal and external communications to respond efficiently to investigator and CHEAR program needs. Specific analyses to be offered include: 1) High-throughput trace level analysis of biomarkers of exposure to a range of environmental exposures, including phthalates, bisphenol A (BPA) and other phenols (e.g., triclosan, benzophenone-3, dichlorophenols), parabens, polycyclic aromatic hydrocarbons (PAH), flame retardant chemicals, persistent and non-persistent pesticides, polychlorinated biphenyls (PCBs), perfluoroalkyl chemicals (PFCs), tobacco smoke, metals/metalloids, and others; 2) Targeted analysis of markers related to nutrition and metabolic factors including mineral nutrients, vitamins, phytoestrogens, lipids, amino acids, fatty acids, bile acids, oxysterols, phytosterols, and more; and 3) Efficient analysis of additional targeted assays of endogenous measures such as hormone levels, immune indicators, and markers of inflammation, oxidative stress, and psychosocial stress. We will work closely with the Developmental Core in the development of methods for targeted analysis of new exogenous or endogenous markers, as well as methods for measurement of these markers in novel biological matrices. The Targeted Analysis Resource will also conduct timely and high-quality data processing to identify potential errors and maximize ease of use, communicate with other CHEAR Laboratory Hubs to capitalize on one another’s capabilities and expertise, and participate in round-robin assessments to determine reproducibility of existing or new methods and to compare accuracy and precision of specific methods across CHEAR Laboratory Hubs. Finally, we will be able to offer our diverse and unique expertise in analysis, interpretation and application of the various biomarkers to epidemiology studies of children’s health to investigators and the CHEAR program.

摘要（目标分析资源） 密歇根儿童健康暴露分析资源 (M-CHEAR) 实验室的主要目标 中心旨在为大规模的国家努力做出贡献，以提高我们对环境影响的了解 通过为各种有针对性的和广泛的儿童健康提供高质量、先进的实验室支持 对外部研究人员进行流行病学和临床的生物样本进行非针对性分析 M-CEAR 目标分析资源的总体目标是提供儿童健康研究。 外部研究人员对一系列广泛的问题进行高度敏感、选择性、最先进的针对性分析 可以有效应用于儿童健康流行病学和临床研究的生物标志物。 利用密歇根大学的核心设施和其现代化的设施和设备来完成 合作实验室（NSF International），提供我们已经拥有的高度灵敏的测量方法 在这些环境中开发并成功应用于正在进行的高影响儿童流行病学研究 健康，执行和遵守标准操作程序和严格的质量保证/质量 控制流程，不断扩展我们的分析能力，以及有效的内部和外部 有效响应研究者和 CEAR 计划需求的沟通。 提供的服务包括：1) 对暴露于一系列环境的生物标志物进行高通量痕量分析 暴露，包括邻苯二甲酸盐、双酚 A (BPA) 和其他酚类（例如三氯生、二苯甲酮 3、 二氯酚）、对羟基苯甲酸酯、多环芳烃（PAH）、阻燃化学品、持久性 和非持久性农药、多氯联苯 (PCB)、全氟烷基化学品 (PFC)、烟草 烟雾、金属/类金属等；2）营养和代谢相关标志物的针对性分析 因素包括矿物质营养素、维生素、植物雌激素、脂质、氨基酸、脂肪酸、胆汁酸、 氧甾醇、植物甾醇等；3) 内源性其他靶向测定的有效分析 激素水平、免疫指标以及炎症、氧化应激和炎症标志物等指标 我们将与发展核心密切合作，制定应对心理压力的方法。 新的外源或内源标记物的靶向分析，以及这些标记物的测量方法 靶向分析资源也将及时、高质量地进行。 数据处理以识别潜在错误并最大限度地提高易用性，与其他 CHEAR 进行通信 实验室中心利用彼此的能力和专业知识，并参与循环赛 评估以确定现有或新方法的再现性并比较准确性和精密度 最后，我们将能够提供多样化且独特的方法。 分析、解释和应用各种生物标志物进行流行病学研究的专业知识 儿童健康问题向调查人员和 CEAR 计划提出。