Innovations in functionality and manufacturability of IsoRaft Arrays

IsoRaft 阵列的功能和可制造性方面的创新

• 批准号: 8590811
• 负责人: Nicholas Carl Dobes
• 金额: $ 15万
• 依托单位: CELL MICROSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8590811
• 关键词: Address; Adoption; Animal Model; Area; Biocompatible; Biological; Biological Sciences; Biomedical Research; Biotechnology; Blood Cells; Cell Culture Techniques; Cell Line; Cell Separation; Cells; Cellular biology; Cloning; Collection; Communities; Core Facility; Cultured Cells; Cycloparaffins; Development; Devices; Drug Industry; Elements; Encapsulated; Ensure; Feedback; Fluorescence; Generations; Genetic Engineering; Glass; Goals; Grant; Health; Housing; Image; Institutes; Intellectual Property; Licensing; Marketing; Mechanics; Methods; Mission; Needles; North Carolina; Optics; Patients; Phase; Plastics; Polymers; Population; Procedures; Production; Property; Research; Research Institute; Sample Size; Signal Transduction; Small Business Innovation Research Grant; Solid; Sorting - Cell Movement; Stem cells; Sterility; Surface; Suspension substance; Suspensions; System; Technology; Testing; Time; Translations; United States National Institutes of Health; Universities; Valinomycin; Water; absorption; base; biomaterial compatibility; cell type; cellular imaging; copolymer; cost; cost effective; flexibility; forest; genetically modified cells; improved; innovation; interest; microsystems; novel; physical separation; polydimethylsiloxane; programs; prototype; public health relevance; research study; screening; success

DESCRIPTION (provided by applicant): Cell Microsystems is a North Carolina-based start-up biotechnology company whose mission is to commercialize a novel, yet affordable, platform for the efficient isolation of viable, single cells or colonies from a mixed population while the cell/colony remains adherent or encapsulated to a solid surface. The company's "IsoRaft" technology is based on a unique cell array recently developed at the University of North Carolina (UNC) at Chapel Hill, and represents an ideal opportunity for the translation of an academic technology to the marketplace through the SBIR program. Cell Microsystems has obtained license from UNC to commercialize the technology for a broad market in academic labs, as well as in the biotechnology and pharmaceutical industries. The products consist of disposable microarray (the IsoRaftTM Array) for culturing cells and a simple device for isolating the cell/colony of interest. Prototypes of arrays and devices have been completed and are being tested in a number of academic labs as an Early Adoption Program (EPA) at nearby research institutes. In this Phase I SBIR proposal, improvements will be made in the IsoRaft" array that address feedback from our market needs analysis that will also dramatically improve the manufacturability of the array. Particularly we will institute new materials and a method of manufacture that will enable commercial fabrication of a flat, transparent, biocompatible array of micro-molded elements. The use IsoRaft technology for cell culture, imaging and isolation applications will be conducted by our academic collaborator at UNC. Our goal is to expand the use of this technology for cell research to the large community in the life science market. The studies in Phase I will deliver a 2nd generation consumable array that can be mass manufactured to provide a flexible, powerful, yet cost-effective means to perform unique cloning and sorting experiments at significant reductions in cost and time.

描述（由申请人提供）：Cell Microsystems 是一家总部位于北卡罗来纳州的初创生物技术公司，其使命是将一种新颖且价格实惠的平台商业化，用于从混合群体中有效分离活的单细胞或集落，同时细胞/菌落保持粘附或封装于固体表面。该公司的“IsoRaft”技术基于北卡罗来纳大学教堂山分校 (UNC) 最近开发的独特单元阵列，代表了通过 SBIR 计划将学术技术转化为市场的理想机会。 Cell Microsystems 已获得北卡罗来纳大学的许可，可以将该技术商业化，应用于学术实验室以及生物技术和制药行业的广阔市场。该产品包括用于培养细胞的一次性微阵列（IsoRaftTM 阵列）和用于分离感兴趣的细胞/集落的简单装置。阵列和设备的原型已经完成，并作为附近研究机构的早期采用计划 (EPA) 在许多学术实验室进行测试。在第一阶段 SBIR 提案中，将对 IsoRaft" 阵列进行改进，以满足我们市场需求分析的反馈，这也将极大地提高阵列的可制造性。特别是，我们将开发新材料和制造方法，使商业化成为可能我们在北卡罗来纳大学的学术合作者将使用 IsoRaft 技术制造扁平、透明、生物相容的微模制元件阵列，我们的目标是扩大该技术在细胞研究中的应用。第一阶段的研究将向生命科学市场的广大社区提供第二代耗材阵列，该阵列可以批量生产，以提供一种灵活、强大且经济高效的方法来执行独特的克隆和分选实验，并显着降低成本。成本和时间。