CellRaft Arrays for Single-cell PCR

用于单细胞 PCR 的 CellRaft 阵列

• 批准号: 8824733
• 负责人: Nicholas Carl Dobes
• 金额: $ 18.98万
• 依托单位: CELL MICROSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-26 至 2016-03-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8824733
• 关键词: Bacteria; Basic Science; Biological; Biological Assay; Biological Sciences; Biopsy; Biotechnology; Cell Separation; Cells; Characteristics; Collaborations; Collection; Communities; Complex; Core Facility; Crime; Cultured Cells; Development; Devices; Disease; Drug Industry; Environment; Europe; Forensic Medicine; Funding; Genomics; Goals; Grant; Health; Heterogeneity; Individual; Intellectual Property; Knowledge acquisition; Laboratories; Laboratory Research; Letters; Licensing; Link; Marketing; Measurement; Methods; Microscope; Mission; Molecular Analysis; North Carolina; Phase; Polymerase Chain Reaction; Population; Preparation; Protocols documentation; Publications; Research Institute; Sample Size; Sampling; Small Business Innovation Research Grant; Solid; Sorting - Cell Movement; Sterility; Surface; System; Techniques; Technology; Testing; Tissues; Transcript; Translations; Tube; United States National Institutes of Health; Universities; Virus; base; biological research; cell behavior; cost; cost effective; disease diagnosis; forest; genetic analysis; improved; innovation; interest; microsystems; novel; product development; programs; public health relevance; research and development; research study; screening; single cell analysis; tool

DESCRIPTION (provided by applicant): Cell Microsystems is a start-up biotechnology company whose mission is to commercialize a cost effective platform for isolating and manipulating single, viable cells. The company's CellRaft(tm) technology is based on a unique cell array recently developed at the University of North Carolina (UNC) at Chapel Hill, and represents an ideal opportunity for the translation of an academic technology to the marketplace through the NIH sponsored SBIR partnership. In recognition of the growing market of single-cell genetic analysis, we will explore the feasibility of using the CellRaft technology as a sample handling tool for the analysis of single cells by polymerase chain reaction (PCR). The CellRaft(tm) technology is unique in enabling individual cells to be identified by a variety of selection critera followed by isolation with extremely high viability, purity, and yield. In this Phase I SBIR proposal, we will optimize the CellRaft(tm) Array for single-cell isolation towards the applicationof PCR, and optimize the collection and transfer method to deliver single cells from the array to a vessel for conducting PCR. Our academic collaborator at UNC will conduct the cell-based experiments to implement the CellRaft(tm) technology for single-cell PCR analysis through the use of a commercial single-cell PCR kit. The product will be specifically developed to be compatible with existing commercial single-cell PCR kits in collaboration with a company who is a world leader in sample handling and assay products. This collaboration will utilize the company's commercial single cell PCR kit. The studies in Phase I will deliver a market-ready CellRaft Array product optimized and specific for single-cell PCR applications. Our goal is to bridge the CellRaft Array technology with single-cell PCR analysis and promote the use of this technology to the large and growing community in the life science market interested in the molecular analysis of single cells.

描述（由申请人提供）：Cell Microsystems 是一家初创生物技术公司，其使命是将用于分离和操作单个活细胞的具有成本效益的平台商业化。该公司的 CellRaft(tm) 技术基于北卡罗来纳大学教堂山分校 (UNC) 最近开发的独特细胞阵列，代表着通过 NIH 赞助的 SBIR 合作伙伴关系将学术技术转化为市场的理想机会。鉴于单细胞遗传分析市场的不断增长，我们将探索使用 CellRaft 技术作为样本处理工具通过聚合酶链式反应 (PCR) 分析单细胞的可行性。 CellRaft(tm) 该技术的独特之处在于，能够通过各种选择标准来识别单个细胞，然后以极高的活力、纯度和产量进行分离。在此第一阶段 SBIR 提案中，我们将优化用于 PCR 应用的单细胞分离的 CellRaft(tm) 阵列，并优化收集和转移方法以将单细胞从阵列输送到容器中进行 PCR。我们在北卡罗来纳大学的学术合作者将进行基于细胞的实验，通过使用商业单细胞 PCR 试剂盒实施 CellRaft(tm) 技术进行单细胞 PCR 分析。该产品将与一家在样品处理和检测产品领域处于世界领先地位的公司合作专门开发，以便与现有的商业单细胞 PCR 试剂盒兼容。此次合作将利用该公司的商业单细胞 PCR 试剂盒。第一阶段的研究将提供一款面向市场的 CellRaft 阵列产品，该产品经过优化且专门针对单细胞 PCR 应用。我们的目标是将 CellRaft 阵列技术与单细胞 PCR 分析结合起来，并将该技术推广到生命科学市场中对单细胞分子分析感兴趣的庞大且不断增长的群体中。