Transcending dynamic and kinetic limits for neuronal calcium sensing

超越神经元钙传感的动态和动力学限制

基本信息

批准号：
8912632
负责人：
Samuel Sheng-Hung Wang
金额：
$ 24.3万
依托单位：
PRINCETON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-02-01 至 2017-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8912632
关键词：
Action Potentials Affinity Animals Axon BRAIN initiative Binding Biological Brain Brain Diseases Bypass Calcium Calcium Signaling Calculi Calmodulin Cells Chimeric Proteins Computer Simulation Consult Data Dendrites Development Diagnosis Disease Electrophysiology (science)Fluorescence Future Goals Green Fluorescent Proteins Health Image Imaging Techniques Imaging technology Individual Interneurons Kinetics Knowledge Laboratories Lead Learning Location Methods Microfluidic Microchips Mission Modeling Monitor Mutation National Institute of Neurological Disorders and Stroke Neurons Neurosciences Optics Pathway interactions Prevention Process Property Public Health Reporting Research Research Personnel Resolution Rewards Signal Transduction Speed Structural Biologist Surface System Technical Expertise Techniques Time Tissues Training Transcend Variant Work awake base brain circuitry brain tissue burden of illness calcium indicator cell type combinatorial design granule cell high throughput screening improved innovation interest motor control neocortical neural circuit new technology novel strategies optical imaging public health relevance relating to nervous system research study response screening small molecule success tool

项目摘要

DESCRIPTION (provided by applicant): Many pathways that carry signals from one neuron to another involve calcium as a messenger molecule. By examining changes in calcium concentration within individual neurons, researchers can learn how these neurons interact within circuits. A long-term goal of this laboratory is to produce indicators that can track the full rang of calcium changes in large numbers of neurons in behaving animals across days to weeks. In particular, the proposed experiments will produce new technology that can follow activity in neuronal cell types that are involved in important brain functions, from fine motor control to reward learning. The overall objective of this application is to design probes that can follow rapi neural activity in awake, behaving animals and to demonstrate their usefulness in studying cell types whose activity cannot be recorded using currently available techniques. This contribution is significant because it will allow researchers to investigate the function of important neural circuits under realistic conditions. This approach is innovative because this laboratory will develop tools that allow the study of cells that previously could not be examined in awake animals. The work proposed in this application will therefore advance our ability to understand how the brain functions in health and disease. In the long run, this information could lead to new approaches to diagnosis, treatment, and prevention of a variety of brain disorders.

描述（由申请人提供）：将信号从一个神经元传递到另一个神经元的许多途径都涉及钙作为信使分子，通过检查单个神经元内钙浓度的变化，研究人员可以了解这些神经元如何在回路中相互作用。该实验室的目标是生产能够追踪行为动物中大量神经元在数天到数周内钙变化的指标，特别是，拟议的实验将产生可以跟踪参与的神经元细胞类型活动的新技术。重要的大脑功能，从精细该应用的总体目标是设计能够跟踪清醒、行为动物的rapi神经活动的探针，并证明它们在研究使用当前可用技术无法记录的细胞类型中的有用性。因为它将允许研究人员在现实条件下研究重要神经回路的功能，因为该实验室将开发出能够研究以前无法在清醒动物中进行检查的细胞的工具。因此，提高我们了解大脑在健康和健康状态下如何运作的能力从长远来看，这些信息可能会带来诊断、治疗和预防各种脑部疾病的新方法。