Exposure core

曝光核心

• 批准号: 8926437
• 负责人: Carl W White
• 金额: $ 13.01万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8926437
• 关键词: Academic Medical Centers; Acids; Acute Lung Injury; Aerosols; Air; Alteplase; Anesthesia procedures; Anesthetics; Animal Experiments; Animal Housing; Animals; Anticoagulants; Antioxidants; Appearance; Area; Autopsy; Biochemical; Blood capillaries; Body Fluids; Breathing; Caliber; Certification; Chemical Models; Chemicals; Chlorine; Collaborations; Color; Colorado; Computers; Conscious; Cross-Over Studies; DNA Damage; Data; Distress; Edema; Ensure; Environmental air flow; Evaluation; Event; Exposure to; Free Radicals; Gases; Head; Health; Hour; Human Resources; Inflammation; Injury; Intervention Studies; Laboratories; Laboratory Procedures; Liquid substance; Lung; Maintenance; Mechlorethamine; Medical; Methods; Modeling; Monitor; Mustard; Mustard Gas; New Agents; Nose; Operative Surgical Procedures; Perfusion; Peripheral; Pharmaceutical Preparations; Plasminogen Activator; Poison; Preparation; Procedures; Process; Protocols documentation; Publications; Pulmonary function tests; Pulse Oximetry; Qualifying; Quality Control; Rattus; Records; Regulation; Reporting; Research Personnel; Resources; Rodent; Safety; Sampling; Site; Skin; Stethoscopes; Structure of parenchyma of lung; Supervision; System; Techniques; Testing; Therapeutic Intervention; Thrombolytic Therapy; Time; Tissue Procurements; Toxic effect; Travel; Universities; Vertebrates; Vesicants; Weight; Whole Body Plethysmography; Work of Breathing; Writing; animal care; base; capillary; chlorine gas; computerized data processing; data modeling; follower of religion Jewish; health data; health record; in vivo; interest; lung injury; member; novel therapeutics; programs; protective effect; research study; silibinin; sound

The Exposure Core consists of an AinA/ay (Lung) Exposure Core based in Denver at National Jewish Health and a Skin Exposure Core based at University of Colorado Denver on the medical campus. The Ainway (Lung) Exposure Core consists of an offsite exposure facility at USAMRICD (Aberdeen, MD) subcontracted for exposure of rats to SM (described herein) supervised by our collaborator Dana Anderson, and Denver (National Jewish Health)-based exposure facilities for ain/vay exposures to half-mustard (2-chloroethyl ethylsulfide, CEES; nose-only system) and chlorine (whole body). The former provides an ethanolic aerosol (0.6 micron mass median diameter) by nose-only delivery to anesthetized rats in an established model. The latter provides chlorine gas in dry dilution air by whole body exposure to conscious rats in a more recently constructed system that is functioning and providing results similar to those reported by others with similar levels of exposure. Verification of exposure levels in the chlorine system is based on the 'sulfamic acid trap' method previously described Rando and colleagues. Processing of lung tissue and body fluids from rats exposed to these three toxic inhaled gases is illustrated in schematic diagram. For studies requiring airway instillation of drugs (eg tPA) with SM exposure, a small group of investigators (1) and technicians (2) from National Jewish will travel to USAMRICD 2-4 times annually to assist in these more labor-intensive studies. Pulmonary function testing (flexiVent) is available nearby to CEES and chlorine exposure systems in Denver, and also at the SM facility in Aberdeen (Buxco whole body plethysmography). The latter is described herein and the former is in Project 1. The Ainway Exposure Core is heavily used mainly by Projects 1 and 2. The Skin Exposure Core during the first cycle established an in vivo exposure system for CEES that can cause reproducible microvesication, DNA damage, inflammation and edema, and more recentiy has developed a nitrogen mustard exposure system that results in all of these events but with much more marked potency. The Core includes interactive studies will all 3 Projects.

暴露核心由位于丹佛国家犹太健康中心的 AinA/ay（肺）暴露核心和位于科罗拉多大学丹佛分校医学院的皮肤暴露核心组成。 Ainway（肺）暴露核心由位于 USAMRICD（马里兰州阿伯丁）的异地暴露设施组成，该设施分包给大鼠暴露于 SM（本文所述），由我们的合作者 Dana Anderson 监督，以及位于丹佛（国家犹太健康中心）的暴露设施，用于ain/vay 暴露于半芥末（2-氯乙基 乙硫醚，CEES；仅鼻子系统）和氯（全身）。前者通过仅鼻递送至已建立模型中的麻醉大鼠来提供乙醇气雾剂（0.6微米质量中值直径）。后者通过在最近构建的系统中将全身暴露于有意识的老鼠，在干燥稀释空气中提供氯气，该系统正在运行并提供与其他类似情况报告的结果相似的结果。 暴露程度。氯系统中暴露水平的验证基于 Rando 及其同事之前描述的“氨基磺酸陷阱”方法。示意图显示了暴露于这三种有毒吸入气体的大鼠的肺组织和体液的处理。对于需要气道滴注药物（例如 tPA）并暴露 SM 的研究，一小组研究人员 (1) 和技术人员 (2) 来自 National Jesus 每年将前往 USAMRICD 2-4 次，以协助这些更加劳动密集型的研究。 丹佛的 CEES 和氯暴露系统附近以及阿伯丁的 SM 设施（Buxco 全身体积描记法）均可进行肺功能测试 (flexiVent)。后者在本文中进行了描述，前者在项目 1 中进行了描述。Ainway Exposure Core 主要由项目 1 和 2 大量使用。皮肤暴露核心在第一个周期中建立了 CEES 的体内暴露系统，可导致可重复的微泡、DNA损伤、炎症和水肿，最近开发了一种氮芥暴露系统，该系统会导致所有这些事件，但效力更显着。核心课程包括所有 3 个项目的互动研究。