Neural Substrates of Electroacupuncture in Cardiovascular Control

电针控制心血管的神经基质

• 批准号: 8874256
• 负责人: John C Longhurst
• 金额: $ 38.05万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8874256
• 关键词: Abbreviations; Acupuncture Therapy; Acute; Adverse effects; American; Animals; Area; Automobile Driving; Biologic Characteristic; Blood Pressure; Brain; Cardiovascular system; Cell Nucleus; Cholecystokinin A Receptor; Cholecystokinin Receptor; Clinical; Conscious; Data; Disease; Electroacupuncture; Endorphins; Enkephalins; Figs - dietary; Genes; Glutamates; Goals; Grant; Hour; Hypertension; Hypotension; Hypothalamic structure; Incidence; Investigation; Knowledge; Medical; Messenger RNA; Methods; Midbrain structure; Modality; Modeling; Molecular; Morbidity - disease rate; Myocardial Ischemia; National Center for Complementary and Alternative Medicine; National Heart, Lung, and Blood Institute; Neurons; Neurotransmitters; Opioid; Oranges; Pathway interactions; Patients; Physicians; Physiological; Play; Population; Pro-Opiomelanocortin; Process; Production; Rattus; Reflex action; Regulation; Research; Role; Spinal Cord; Standardization; Structure of nucleus infundibularis hypothalami; Tetragastrin; Text; Thoracic spinal cord structure; Treatment Protocols; Visceral; Whole Organism; aged; blood pressure reduction; blood pressure regulation; hemodynamics; hypertension treatment; improved; individualized medicine; insight; mRNA Expression; midbrain central gray substance; mortality; neuromechanism; neurophysiology; neuroregulation; paraventricular nucleus; preproenkephalin; raphe nuclei; relating to nervous system; response

DESCRIPTION (provided by applicant): We have investigated the mechanisms by which acupuncture, particularly electroacupuncture (EA) regulates blood pressure (BP). EA has the capability to lower elevated BP and to alleviate demand-induced myocardial ischemia. Its actions are slow in onset but its duration of action is prolonged. Approximately 70% of experimental subjects with acute reflex-induced elevations in BP respond to EA. The most recent grant has focused on a long-loop pathway extending from the hypothalamic arcuate nucleus to the midbrain ventrolateral periaqueductal gray (vlPAG) to the medullary midline nucleus raphe' pallidus (NRP) and then the rostral ventrolateral medulla (rVLM) where the activity of premotor sympathetic neurons is inhibited by EA. We specifically identified a reinforcing reciprocating network between the arcuate and the vlPAG that serves to prolong the action of EA. We also defined an important role for the NRP as a nucleus that processes glutamatergic input from the vlPAG and through a 5-HT1A rVLM mechanism modulates sympathetic outflow. The central hypothesis of this competitive renewal is that a standardized course of acupuncture treatment and established methods convert nonresponders to responders to increase EA's efficacy in reducing elevated blood pressure (BP), particularly through its inhibitory action in the hypothalamic paraventricular nucleus (PVN). The present grant will focus on four new areas: 1) the role of the PVN modulated during EA by opioid (enkephalin and endorphinergic) inputs from the arcuate and through projections to the rVLM and IML inhibits reflex-evoked sympathetic activation, 2) the mechanism through which repetitive EA in conscious cold-induced hypertensive (CI-HTN) rats lowers BP, 3) transcriptional mechanisms regulating enkephalin and endorphin production in the rVLM and arcuate during repetitive stimulation in EA's action to lower BP in CI-HTN rats and 4) cholecystokinin neuromodulation of opioid activity in the PVN of animals unresponsive to the actions of EA. Anatomical, physiological, electrophysiological, pharmacological and molecular approaches will be used in the proposed studies that are highly relevant to patients that have the potential to benefit from clinical acupuncture.

描述（由申请人提供）：我们研究了针灸，特别是电针（EA）调节血压（BP）的机制。 EA具有降低BP升高并减轻需求引起的心肌缺血的能力。它的动作发作缓慢，但其行动时间延长。大约70％的BP反射诱导高程的实验受试者对EA有反应。最新的赠款集中在一条从下丘脑弧形核延伸到中脑腹外侧灰灰色（VLPAG）到髓质中线核核pallidus（NRP），然后是the the the the sotter the Perivestore portive portive portive portive portive portive portive portive，我们明确确定了弧形和VLPAG之间的增强往复网络，该网络可延长EA的作用。我们还将NRP的重要作用定义为一个核，该核从VLPAG和5-HT1A RVLM机制调节交感神经流出。这种竞争性更新的核心假设是，标准化的针灸治疗过程和建立的方法将无反应器转换为响应者，以提高EA在降低血压升高（BP）方面的疗效，尤其是通过其在下丘脑旁脑室中的抑制作用（PVN）。目前的赠款将重点关注四个新领域：1）在EA期间由阿片类药物（Enkephalin和内源性和内源能力）调节的作用，从弧形和通过对RVLM进行投影和IML抑制反射诱发的交感神经激活，2）通过有意识的ea thercection proctiention proctiention proctiational proction hypercation（3）抑制反射性诱导的型号（3），该机制在3个proctiential proctientional proctiency procked（2）中。调节RVLM中的Enkephalin和内啡肽产生的机制，并在EA的作用中重复刺激降低CI-HTN大鼠的BP和4）在动物对EA的作用不反应的动物PVN中的胆囊动蛋白神经调节的重复刺激。解剖学，生理，电生理，药理和分子方法将用于拟议的研究中，这些研究与有可能受益于临床针灸的患者高度相关。