Lab-on-a-chip Flow Cytometer Using COlor-Space-Time (COST) Coding Method

使用颜色时空 (COST) 编码方法的芯片实验室流式细胞仪

基本信息

批准号：
8959759
负责人：
Lawrence S. Goldstein
金额：
$ 1.72万
依托单位：
NANOCELLECT BIOMEDICAL, INC.
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-01 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8959759
关键词：
Address Aerosols Affect Algorithms Alzheimer&apos s Disease Architecture Basic Science Biochemical Biohazardous Substance Biomedical Research Businesses Calibration Cancer Immunology Science Cell Cycle Cell Separation Cell Size Cell Survival Cell surface Cells Clinic Code Color Computer software Conflict (Psychology)Cycloparaffins Detection Devices Disease Evaluation Event Face Feedback Financial compensation Flow Cytometry Fluorescence-Activated Cell Sorting Goals Gold Health Human Immunology Incidence Individual Industry Injection of therapeutic agent Laboratories Laboratory Research Marketing Measures Methods Microfluidic Microchips Mission Molds Neurons Optics Performance Phase Ploidies Polymers Process Production Property Pump Reagent Research Research Personnel Resolution Sampling Schedule Scheme Scientist Shapes Signal Transduction Sorting - Cell Movement Source Staging Stem Cell Research Stem cells System Technology Testing Time Training base cancer stem cell cell injury cell type clinical application commercialization cost design electric impedance evaluation/testing fluorescence activated cell sorter device fluorophore improved induced pluripotent stem cell innovation instrument interest micro-total analysis system neoplastic cell particle physical separation polycarbonate prevent programs prototype public health relevance shear stress software systems system architecture tool user-friendly voltage

项目摘要

DESCRIPTION (provided by applicant): Fluorescence-activated-cell-sorting (FACS) or flow cytometry enables clinicians and researchers to quantitatively characterize the physical (cell size, shape, granularity) and biochemical (DNA content, cell cycle distribution, cell surface markers, and viability) properties of cells. With the capability of high-throughput sorting to enrih biospecimens and extract rare cell types, a state-of-the-art flow cytometer makes it possible to conduct rare-event studies such as the identification or isolation of bacterial cells, stem cells, r tumor cells. However, current flow cytometers that detect multiple colors and sort cells are expensive (~$500K), complicated, hazardous, large and bulky. For these reasons, flow cytometers are often shared amongst labs, leading to conflicts in sample handling and scheduling. With a growing market expected to reach over $3 billion by 2015, flow cytometry can address a diverse array of biomedical challenges. However, there are no high performance flow cytometers with cell sorting capabilities that are affordable for any lab. Despite the increasing demand for affordable cell sorters, the flow cytometry industry faces fundamental limits in the current technology and its evolutionary path. The current system architectures are highly inefficient in accommodating and fully utilizing the increasing number of available fluorescent colors, sensitivity, ease of use, and sorting capabilities. We propose an accessible, affordable, and high performance flow cytometry technology that will allow any scientist to perform cell analysis and sorting in their own laboratory. In this Lab-to-Market Phase II program, we propose to dramatically improve our flow cytometric cell sorting platform, the WOLF Cell Sorter, including extending the dynamic range of the fluorescent detection, implementing real time cell sorting verification, expanding chip volume manufacturing, and validating the system with early adopters such as induced pluripotent stem cell researchers excited by the unique features of our system. These technology and business goals will have a direct impact on basic research and clinical applications in human health and disease.

描述（由申请人提供）：荧光激活细胞分选（FACS）或流式细胞术使临床医生和研究人员能够定量表征物理（细胞大小、形状、粒度）和生化（DNA 含量、细胞周期分布、细胞表面标记）和活力）细胞的特性。最先进的流式细胞仪具有高通量分选能力来丰富生物样本并提取稀有细胞类型，使得进行稀有事件研究成为可能，例如细菌细胞、干细胞、细胞的鉴定或分离。肿瘤细胞。然而，当前检测多种颜色和分选细胞的流式细胞仪价格昂贵（约 50 万美元）、复杂、危险、庞大且笨重。由于这些原因，流式细胞仪通常在实验室之间共享，导致样品处理和安排方面的冲突。随着市场不断增长，预计到 2015 年将达到 30 亿美元以上，流式细胞术可以解决各种生物医学挑战。然而，目前还没有任何实验室都能负担得起的具有细胞分选功能的高性能流式细胞仪。尽管对经济型细胞分选仪的需求不断增加，但流式细胞术行业在当前技术及其发展道路上面临着根本性限制。当前的系统架构在容纳和充分利用越来越多的可用荧光颜色、灵敏度、易用性和分类能力方面效率非常低。我们提出了一种易于使用、价格实惠且高性能的流式细胞术技术，使任何科学家都可以在自己的实验室中进行细胞分析和分类。在这个实验室到市场第二阶段计划中，我们建议大幅改进我们的流式细胞术细胞分选平台，WOLF细胞分选仪，包括扩展荧光检测的动态范围，实施实时细胞分选验证，扩大芯片批量制造，并与早期采用者一起验证该系统，例如对我们系统的独特功能感到兴奋的诱导多能干细胞研究人员。这些技术和商业目标将对人类健康和疾病的基础研究和临床应用产生直接影响。