Invasion and Exclusion by Enterococcus faecalis in the Manduca gut community

粪肠球菌对天蛾肠道群落的入侵与排除

基本信息

批准号：
8775680
负责人：
RONALD R BREAKER
金额：
$ 27.57万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-12-07 至 2015-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8775680
关键词：
Address Affect Animals Antibiotics Bacillus thuringiensis Bacteria Behavior Behavioral Genetics Biochemical Genetics Biological Chemicals Coin Collaborations Colon Carcinoma Communicable Diseases Communities Community Health Complex Core Facility Disease Ecology Enterococcus Enterococcus faecalis Environment Exclusion Genes Genetic Genetic study Genomics Genotype Goals Health High-Throughput Nucleotide Sequencing Human Image Insecta Institutes Intervention Introns Invaded Laboratories Life Link Manduca Manduca sexta Marriage Mass Spectrum Analysis Mental Depression Metabolic Metagenomics Microarray Analysis Microbiology Minnesota Modeling Mutation Nature Nutrient Organism Pathogenesis Pathogenicity Peptides Pharmaceutical Preparations Phenotype Plants Population Portraits Probiotics Process Research Research Personnel Resistance Role Sampling Sequence Analysis Sex Attractants Signaling Molecule Site Small RNA Soil System Systems Theory Technology Universities Work base design differential expression gene discovery genetic analysis innovation luminescence metabolomics microbial microbial community mutant novel pathogen prevent professor response tool transcriptomics

项目摘要

Modulating microbial communities for human health requires understanding the response of communities to invaders, but little is known about the mechanistic bases for community invasion. Our goal is to determine the genetic basis for invasion in the simple model, the gut community of Manduca sexta, and determine the impact of invasion on the meta-metabolome of the host-community complex. We will identify genes involved in invasion and then determine their role in competition and cooperation in the community and their impact on the metabolome to generate hypotheses about the role of signal molecules, antibiotics, and nutrients in community interactions. The project's innovation derives from the marriage of systems-level analysis of metabolite profiles with reductionist genetic analysis, the application of genetics to community-level phenotypes, quantification of microbial populations with luminescence imaging in live animals, and cutting edge chemical technology. The work has the potential to advance the field of microbial community ecology by providing a basis for deriving models about community behavior in the face of perturbation that may apply directly to human health.

为了人类健康调节微生物群落需要了解微生物群落的反应入侵者，但人们对群落入侵的机制基础知之甚少。我们的目标是确定简单模型中天蛾肠道群落入侵的遗传基础，并确定影响对宿主-群落复合体的元代谢组的入侵。我们将鉴定参与入侵的基因，然后确定它们在竞争与合作中的作用群落及其对代谢组的影响，以产生有关信号分子作用的假设，社区互动中的抗生素和营养素。该项目的创新源于代谢物谱的系统级分析与还原论遗传分析，遗传学在群落水平表型中的应用，量化在活体动物中进行发光成像的微生物种群以及尖端化学技术。这这项工作有可能通过提供推导基础来推进微生物群落生态学领域的发展关于面对扰动时的社区行为的模型可能直接适用于人类健康。