Investigation of Epigentic Modifiers in Bladder Cancer

膀胱癌表观修饰因子的研究

• 批准号: 8938212
• 负责人: Piyush Agarwal
• 金额: $ 6.33万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8938212
• 关键词: Aftercare; Biological Assay; Cancer cell line; Cell Line; Cell Proliferation; Cells; Epigenetic Process; Heat-Shock Proteins 90; Histone Deacetylase Inhibitor; Investigation; Malignant neoplasm of urinary bladder; MicroRNAs; Muscle; Mutation; Pharmaceutical Preparations; Proteomics; The Cancer Genome Atlas; Work; inhibitor/antagonist; novel; tumor

1. Treat panel of bladder cancer cell lines with panel of epigenetic modifying drugs (HDAC inhibitors, HNMT inhibitors, etc) 2. Evaluate cell proliferation by MTS assay 3. Identify changes in microRNA pre- and post-treatment 4. Perform proteomic analysis of cells pre and post treatment to see if any other changes are noted that can be modified or manipulated. 5. Investigate synergy with HSP90 inhibitors as these drugs can potentiate modifiers of epigentic machinery. This latter aim may be done with Dr. Neckers

1。用表观遗传修饰的药物（HDAC抑制剂，HNMT抑制剂等）处理膀胱癌细胞系的面板。 5。研究与HSP90抑制剂的协同作用，因为这些药物可以增强表观观念机械的修饰剂。后一个目标可以用Neckers博士完成