Contribution of Y-specific Genes to Bladder Cancer

Y 特异性基因对膀胱癌的影响

• 批准号: 8938210
• 负责人: Piyush Agarwal
• 金额: $ 25.31万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8938210
• 关键词: Androgen Receptor; Biological Assay; Cell Line; Cells; Development; Female; Genes; Growth; Homologous Gene; Immigration; Malignant neoplasm of urinary bladder; Methylation; Play; Predisposition; Role; Woman; Y Chromosome; knock-down; male; men; research study

1. investigate function of USP9Y, DDX3Y, SMCY on growth, proliferation, invasion/migration in bladder cancer through knockdown and over-expression assays 2. investigate mechanism of regulating bladder cancer growth 3. examine functions of female X homologous genes and see if they are similar to these Y-specific genes 4. investigate methylation effects associated with sMCY gene. We are doing these experiments in cell lines derived from men and cells derived from women. The lines derived from women will be investigated further by knocking down the homologous X gene to the Y-specific gene and see if this knockdown behaves similarly in these cell lines as Y-specific knockdown behaves in male-derived cell lines.

1。研究USP9Y，DDX3Y的功能，SMCY对通过敲低和过表达测定法对膀胱癌的增殖，侵袭/迁移进行研究2。研究调节膀胱癌生长的调节机制3。检查雌性X同源基因的功能，并查看与这些Y特异性属性相似的甲基化甲基化4。与Smcy Gene的作用相似。我们正在从源自女性的男性和细胞衍生的细胞系中进行这些实验。通过将同源X基因击倒到Y特异性基因的同源X基因，并查看这种敲低在这些细胞系中的行为是否相似，将进一步研究来自女性的线条。