ImageStream X Mark II for NJMS Flow Core

用于 NJMS Flow Core 的 ImageStream X Mark II

基本信息

批准号：
8640570
负责人：
PATRICIA FITZGERALD-BOCARSLY
金额：
$ 43.95万
依托单位：
RBHS-NEW JERSEY MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-10 至 2015-05-09
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The investigators in this Shared Instrument Grant (SIG) application at New Jersey Medical School request funds for purchase of a state-of-the-art Amnis ImageStreamX Mark II imaging flow cytometer to replace our outdated, first-generation ImageStream 100 that was purchased in late 2007 with an SIG awarded to the PI of the current application. The ImageStreamX Mark II will be housed and administrated through the Flow Cytometry and Immunology Core Laboratory at NJMS, which has strong institutional support from NJMS. Imaging flow cytometry provides the high-throughput content of traditional flow cytometry coupled with the ability to carry-out image analysis on cell populations in flow. This technology allows for analysis, for example, of nuclear translocation, co-localization studies, intracellular pathogen tracking, phagocytosis, FRET, and apoptosis in populations of cells. We are requesting that the ImageStreamX Mark II be equipped with four excitation lasers: 488, 405, 561 and 592nm lasers, plus the standard 785nm SSC laser. In addition, we request that the instrument be equipped with 12 imaging channels, the Multi-Mag option to provide flexible 20X, 40X and 60X imaging capabilities, an extended depth-of-field element, and a 96-well plate auto-sampler. Finally, we request a stand-alone work station for data analysis. The requested ImageStreamX Mark II has multiple improvements over the first generation instrument: most importantly, the ImageStreamX Mark II as requested will allow for up to 10-fluorochomes to be used in a single sample and is up to 20X faster than the original ImageStream 100. The ability to simultaneously evaluate 10 markers in imaging flow dramatically extends the capability of the instrument compared to the four colors with our original instrument and allows investigators to adapt multi-color flow panels used for traditional flow and cell sorting to the ImageStreamX Mark II. These improvements to the instrumentation, along with the Multi-Mag element make the ImageStreamX Mark II a much more versatile instrument that can be used to carry-out sophisticated, multi-color flow cytometric analysis, even on very rare cells. The SIG investigators represent a group of highly-productive scientists from diverse departments at the New Jersey Medical School, almost all of whom have NIH R01 funding. Many of the SIG investigators have effectively utilized our existing ImageStream 100 and have published papers and obtained grants with ImageStream data, but have found the need for more sophisticated imaging flow cytometry. There are 8 major and 5 major users for this SIG application. While many are immunologists, the SIG investigators also include a neuroscientist, a cell physiologist, a microbiologist and biochemists. Thus, the ImageStreamX Mark II is expected to provide valuable imaging flow cytometry capabilities across a wide diversity of disciplines. This instrument will enhance the productivity of the SIG investigators and will also provide valuable opportunities for pre- and post-doctoral trainees.

描述（由申请人提供）：新泽西医学院的共享仪器赠款（SIG）申请中的调查人员要求购买最先进的Amnis Imagestreamx Mark II成像流式细胞仪，以取代我们在2007年末购买的过时的，第一代ImaTealeam 100，并通过2007年底购买了授予当前应用程序的SIG。 ImageStreamx Mark II将通过NJMS的流式细胞仪和免疫学核心实验室来容纳和管理，该实验室具有NJMS的大力机构支持。成像流式细胞仪提供了传统流式细胞仪的高通量含量，并能够对流量中的细胞群体进行图像分析的能力。该技术允许分析细胞种群中核易位，共定位研究，细胞内病原体跟踪，吞噬作用，fret和凋亡。我们要求Imagestreamx Mark II配备四个激发激光器：488、405、561和592NM激光器，以及标准的785nm SSC激光器。此外，我们要求该仪器配备12个成像通道，可提供灵活的20倍，40倍和60x成像功能，扩展的视野元素以及96孔板自动采样器的多MAG选项。最后，我们要求一个独立的工作站进行数据分析。所需的ImageStreamx Mark II比第一代工具具有多次改进：最重要的是，按要求的ImageStreamx Mark II允许在单个样本中使用多达10个荧光色素，并且比原始的ImageStream 100更快地使用20倍。与Implanter and Ordection相比，与原始仪器相比，与原始仪器相比，与四色的功能相比，我们的四个颜色相比，我们的功能与四个颜色相比，我们的功能是相比的。用于传统流量和细胞分类的流动面板与Imagestreamx Mark II。这些对仪器的改进以及多MAG元件以及Imagestreamx Mark II的多功能仪器，即使在非常罕见的细胞上也可以用于进行复杂的多色流式细胞术分析。 SIG调查人员代表了来自新泽西医学院不同部门的一群高产科学家，几乎所有人都有NIH R01资金。许多SIG研究者有效地利用了我们现有的成像讲100，并发表了文章并获得了具有成像级数据的赠款，但发现需要更复杂的成像流式细胞仪。此SIG应用程序有8个主要用户和5个主要用户。尽管许多是免疫学家，但SIG研究人员还包括神经科学家，细胞生理学家，微生物学家和生物化学家。因此，预期Imagestreamx Mark II将在广泛的学科中提供有价值的成像流式细胞仪能力。该工具将提高SIG调查人员的生产率，还将为学前和博士后学员提供宝贵的机会。