Amnis ImageStream Cell Analysis System

Amnis ImageStream 细胞分析系统

基本信息

批准号：
7217807
负责人：
PATRICIA FITZGERALD-BOCARSLY
金额：
$ 35.5万
依托单位：
UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-06-07 至 2008-06-06
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7217807
关键词：
Adjuvant Advisory Committees Anthrax disease Antiviral Response Apoptosis Applications Grants Bone Marrow Cell Communication Cell Cycle Cells Chemotherapy-Oncologic Procedure Color Computer software Consultations Core Facility Cytokine Gene DNA Binding DNA Repair Defect Dendritic Cells Discipline Equipment Faculty Fanconi&apos s Anemia Flow Cytometry Fluorescence Fluorescent in Situ Hybridization Funding Gene Expression HIV Housing Human Herpesvirus 8 Image Imagery Immune response Immune system Immunity Individual Interferon Type II Interferons Kinetics Licensing Lytic Mediating Mesenchymal Stem Cells Mitosis New Jersey Nuclear Translocation Participant Pathogenesis Population Principal Investigator Proteins Purinergic P1 Receptors Radioactivity Research Personnel Research Project Grants Resolution Role Science Scientist Signal Transduction Systems Analysis Technology Time Toll-like receptors Tuberculosis Vaccines Vitamin D Analog angiogenesis autoreactive T cell cytokine data management digital imaging experience in vivo instrument malignant breast neoplasm medical schools melanoma trafficking

项目摘要

DESCRIPTION (provided by applicant): This Shared Instrument Grant proposal is for the purchase of an Amnis ImageStream instrument that combines the power of flow cytometry with the capture of high resolution digital images of cells in flow. The ImageStream is able to detect four colors of fluorescence as well as capture brightfield and darkfield imagery of each cell. The ImageStream does not replace any existing flow cytometric or imaging equipment, but rather, provides a complementary technology that will greatly facilitate the research of the grant participants in applications such as nuclear translocation studies in mixed populations of cells, apoptosis, cell cycle and mitosis studies, marker co-localization, intracellular trafficking, cell:cell interactions, fluorescent in situ hybridization and gene expression/co-localization. In addition to the instrument, a stabilizing table for the ImageStream is also requested. A Dell Workstation that provides powerful data management is requested, as is a multi-user software license for the IDEAS software. The new ImageStream will be housed in the Flow Cytometry Core Facility at the New Jersey Medical School and will be operated and maintained by an experienced flow cytometry technician Ms. Angelika Batista. The Principal Investigator, Dr. Patricia Fitzgerald-Bocarsly will provide administrative and scientific oversight in consultation with a Faculty Advisory Committee. The ImageStream will be primarily utilized by the applicant group of thirteen highly-productive scientists from diverse departments and biomedical science disciplines at the NJMS. They include: 1) Azzam, Edouard - Damage signaling from irradiated to non-irradiated cells; 2) Fitzgerald-Bocarsly, Patricia - Plasmacytoid dendritic cells in HIV pathogenesis; 3) Gause, William - Cytokine gene expression during in vivo immune response; 4) Howell, Roger - Effects of nonuniform distributions of radioactivity; 5) Kotenko, Sergei - Role of interferon lambdas in antiviral response; 6) Lambert, Muriel - DNA repair defect in Fanconi Anemia Group A; 7) Leibovich, Joseph - Toll-like receptors, adenosine and angiogenesis; 8) Lukac, David - DNA binding mechanism of the KSHV lytic switch protein and kinetics of KSHV gene expression; 9) Rameschwar, Pranela - Vaccine hurdle to anthrax and the emerging immune system; IFN-gamma in the neuroimmune-mesenchymal stem cell axis; and Breast cancer-bone marrow stromal interactions; 10) Rohowsky-Kochan, Christine - Cytokine secretion profile of autoreactive T cells; 11) Salgame, Padmini - Induction of Th1 immunity in tuberculosis; 12) Studzinski, George - Vitamin D analogs as adjuvants in chemotherapy of cancer; and 13) Molina, Carlos - Ras-Mediated proteasomal degradation of ICER in melanomas. This group of investigators is anticipated to utilize almost 100% of the total usage of the instrument. Remaining instrument time will be allocated to NIH-funded investigators or other individuals at the Newark campus. Instrument time may be made available to non-UMDNJ investigators from time-to-time when the ImageStream is not otherwise being used.

描述（由申请人提供）：此共享的仪器赠款提案用于购买AMNIS成像仪器，该仪器将流式细胞术的力量与捕获流量中的高分辨率数字图像相结合。图像讲言能够检测四种荧光的颜色，并捕获每个单元的Brightfield和Darkfield图像。 The ImageStream does not replace any existing flow cytometric or imaging equipment, but rather, provides a complementary technology that will greatly facilitate the research of the grant participants in applications such as nuclear translocation studies in mixed populations of cells, apoptosis, cell cycle and mitosis studies, marker co-localization, intracellular trafficking, cell:cell interactions, fluorescent in situ hybridization and gene expression/co-localization.除该仪器外，还请求一张成像流的稳定表。请求提供强大数据管理的Dell Workstation，以及用于Ideas软件的多用户软件许可证。新的成像级式将安置在新泽西医学院的流式细胞仪核心设施中，并将由经验丰富的流式细胞仪技术员安吉利卡·巴蒂斯塔（Angelika Batista）进行操作和维护。首席研究员Patricia Fitzgerald-Bocarsly博士将在与教职员工咨询委员会协商时提供行政和科学监督。 ImageStream将主要由来自NJM的不同部门和生物医学科学学科的13位高度生产性科学家的申请人组利用。它们包括：1）Azzam，Edouard-从辐照到非辐照细胞的损伤信号传导； 2）在HIV发病机理中，Fitzgerald -Bocars，Patricia -Patricia -parmacytoid Dendritic细胞； 3）盖斯，威廉 - 体内免疫反应期间的细胞因子基因表达； 4）Howell，Roger-放射性分布的效果； 5）Kotenko，Sergei-干扰素Lambdas在抗病毒反应中的作用； 6）兰伯特，穆里尔 - 芬科尼贫血A组的DNA修复缺陷； 7）莱博维奇（Joseph） - toll样受体，腺苷和血管生成； 8）Lukac，David -KSHV裂解开关蛋白的DNA结合机理和KSHV基因表达的动力学； 9）Rameschwar，Pranela-疫苗到炭疽和新兴免疫系统；神经免疫 - 间充质干细胞轴中的IFN-gamma；和乳腺癌骨髓的基质相互作用； 10）Rohowsky -Kochan，克里斯汀 - 自动反应性T细胞的细胞因子分泌谱； 11）Salgame，Padmini-诱导结核病的Th1免疫力； 12）Studzinski，George-癌症化学疗法中的维生素D类似物作为佐剂；和13）Molina，Carlos-黑色素瘤中ICER的RAS介导的蛋白酶体降解。预计这组研究人员将利用该工具总用途的几乎100％。剩余的仪器时间将分配给Newark校园的NIH资助的调查人员或其他人。如果不使用成像讲仪，可以不时地使用仪器时间向非UMDNJ调查人员提供。