Skin Cell Culture Core

皮肤细胞培养核心

• 批准号: 8538748
• 负责人: Laura Timares
• 金额: $ 12.7万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8538748
• 关键词: Biology; Biopsy; Biopsy Specimen; Cell Culture System; Cell Culture Techniques; Cells; Communities; Cutaneous; Dermal; Dermatologic; Disease; Ensure; Faculty; Generations; Human; Interdisciplinary Study; Lead; Maintenance; Measures; Methods; Mission; Mus; Mutant Strains Mice; Patients; Protocols documentation; Quality Control; Research; Research Personnel; Research Technics; Resources; Services; Skin; Skin Tissue; Source; Training; cell type; cost; cost efficient; human tissue; improved; innovation; keratinocyte; member; monolayer; skin disorder

As part of the DAB Skin Diseases Research Center (SDRC), the mission of the DAB SDRC Skin Cell Culture Core (SCCC) is to provide valuable resources, services, and training in research techniques for promoting interdisciplinary research in cutaneous biology. Over the last 4 years, our core services have become a vital resource to a diverse community of 35 UAB SDRC members and 12 new investigators representing 22 departments who depend upon our expertise to facilitate and support studies relating to cutaneous biology. The SCCC faculty and research staff have established expertise in the generation and maintenance of purified epidermal and dermal primary cell subsets, derived primarily from murine and human skin tissues or biopsy samples, in monolayer or organotypic cultures. We propose to continue to support and promote cutaneous research at UAB with the following specific aims: 1) To provide a central source of epidermal and dermal cell types from murine and human tissues to Center members in a cost efficient manner. 2) To maintain quality control measures that ensure the distribution of standardized contamination-free cultures. 3) To provide expertise in generating specialized cell culture systems and transfecting skin cells from either human or mouse sources. 4) To optimize methods for continuous long-term culture and immortalization protocols of murine keratinocytes derived from wild type and mutant mouse strains to accommodate increased demand by SDRC members. By providing various cell types at a cost that encourages new exploration and innovation, the SDRC SCCC has been essential for enhancing the quality and expanding the breadth of cutaneous research performed by multiple UAB investigators during last 4 years. We propose to continue and improve upon this vital service.

作为 DAB 皮肤疾病研究中心 (SDRC) 的一部分，DAB SDRC 皮肤细胞培养的使命 核心（SCCC）是提供宝贵的资源、服务和研究技术培训，以促进 皮肤生物学的跨学科研究。在过去 4 年里，我们的核心服务已成为至关重要的 向由 35 名 UAB SDRC 成员和代表 22 个国家的 12 名新调查员组成的多元化社区提供资源 依靠我们的专业知识来促进和支持皮肤生物学相关研究的部门。 SCCC 的教师和研究人员在生成和维护 纯化的表皮和真皮原代细胞亚群，主要源自小鼠和人类皮肤组织或 单层或器官型培养物中的活检样本。我们建议继续支持和推动 UAB 的皮肤研究具有以下具体目标： 1) 为中心提供来自小鼠和人体组织的表皮和真皮细胞类型的中心来源 会员以具有成本效益的方式。 2) 维持质量控制措施，确保配送标准化无污染 文化。 3) 提供生成专门细胞培养系统和转染皮肤细胞的专业知识 人类或小鼠来源。 4) 优化小鼠连续长期培养和永生化方案的方法 来自野生型和突变型小鼠品系的角质形成细胞，以满足不断增长的需求 国家发展改革委成员。 通过以鼓励新探索和创新的成本提供各种细胞类型，SDRC SCCC 对于提高皮肤研究的质量和扩大皮肤研究的广度至关重要 过去 4 年中有多位 UAB 研究人员。我们建议继续并改进这项重要的服务。