Skin Cell Culture

皮肤细胞培养

• 批准号: 7658255
• 负责人: Laura Timares
• 金额: $ 13.11万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7658255
• 关键词: Area; Biology; Cell Culture System; Cell Line; Cells; Cellular biology; Coculture Techniques; Complex; Cultured Cells; Dendritic Cells; Dermal; Development; Endothelial Cells; Ensure; Fostering; Generations; Human; Human Resources; In Vitro; Interdisciplinary Study; Langerhans cell; Maintenance; Measures; Methods; Mus; Nature; Physiological; Primary Cell Cultures; Quality Control; Research; Research Personnel; Services; Skin; Skin Tissue; Source; Tissues; Training; Transfection; Work; cell type; cost effective; in vivo; interest; keratinocyte; melanocyte; member; research study; skin disorder

DESCRIPTION (provided by applicant): The Skin Cell Culture Core (SCCC) aims to promote interdisciplinary research in skin cell biology among UAB campus-wide Skin Diseases Research Center (SDRC) members. The SCCC will provide expertise in the generation and maintenance of epidermal and dermal cell specific cultures from either mouse or human skin. In addition, it will provide expertise in the development of RAFT skin equivalent cultures. The specific aims of the core are l) to provide a central source of epidermal and dermal cell types to Center members in an efficient cost effective manner, 2) to maintain quality control measures to ensure contamination-free cultures, 3) to provide expertise in specialized cell culture systems and transfection for both human and mouse cells and 4) to develop methods for generating mixed co-cultures of raft keratinocytes with Langerhans cells and/or melanocytes to anticipate increased interest and demand by SDRC members.

描述（由申请人提供）：皮肤细胞培养核心（SCCC）旨在促进 UAB 全校皮肤疾病研究中心 (SDRC) 成员进行皮肤细胞生物学的跨学科研究。 SCCC 将提供从小鼠或人类皮肤中生成和维持表皮和真皮细胞特异性培养物的专业知识。此外，它将提供 RAFT 皮肤等效培养物开发方面的专业知识。该核心的具体目标是：l) 以高效、成本有效的方式向中心成员提供表皮和真皮细胞类型的中央来源，2) 维持质量控制措施，确保无污染培养物，3) 提供以下方面的专业知识：专门的细胞培养系统和人类和小鼠细胞的转染，4) 开发筏角质形成细胞与朗格汉斯细胞和/或黑素细胞混合共培养的方法，以预测 SDRC 成员日益增长的兴趣和需求。