Real-time prediction of thermal ablation-induced cell death by echo decorrelation

通过回波去相关实时预测热消融诱导的细胞死亡

基本信息

批准号：
8528513
负责人：
T Douglas Mast
金额：
$ 40.83万
依托单位：
UNIVERSITY OF CINCINNATI
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-08-10 至 2016-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8528513
关键词：
Ablation Accounting Adherence Apoptosis Archives Cancer Patient Carcinoma Categories Cattle Cell Death Cell Survival Cells Cessation of life Clinic Clinical Coagulation Process Coagulative necrosis Custom Data Development Devices Elements Ensure Evaluation Excision Feedback Focused Ultrasound Therapy Future Goals Heating Hematoxylin and Eosin Staining Method Histologic Histology Image Image Analysis Implant In Vitro Link Liver Location Malignant Neoplasms Malignant neoplasm of liver Maps Measures Methods Monitor Morphology Nuclear Oryctolagus cuniculus Outcome Patients Physiologic pulse Plant Roots Procedures Public Health Publishing Radiofrequency Interstitial Ablation Receiver Operating Characteristics Recurrence Research Research Personnel Resolution Safety Series Soft Tissue Neoplasms Staining method Stains TdT-Mediated dUTP Nick End Labeling Assay Testing Thermal Ablation Therapy Time Tissue Viability Tissues Translations Transplantation Treatment Efficacy Tumor Tissue Ultrasonography base cancer therapy improved in vivo indexing interest microwave ablation millisecond minimally invasive mortality novel novel strategies prospective radiofrequency research clinical testing research study success time use treatment planning tumor

项目摘要

DESCRIPTION (provided by applicant):The major goal of this project is to predict thermal ablation-induced cell death in real time using ultrasound echo decorrelation imaging, a method for mapping rapid, ablation-induced changes in tissue over millisecond time scales. Preliminary research has shown a strong correlation between local echo decorrelation and thermal tissue coagulation. However, a direct link between local echo decorrelation and the desired clinical end effect, i.e., death of malignant tumor tissue, has not been established. We hypothesize that ultrasound echo decorrelation imaging can directly predict thermal ablation-induced cell death in real time. Our hypothesis will be tested using a novel ablation and imaging configuration with great advantages for precise, quantitative comparison of treatment plans, ultrasound images, and ablated tissue histology. Image- guided treatments will be performed using an image-treat ultrasound array capable of both thermal ablation and high-quality pulse-echo imaging, using the same piezoelectric array elements. As a result, all imaging and treatment takes place in the same plane, and image-based predictions of local ablation-induced cell death can be evaluated with high accuracy. A custom image-treat ultrasound array will perform ablation and imaging of rabbit liver with implanted VX2 carcinoma in a series of in vivo trials. Ultrasound exposure conditions will include both bulk thermal ablation (comparable to radiofrequency and microwave ablation, as currently used in the clinic for minimally-invasive cancer treatment), and high-intensity focused ultrasound ablation (comparable to noninvasive focused ultrasound ablation devices currently under development and clinical testing for cancer treatment). Experiments will initially test the capability of cumulative echo decorrelation images to predict ablation-induced cell death, as evaluated by vital and histologic staining, with prediction accuracy judged from pixel-by-pixel receiver-operating-characteristic curve analysis. The ability of echo decorrelation imaging to predict coagulative necrosis, partial tissue viability, and heat-induced apoptosis will be statistically assessed. Echo decorrelation will be correlated with local cell viability, mapped by quantitative image analysis of cell nuclear morphology. Further experiments will evaluate a procedure for real-time ablation control, in which treatments are continued until the spatially averaged echo decorrelation exceeds a prespecified threshold within a targeted region of interest. Accuracy of echo decorrelation imaging-controlled will be assessed, both for accurate prediction of cell death in the targeted ROI and for correspondence to the targeted ablation margins. If successful, this project will provide clinicians with a real-time, ultrasound-based imaging approach to predict thermal ablation-induced cell death in real time during thermal ablation. This new opportunity for real-time monitoring and control of thermal ablation procedures will ultimately result in greatly improved efficacy and safety for these clinically important, minimally invasive and noninvasive cancer treatments.

描述（由申请人提供）：该项目的主要目标是使用超声回声去相关成像实时预测热消融诱导的细胞死亡，这是一种在毫秒时尺度上绘制快速，消融诱导的组织变化的方法。初步研究表明，局部回声去相关与热组织凝结之间存在很强的相关性。但是，尚未建立局部回声去相关与所需的临床终端效应（即恶性肿瘤组织死亡）之间的直接联系。我们假设超声回声去相关成像可以直接预测热消融诱导的细胞死亡。我们的假设将使用新颖的消融和成像配置进行检验，具有很大的优势，可用于对治疗计划，超声图像和消融组织学的精确定量比较。图像引导处理将使用能够使用相同的压电阵列元件的图像处理超声阵列进行热消融和高质量的脉搏回声成像。结果，所有成像和处理都在同一平面上进行，并且可以高精度评估局部消融诱导的细胞死亡的基于图像的预测。自定义图像处理的超声阵列将在一系列体内试验中用植入的VX2癌对兔肝脏进行消融和成像。超声暴露条件将包括散装热消融（与当前在最小侵入性癌症治疗的诊所中使用的辐射频率和微波消融相当），以及在开发和临床治疗中进行的无创焦点超声消融（可与非侵入性的聚焦超声消融设备相当）。实验最初将测试累积回声去相关图像的能力，以预测消融诱导的细胞死亡，通过生命和组织学染色评估，并从像素逐像素接收器 - 操作 - 操作 - 特征特性曲线分析中进行了预测准确性。回声去相关成像预测凝血坏死，部分组织活力和热诱导的凋亡的能力将得到统计评估。回声去相关将与局部细胞活力相关，并通过细胞核形态的定量图像分析映射。进一步的实验将评估一种实时消融控制的程序，在该程序中继续进行处理，直到空间平均的回声去相关超过了目标感兴趣的目标区域内的预先指定的阈值。将评估回声去相关成像对照的准确性，既要精确预测靶向ROI中的细胞死亡，又可以评估与靶向消融边缘的对应关系。如果成功的话，该项目将为临床医生提供一种基于超声的成像方法，以预测热消融过程中实时的热消融诱导的细胞死亡。实时监测和控制热消融程序的新机会最终将极大地提高这些临床重要，微创和无创癌症治疗的功效和安全性。