Lectimers: Glycan-anchored scaffold libraries for targeting carbohydrate-binding

Lectimers：用于靶向碳水化合物结合的聚糖锚定支架文库

基本信息

批准号：
8906828
负责人：
MATTHEW LEVY
金额：
$ 21.79万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-20 至 2017-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8906828
关键词：
Affinity Avidity Binding Biological Markers Biological Process Carbohydrates Cells Chemicals DNA DNA biosynthesis Development Diagnostic Disaccharides Disease Ensure Galactose Binding Lectin Galectin 1 Galectin 3 Health Human Hydroxyl Radical Individual Lectin Libraries Ligands Malignant Neoplasms Methodology Methods Modification Monosaccharides Nucleic Acids Peptides Phase Play Polymerase Polysaccharides Positioning Attribute Protein Binding Protein Family Proteins Reading Reagent Role Solid Specificity System Technology Therapeutic Therapeutic Human Experimentation Time Ursidae Family Variant Vertebral column Virus Diseases base cancer type carbohydrate binding protein chemical synthesis cost flexibility fluorophore functional group human disease immunoregulation member monomer next generation sequencing novel phosphoramidite population based scaffold small molecule statistics sugar tumorigenesis

项目摘要

DESCRIPTION (provided by applicant): We propose to develop a novel platform technology that will produce high-affinity and high-specificity ligands to target carbohydrate binding protein (CBPs), a diverse class of proteins which possess a wide range of biological functions, playing roles cancer tumorigeneisis, immune modulation and viral infection. Our method builds upon advances in sequencing technologies and leverages the natural, low affinity, interactions of monovalent sugars for CBPs combined with the ease of synthesis of nucleic acids. Our glycan-anchored libraries will thus possess many of the attributes of nucleic acids - in particular structural rigidity, ease of synthesis and the ease with which they can be amplified and characterized - but with the enhanced chemical functionality observed in peptides, proteins and small molecule ligands. Because these reagents are based on nucleic acids, the resulting affinity agents can be readily synthesized at relatively low cost and can be easily modified with a variety of fluorophores or chemical moieties for diagnostic, therapeutic and research purposes.

描述（由申请人提供）：我们建议开发一种新型的平台技术，该技术将产生高亲和力和高特异性配体，以靶向碳水化合物结合蛋白（CBP），这是一种多样化的蛋白质，具有广泛的生物学功能，具有广泛的生物学功能，扮演癌症癌症，免疫调节和病毒感染。我们的方法基于测序技术的进步并利用自然，低亲和力，单价糖的CBP相互作用，结合了核酸的合成易于性。因此，我们的聚糖锚定文库将拥有许多核酸的属性 - 特别是结构刚性，易于合成以及它们可以放大和表征的易于性 - 但在肽，蛋白质和小分子配体中观察到的化学功能增强。由于这些试剂基于核酸，因此所产生的亲和力可以很容易地以相对较低的成本合成，并且可以轻松地通过A 用于诊断，治疗和研究目的的各种荧光团或化学部分。