Development of SFS Fractionators

SFS分馏塔的开发

• 批准号: 8712743
• 负责人: TREVOR P. CASTOR
• 金额: $ 22.49万
• 依托单位: APHIOS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2014-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8712743
• 关键词: Animals; Biological Assay; Biological Factors; Biomass; Biotechnology; Carbon Dioxide; Castor; Collection; Complex Mixtures; Development; Drug Industry; Emotional; Equipment; Erinaceidae; Ethanol; Exhibits; Food; Fractionation; Gases; Generations; Genes; Ginger; Goals; Health; Health Benefit; Hour; Human; Hypericum perforatum; Legal patent; Libraries; Licensing; Lilium; Liquid substance; Marines; Masks; Medicine; Methanol; Microbe; Modality; Molecular; National Center for Complementary and Alternative Medicine; Natural Products Chemistry; Nausea and Vomiting; Needles; Noise; Nutraceutical; Organic solvent product; Oxidoreductase; Paclitaxel; Pathway interactions; Penetration; Personal Satisfaction; Pharmaceutical Preparations; Pharmacologic Substance; Phase; Phase III Clinical Trials; Plants; Population; Powder dose form; Probability; Process; Property; Prostate; Protocols documentation; Recovery; Research; Research Institute; Research Methodology; Research Personnel; Research Project Grants; Resources; Sabal serrulata; Sampling; Serotonin; Site; Small Business Innovation Research Grant; Software Design; Solid; Solvents; Source; Structure; Taxoids; Taxus; Technology; Testing; Therapeutic; Thermodynamics; Time; Traditional Medicine; Trees; Universities; Veratrum; World Health Organization; base; bryostatin; cost; cost effective; cyclopamine; design; dietary supplements; drug discovery; hyperforin; improved; method development; microorganism; parallel processing; product development; programs; prototype; public health relevance; research and development; reuptake; screening; shogaol; user-friendly

DESCRIPTION (provided by applicant): The screening of a complex mixture of analytes is not always successful in sensitive screens for specific activities. Often, specific activities are masked by interfering compounds or false positives are given by ubiquitous compounds that interact with all screens. This problem can be resolved by purification of the analytes. Such an approach is not only cost-prohibitive but will also result in biasing the isolation of analytes tha occur in higher concentrations. Conventional organic solvents are not always ideal for biomass extractions. These solvents can be difficult to remove from the compounds potentially exhibiting bioactivity and, thus can mask bioactivity in an assay. In order to enhance the drug and dietary supplement discovery process, we have developed and patented processes for the polarity-guided fractionation of plants and microbes (Castor et al., 1998, 2003) using supercritical fluid solvents. The overall goal of this research project is to develop a commercial, user-friendly SuperFluidsTM CXF fractionator that can be utilized by researchers at universities and pharmaceutical companies to facilitate the drug discovery and nutraceutical product development process. The primary Phase I object is to design a parallel processing CXF fractionator, and to construct and test a single unit CXF prototype that can process samples in accordance to a pre-defined sequentially increasing-polarity protocol. The unit will have the capability to rapidly produce partially purified natural product molecules from terrestrial plants and marine microorganisms, for example, 6 varying polarity fractions in about 1 hour. In Phase I, we plan to design SuperFluidsTM CXF prototypes, construct key components of the prototypes, and test these components. In Phase II, we plan to complete the hardware and software design of the SuperFluidsTM fractionation unit, assemble single and parallel processing Alpha-site units, test the units at a Beta-site, and redesign and reconstruct as necessary to eliminate bugs and increase user-friendliness. In Phase III, OEM companies will manufacture commercial units. In Phase III, we plan to sell SFS fractionation units, which will include license to utilize the SFS-CXF technologies and equipment, to researchers and product developers at universities as well as nutraceutical, food, pharmaceutical and biotechnology companies.

描述（由申请人提供）：在特定活性的敏感筛选中，复杂的分析物混合物的筛选并不总是成功。通常，特定的活动会被干扰化合物掩盖，或者与所有屏幕相互作用的普遍存在的化合物会产生误报。这个问题可以通过分析物的纯化来解决。这种方法不仅成本高昂，而且还会导致高浓度分析物的分离出现偏差。传统的有机溶剂并不总是适合生物质提取。这些溶剂可能很难从可能表现出生物活性的化合物中去除，因此可能会掩盖测定中的生物活性。为了加强药物和膳食补充剂的发现过程，我们开发了使用超临界流体溶剂对植物和微生物进行极性引导分馏的工艺并申请了专利（Castor 等人，1998 年，2003 年）。该研究项目的总体目标是开发一种商业化、用户友好的 SuperFluidsTM CXF 分馏器，可供大学和制药公司的研究人员使用，以促进药物发现和营养保健品开发过程。第一阶段的主要目标是设计并行处理 CXF 分馏器，并构建和测试单个单元 CXF 原型，该原型可以根据预定义的顺序递增极性协议处理样品。该装置将能够从陆地植物和海洋微生物中快速生产部分纯化的天然产物分子，例如在大约 1 小时内生产 6 种不同极性的组分。在第一阶段，我们计划设计SuperFluidsTM CXF原型，构建原型的关键组件，并测试这些组件。在第二阶段，我们计划完成SuperFluidsTM分馏装置的硬件和软件设计，组装单个和并行处理的Alpha站点单元，在Beta站点测试单元，并根据需要重新设计和重建，以消除错误并增加用户-友善。在第三阶段，OEM 公司将生产商业装置。在第三阶段，我们计划向大学以及营养保健品、食品、制药和生物技术公司的研究人员和产品开发人员出售 SFS 分馏装置，其中包括使用 SFS-CXF 技术和设备的许可。