Virus Core

病毒核心

• 批准号: 8580825
• 负责人: Randy A. Albrecht
• 金额: $ 10.99万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8580825
• 关键词: Communication; Data; Development; Genes; Genetic Techniques; Hemagglutination; Human Resources; Immune response; In Vitro; Influenza; Instruction; Maintenance; Mediating; Microbiology; Modeling; Mutation; Phase; Plaque Assay; Post-Translational Protein Processing; Preparation; Procedures; Production; Recombinants; Resources; Therapeutic Intervention; Training; Variant; Viral; Viral Pathogenesis; Viral Proteins; Virus; Virus Replication; Work; base; cost effective; in vivo; influenzavirus; medical schools; pathogen; positional cloning; protein protein interaction; recombinant virus; research study; virus core; virus pathogenesis

PROJECT SUMMARY (See instructions): A. Rationale The Virus Core is an essential resource for the needs of projects 1 and 2, which will benefit from a centralized Virus Core by: i) the established expertise with reverse genetics techniques which will facilitate rescue of recombinant influenza viruses that are described below and itemized in Table 1B, ii) the maintenance of influenza virus stocks that have been sequence-confirmed and assessed for quality by hemagglutination and plaque assays, and iii) reduced inter-experimental variation by consistent use of specific virus stock preparations. The Department of Microbiology, Mount Sinai School of Medicine, NY, NY is a pioneer in the application of reverse genetics and the development of recombinant viruses. The well equipped facilities, established procedures, and properly trained personnel provide a cost-effective Virus Core that will result in efficient production of wild-type and recombinant influenza virus stocks that will be essential for projects 1 and 2. Specifically, Dr. Megan Shaw (Co-PI of project 1) and Dr. Adolfo Garcia-Sastre (Co-PI of project 2) will directly benefit by their close proximity to and direct communication with the Virus Core. B, Specific functions of the Virus Core 1) Maintain working stocks of wild-type influenza viruses for use by projects 1 and 2. 2) Generate recombinant influenza viruses for use by projects 1 and 2.

项目摘要（参见说明）： A. 基本原理 病毒核心是满足项目 1 和 2 需求的重要资源，它将通过以下方式受益于集中式病毒核心： i) 反向遗传学技术的既定专业知识，这将有助于拯救重组流感病毒，如下所述并逐项列出表 1B，ii) 已通过血凝和空斑测定进行序列确认和质量评估的流感病毒母液的维持，以及 iii) 通过一致使用特定病毒母液制剂减少实验间变异。纽约州西奈山医学院微生物学系是反向遗传学应用和重组病毒开发的先驱。装备精良的设施、既定的程序和经过适当培训的人员提供了具有成本效益的病毒核心，这将导致野生型和重组流感病毒库存的高效生产，这对于项目 1 和 2 至关重要。具体来说，Megan Shaw 博士（项目 1 的联合 PI）和 Adolfo Garcia-Sastre 博士（项目 2 的联合 PI）将直接受益于他们与病毒核心的密切接触和直接通信。 B、病毒核心的具体功能 1) 维持野生型流感病毒的工作库存，供项目 1 和 2 使用。 2) 生成重组流感病毒供项目1和2使用。