Cellular Neuroscience Core

细胞神经科学核心

• 批准号: 8723675
• 负责人: DOUGLAS A COULTER
• 金额: $ 16.73万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2015-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8723675
• 关键词: Animals; Biochemistry; Brain; Cell Culture Techniques; Cell physiology; Cells; Childhood; Color; Counseling; Data; Development; Disease; Embryo; Ensure; Functional disorder; Funding; Goals; Human; Human Resources; Ice; Image; In Situ Hybridization; Institution; Leadership; Life; Mental Retardation and Developmental Disabilities Research Centers; Methods; Microscope; Microscopy; Mitochondria; Molecular; Neurons; Neurosciences; Pediatric Hospitals; Physiological; Policies; Preparation; Productivity; Proteins; Reagent; Research; Research Personnel; Series; Specimen; Staging; Stem cells; Surveys; Techniques; Technology; developmental disease; disability; experience; innovation; intellectual and developmental disability; interest; micromanipulator; neuropathology; novel; pleasure; response; skills

The initial (1999) application for this IDDRC included separate cores in Neuropathology and Neuroscience, with the latter offering expertise in cell culture. As user needs evolved, it became apparent that these facilities should be merged into a single Cellular Neuroscience Core. This was accomplished in 1994 with Dr. Pleasure as the Director. He served in this capacity unfil 2001, when leadership passed to Dr. Jeffrey Golden, a pediatric neuropathologist with a primary interest in developmental disorders. Dr. Golden first joined CHOP in 1997 and immediately became engaged with the IDDRC, both as a user and as an Associate Director of the Cellular Neuroscience Core. Since its inception this Core has provided users with a diverse repertoire of state-of-the-art methods for visualizaion of the distribufions of gene products in normal, developing neural cells and in those undergoing various forms of degeneration and regenerafion. We have confinually and eagerly added new skills, instrumentafion and reagents to better serve our users' needs. In 1995, with the generous assistance of the Children's Hospital, we purchased a Leica confocal microscope to which we added inverted microscopy, stagemounted micromanipulators/microinjectors, and a stage-mounted environmental chamber, thus permitting prolonged observation and manipulation of living cells under physiological conditions. In this manner we offered 8-color capacity fluorescent imaging. Our institution paid for the apparatus and we used IDDRC funding to partially support a technician. We made several other notable technological additions to the Core repertoire, including in situ hybridization in both sections and whole embryos. Our general purpose has been not only to make available a technology, but a consultative sen/ice that facilitates implementation of the method as well as the interpretation of data. We adhered to this policy when we also added video-enhanced microscopy in order to better support IDDRC investigators in analyzing intracellular Ca2+ and Na+ as well as the estimate of mitochondrial potential.

该IDDRC的最初（1999年）应用包括神经病理学和神经科学中的单独核心，后者在细胞培养中提供了专业知识。随着用户需求的发展，显然应该将这些设施合并为单个细胞神经科学核心。这是在1994年以Pleasure博士担任董事而实现的。他以这种身份为2001年，当时领导层转移给了对发育障碍的主要兴趣的儿科神经病理学家杰弗里·戈登（Jeffrey Golden）博士。 Golden博士于1997年首次加入Chop， 作为用户和蜂窝神经科学核心的副主任，立即与IDDRC互动。 自成立以来，该核心为用户提供了各种各样的最新方法，用于可视化基因产物的分布在正常的神经细胞中，以及在各种形式的变性和再生形式的情况下。我们已经谨慎而热切地添加了新技能，仪器和试剂，以更好地满足用户的需求。在1995年，在儿童医院的慷慨帮助下，我们购买了一个左旋聚焦显微镜。 微型操纵器/微型注射器和舞台安装的环境室，因此允许 在生理条件下长期观察和操纵活细胞。通过这种方式，我们提供了8色能力荧光成像。我们的机构为该设备支付了费用，我们使用IDDRC资金部分支持技术人员。 我们对核心曲目进行了其他几个值得注意的技术添加，包括部分和整个胚胎的原位杂交。我们的通用目的不仅是为了使技术提供，而且是促进该方法实施以及数据解释的协商sen/ICE。当我们添加视频增强显微镜时，我们遵守了这一政策，以便更好地支持IDDRC研究者分析细胞内CA2+和Na+以及线粒体潜力的估计值。