Cellular Imaging Core

细胞成像核心

基本信息

批准号：
8555581
负责人：
MARK C WILLINGHAM
金额：
$ 5.77万
依托单位：
WAKE FOREST UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-02-01 至 2017-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8555581
关键词：
Area Atomic Force Microscopy Basic Science Behavior Cancer Center Cells Cessation of life Collaborations Complement Complex Comprehensive Cancer Center Comprehensive Cancer Center of Wake Forest University Disadvantaged Discipline Electron Microscope Electron Microscopy Electrons Equipment Fees Fluorescence Microscopy Funding Future Growth Housing Image Individual Institution Knowledge Laboratories Life Light Light Microscope Location Malignant Neoplasms Microinjections Microscope Microscopic Microscopy Microtomy Mission Negative Staining Ownership Phase Preparation Proteins Research Research Infrastructure Research Personnel Resource Sharing Resources Scanning Services Specimen Structure Surface System Techniques Time Tissues Travel Universities cancer cell cellular imaging cost digital imaging experience fluorescence imaging forest instrument instrumentation laser capture microdissection light microscopy member operation programs research study spatial relationship tool transmission process

项目摘要

Mission/Purpose: The Cellular Imaging Shared Resource provides access for Cancer Center members to advanced microscopy instruments, technical support for specimen preparation and scientific guidance in planning and applying these advanced microscopy techniques. Assets: The facility provides a full-service preparative laboratory for both light and electron microscopic techniques. Electron microscopy includes both transmission and scanning instruments, and the Shared Resource supports thin sectioning, surface sputter coating, critical point drying, negative staining and specimen embedding services. In addition, the Shared Resource supports routine microtomy and cryomicrotomy for both light and electron microscopy. Of the light microscopes housed in the Shared Resource, both inverted and upright formats are available for imaging with brightfield, phase contrast, Nomarski, dark field, and polarizing microscopy. Fluorescence microscopy using Zeiss and Olympus microscopes includes both widefield digital imaging in multiple spectra and confocal fluorescence imaging. Also, inverted microscopes equipped for time-lapse live cell experiments, single cell microinjection, and laser capture microdissection are available. A recent addition to Cellular Imaging is atomic force microscopy. This equipment has been purchased with assistance from the Comprehensive Cancer Center and further strengthens the scientific collaborations with Wake Forest physicists immensely. Usage: The Shared Resource gives priority to Cancer Center members for equipment access and operates a chargeback system that provides a 50% subsidy for membership. Cancer Center members represent approximately 50% or more of the Shared Resource's use. In the most recent year, 32 laboratories of Cancer Center members have utilized this Shared Resource. The access to advanced equipment is complemented by the expert guidance of Dr. Mark Willingham, Director of the Shared Resource. He, along with other technical experts on the Shared Resource support staff, assists investigators in the use of instruments and in planning of experiments using these instruments. Future Directions: This Shared Resource has recently added a new FEI transmission electron microscope to its instruments. In the near future, upgrades to a confocal system with

使命/目的：细胞成像共享资源为癌症中心成员提供访问权限先进的显微仪器、标本制备的技术支持和科学指导规划和应用这些先进的显微镜技术。资产：该设施为光学和电子显微镜提供全方位服务的制备实验室技术。电子显微镜包括透射和扫描仪器，以及共享的资源支持薄切片、表面溅射镀膜、临界点干燥、负染色和标本包埋服务。此外，共享资源支持常规切片术和用于光学和电子显微镜的冷冻切片术。共享空间内的光学显微镜资源，倒置和正置格式均可用于明场、相差、诺马斯基、暗视野和偏光显微镜。使用蔡司和奥林巴斯的荧光显微镜显微镜包括多光谱宽场数字成像和共焦荧光成像。此外，还配备了用于延时活细胞实验、单细胞显微注射和激光捕获的倒置显微镜可以进行显微解剖。细胞成像的最新成员是原子力显微镜。这在综合癌症中心的帮助下购买了设备，并进一步极大地加强了与维克森林物理学家的科学合作。用途：共享资源优先为癌症中心成员提供设备访问权，并运营退款系统为会员提供50%的补贴。癌症中心成员代表大约 50% 或更多的共享资源使用量。最近一年，32 个癌症实验室中心成员已使用此共享资源。先进设备的获取得到补充由共享资源总监 Mark Willingham 博士的专家指导。他与其他人一起共享资源支持人员中的技术专家协助调查人员使用仪器和使用这些仪器的实验计划。未来方向：此共享资源最近添加了新的 FEI 透射电子显微镜到它的仪器。在不久的将来，升级到共焦系统