Cellular Imaging Core

细胞成像核心

基本信息

批准号：
8016101
负责人：
MARK C WILLINGHAM
金额：
$ 7.5万
依托单位：
WAKE FOREST UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8016101
关键词：
Animals Basic Science Biochemical Cancer Center Cell Culture Techniques Cell Death Cell Separation Cells Clinical Comet Assay Comprehensive Cancer Center of Wake Forest University Confocal Microscopy Consultations Core Facility DNA Damage Development Electron Microscopy Equipment Fluorescence Fluorescence Microscopy Histology Housing Image Imagery Immune Individual Investigation Knowledge Laboratories Lasers Leukocytes Life Malignant - descriptor Malignant Neoplasms Methods Microdissection Microinjections Microscope Microscopy Mutation Normal Cell Pathway interactions Peer Review Phase Preparation Proteins Research Research Personnel Resources Scanning Services Staining method Suggestion System Technical Expertise Techniques Technology Virus cell determination cell growth cellular imaging digital digital video recording experience fluorescence microscope instrument killings meetings member neoplastic cell new technology programs success time use tool transmission process

项目摘要

The Cellular Imaging Core supplies access to equipment, technical support, and scientific consultation for Cancer Center researchers. The Core exists to meet the growing needs of CCCWFU researchers for sophisticated microscopy in conducting cancer-related research. One of the value-added functions of this Core is to establish and develop technologies prior to their availability in individual departments or laboratories and then, when practical, to facilitate the ability of departments or labs to develop their own capabilities, making room for new technologies in the Cancer Center core. From July 2004 through June 2005, the Core was used by 36 individual cancer researchers from five basic science and seven clinical departments. The facility maintains a full-service preparative laboratory and houses standard wide field fluorescence microscopes, transmission EM, scanning EM, laser scanning confocal (set up to support both standard confocal microscopy and the newer multiphoton excitation imaging), upright and inverted microscopes equipped for video and digital imaging using both phase contrast and fluorescence, as well as single cell microinjection, a macrodissection (stereo dissecting) microscope equipped with fluorescence excitation for GFP visualization in living animals, and a laser microdissection microscope. With the increased variety and sophistication of microscopes and the refinement of cellular staining methods has come a greater need for specialized knowledge in this field to correctly utilize the tools and efficiently advice novice users on which technique is most appropriate to their research. This type of technical expertise is best supported within the context of a core facility providing not only access to equipment, but critical technical and scientific consultation as well. The Core Director, Dr. Mark Willingham, is routinely immediately available to look at preparations, help with interpretations, and make suggestions for technique improvements. Recent new additions to the Core Laboratory support confocal and multiphoton microscopy, upright and inverted digital and video widefield microscopy (including long-term live cell video) with phase contrast and fluorescence, and laser microdissection microscopy. Of primary importance, however, is the experience of the director and staff in providing guidance for individual experimental approaches using these instruments, and that is a major focus of the core activity.

细胞成像核心提供设备、技术支持和科学咨询对于癌症中心的研究人员。核心的存在是为了满足 CCCWFU 研究人员日益增长的需求进行癌症相关研究的复杂显微镜。本产品的增值功能之一核心是在技术在各个部门或部门可用之前建立和开发技术实验室，然后在可行时促进各部门或实验室开发自己的能力能力，为癌症中心核心新技术腾出空间。从 2004 年 7 月到 6 月 2005 年，来自 5 个基础科学领域和 7 个临床领域的 36 名癌症研究人员使用了 Core 部门。该设施拥有一个提供全方位服务的制备实验室，并拥有标准的宽视野荧光显微镜、透射电镜、扫描电镜、激光扫描共焦（设置支持两种标准共焦显微镜和较新的多光子激发成像）、正置和倒置显微镜配备使用相衬和荧光以及单细胞的视频和数字成像显微注射，一种配备荧光激发的宏观解剖（立体解剖）显微镜活体动物中的 GFP 可视化和激光显微切割显微镜。随着显微镜的种类和复杂性的增加以及细胞染色的精细化方法越来越需要该领域的专业知识来正确使用工具和有效地建议新手用户哪种技术最适合他们的研究。这类技术专业知识在核心设施范围内得到最好的支持，不仅提供获得设备，但也有关键的技术和科学咨询。核心主任 Mark Willingham 博士，通常可以立即查看准备情况、帮助解释并提出建议技术改进。核心实验室最近新增的支持共焦和多光子显微镜、正置和倒置数字和视频宽场显微镜（包括长期活细胞视频），具有相差和荧光显微镜和激光显微切割显微镜。然而，最重要的是经验主任和工作人员为使用这些仪器的个人实验方法提供指导，这是核心活动的重点。