Transmission Electron Microscope for a Shared Core Facility

共享核心设施的透射电子显微镜

• 批准号: 7792690
• 负责人: MARK C WILLINGHAM
• 金额: $ 49.99万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792690
• 关键词: Applications Grants; Arts; Biological; Computers; Core Facility; Cryoultramicrotomy; Electron Microscope; Fluorescence; Funding; Grant; Image; Microscope; Microscopy; Microtomy; Negative Staining; Operating System; Request for Applications; Research Personnel; Research Project Grants; Research Support; Resolution; Sampling; Services; Shadowing (Histology); Specimen; Techniques; Time; Twin Multiple Birth; United States National Institutes of Health; Universities; aged; base; digital; digital imaging; experience; flexibility; forest; improved; instrument; laser capture microdissection; medical schools; tool; transmission process; voltage

DESCRIPTION (provided by applicant): This grant application requests funds for the purchase of an FEI Technai G2 Spirit Bio- Twin transmission electron microscope. This essential instrument is needed to replace an aged and undependable >30 year old Philips 400 TEM in the sole shared-use microscopy core facility in the Wake Forest University School of Medicine. This new instrument has computer-controlled operating systems, a digital imaging camera, and up to 120kV accelerating voltage to allow high resolution TEM imaging of biological specimens. Space, administration, highly-experienced technicians and institutional support for this shared microscopy core have been in place for over 20 years, and support the research of several NIH grant-funded investigators in many different departments, including a group of 10 investigators listed in this application as current major users. The core facility includes, in addition to the TEM instrument, an existing SEM instrument and light-based microscopy instruments, including digital fluorescence, confocal, time-lapse and laser-capture microdissection microscopes, all of which serve as strong correlative tools for the analysis of TEM samples. The core provides TEM support preparative services, including embedding and thin sectioning, cryoultramicrotomy, fixed angle and rotary shadowing and negative staining, as well as immunocytochemical techniques. The acquisition of this new microscope is critical to provide a state-of-the art instrument to continue the support of microscopy needs for NIH-funded investigators, with improved flexibility and dependability for these and for new research projects.

描述（由申请人提供）：本拨款申请要求提供资金购买 FEI Technai G2 Spirit Bio-Twin 透射电子显微镜。在维克森林大学医学院唯一共享的显微镜核心设施中，需要使用这一重要仪器来取代老化且不可靠的超过 30 年的飞利浦 400 TEM。这种新仪器具有计算机控制的操作系统、数码相机和高达 120kV 的加速电压，可对生物样本进行高分辨率 TEM 成像。对该共享显微镜核心的空间、管理、经验丰富的技术人员和机构支持已经到位 20 多年，并支持许多不同部门的几位 NIH 拨款资助的研究人员的研究，包括本报告中列出的由 10 名研究人员组成的小组作为当前主要用户的应用程序。除 TEM 仪器外，核心设施还包括现有的 SEM 仪器和光学显微镜仪器，包括数字荧光、共焦、延时和激光捕获显微解剖显微镜，所有这些仪器都是分析的强大关联工具TEM 样品。该核心提供 TEM 支持准备服务，包括包埋和薄切片、冷冻超薄切片、固定角度和旋转阴影和负染色，以及免疫细胞化学技术。购买这款新型显微镜对于提供最先进的仪器至关重要，以继续支持 NIH 资助的研究人员的显微镜需求，并提高这些研究人员和新研究项目的灵活性和可靠性。