NMR Metabolomics Core/SNYDER

NMR 代谢组学核心/SNYDER

基本信息

批准号：
8534960
负责人：
SUSAN J SUMNER
金额：
$ 23.36万
依托单位：
RESEARCH TRIANGLE INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-04 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8534960
关键词：
Adopted Age Biochemical Pathway Biochemistry Biological Biological Markers Biomedical Research Brain Cell Extracts Cells Client Code Collaborations Complement Consult Consultations Contracts Data Data Analyses Data Files Databases Deposition Detection Discriminant Analysis Disease Dose Ensure Ethnic Origin Functional disorder Funding Gender Generations Grant Health Housing Human Subject Research Instruction Internships Intervention Investigation Kidney Least-Squares Analysis Libraries Link Liquid substance Liver Mann-Whitney U Test Maps Mass Spectrum Analysis Measures Methods Molecular Weight NMR Spectroscopy Nuclear Magnetic Resonance Online Systems Outcome Pathway interactions Pattern Phenotype Plasma Preparation Principal Component Analysis Process Publications Quality Control Race Reproducibility Research Design Research Institute Research Personnel Resources Rodent Model Sampling Scientist Serum Signal Transduction Stream Structure Testing Testis Tissue Extracts Tissues Training Urine Uterus Work base cost cost effective data sharing experience insight meetings member metabolomics novel strategies nutrition outreach programs response

项目摘要

The NMR Metabolomics Core will be responsible for the generation and analysis of metabolomic profiles of biological fluids and tissue extracts. This Core will support the RTI RCMRC by providing standardized analysis and data in a consistent and coherent format, and providing expertise for interpreting results in the context of the study design and other phenotypic measures and data streams. Studies within the RTI Metabolomics Program have included the analysis of samples (e.g., urine, serum, extracts of serum, plasma, liver, kidney, brain, uterus, testes) from dose-response studies in rodent models to provide mechanistic insights and to develop biomarkers that are predictive of adverse health outcomes. The NMR Metabolomics Core also has experience evaluating samples from human subject research aimed at examining the responsivity to treatment and disease endpoints. The NMR Metabolomics Core is supported by our partners from the David H. Murdock Research Institute, which houses state-of-the-art 700 MHz and 950 MHz NMR spectrometers. Three internationally recognized metabolomics scientists (Dr. Jeremy Nicholson, Dr. Elaine Holmes, and Dr. Ian Wilson) will support the RTI RCMRC though consultation and collaboration. This core will ensure that the most cost-effective approach is used by conducting analysis of samples in appropriate size batches to reduce costs. Quality control features will include the use of internal standards, prior determination of reproducibility of methods with repeat measures, using single sample code throughout all aspects of center activities, and appropriate audits. The Core members will work with the RTI RCMRC Management Team to develop consistent formats and acceptable formats to deposit raw data or processed data files to the Data Repository and Coordinating Center according to the data sharing plan and requirements and the EC. The NMR Core will develop and adopt new methods as deemed appropriate by the EC. Members of the NMR Core will work with the Promotion and Outreach Core to develop materials for dissemination at meetings, and to provide web-based and internship training. The NMR Core will support the Mass Spectrometry Cores through metabolite identification.

NMR 代谢组学核心将负责生成和分析生物体液和组织提取物的代谢组学特征。该核心将通过以一致和连贯的格式提供标准化分析和数据，并提供在研究设计和其他表型测量和数据流背景下解释结果的专业知识来支持 RTI RCMRC。 RTI 内的研究代谢组学计划包括对啮齿动物模型剂量反应研究中的样本（例如尿液、血清、血清提取物、血浆、肝脏、肾脏、大脑、子宫、睾丸）进行分析，以提供机制见解并开发预测性生物标志物的不良健康后果。 NMR 代谢组学核心还具有评估人类受试者研究样本的经验，旨在检查对治疗和疾病终点的反应。 NMR 代谢组学核心由 David H. Murdock 研究所的合作伙伴提供支持，该研究所拥有最先进的 700 MHz 和 950 MHz NMR 波谱仪。三位国际知名的代谢组学科学家（Jeremy Nicholson 博士、Elaine Holmes 博士和 Ian Wilson 博士）将通过咨询和合作支持 RTI RCMRC。这个核心将确保采用最具成本效益的方法，对适当大小批次的样品进行分析，以降低成本。质量控制功能将包括使用内部标准、通过重复测量预先确定方法的再现性、在中心活动的所有方面使用单一样本代码以及适当的审核。核心成员将与 RTI RCMRC 管理团队合作，根据数据共享计划和要求以及 EC，开发一致的格式和可接受的格式，以将原始数据或处理后的数据文件存入数据存储库和协调中心。 NMR Core 将开发并采用 EC 认为合适的新方法。 NMR 核心成员将与推广和外展核心合作，开发在会议上传播的材料，并提供基于网络的实习培训。 NMR 核心将通过代谢物鉴定支持质谱核心。