LC/MS Metabolomics Core/THOMAS

LC/MS 代谢组学核心/THOMAS

• 批准号: 8534955
• 负责人: SUSAN J SUMNER
• 金额: $ 115.49万
• 依托单位: RESEARCH TRIANGLE INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-04 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8534955
• 关键词: Biochemical; Biological; Cells; Client; Commit; Communities; Complement; Consult; Contracts; Coupled; Data; Databases; Ensure; Genomics; Health; Human; Liquid substance; Mass Spectrum Analysis; Methodology; Methods; Mission; Molecular Weight; Pharmacological Treatment; Phase; Physiological; Proteomics; Research; Research Infrastructure; Research Institute; Research Personnel; Resources; Serum; Services; System; Technology; Time; Tissues; Urine; Work; analytical method; base; data integration; design; expectation; experience; improved; instrumentation; liquid chromatography mass spectrometry; meetings; metabolomics; method development; outreach; stable isotope; statistics

The study of the the low-molecular weight complement of cells, tissues, and biological fluids can be useful in assessing human health or the biochemical effects of exposures or pharmacological treatments on physiological systems. RTI is able to provide a well-established and uniquely broad range of experience and expertise with LC/MS-based analytical methods that are widely used in metabolomics research. With Dr. Jeremey Nicholson, Dr. Elaine Holmes, and Dr. Ian Wilson serving as co-investigators and consultants to the RCMRC, the proposed LC/MS-based metabolomics technology core will ensure that clients have access to the most recent methods available for data acquaition, analysis, and interpretation. Hence, the specific aims of the proposed LC/MS core are designed to leverage these critical capabilities to meet and exceed the requirements of the RCMRC and the service expectations of its scientific researchers. By integrating these capabilities and expertise with other cores, the LC/MS core at RTI will provide (1) consulting and educational outreach services; (2) the analytical capacity for the conduct of standardized LC/MS-based metabolomic methods and the development and inclusion of new methods and technology to meet the needs of ongoing and planned metabolomics studies, and (3) raw data and summary statistics in a manner consistent with the requirements ofthe DRCC and the needs ofthe client. Our LC/MS broad-spectrum metabolomics approach is designed to look at nonpolar and polar analytes using reverse phase and normal phase (HILIC) chromatographic methods coupled to time-of-flight mass spectrometry. Targeted metabolomic studies are supported on triple quadrupoles or Q-trap platforms with chromatographic conditions optimized for the target group of metabolites, and employ stable-isotope-dilution whenever possible. RTI is committed to providing a sustained infrastructure of staff, facilities, and instrumentation to support these efforts, and the RCMRC intends to continuously integrate the broad-spectrum metabolomics data with metabolite identification and peak matching data to generate a comprehensive reference database, and make it available as a resource to the metabolomics research community.

对细胞、组织和生物液体的低分子量补体的研究可用于评估人类健康或暴露或药物治疗对生理系统的生化影响。 RTI 能够通过广泛应用于代谢组学研究的基于 LC/MS 的分析方法提供完善且独特的广泛经验和专业知识。和 Jeremey Nicholson 博士、Elaine Holmes 博士和 Ian Wilson 博士作为 RCMRC 的联合研究员和顾问，拟议的基于 LC/MS 的代谢组学技术核心将确保客户能够获得最新的数据方法获取、分析和解释。因此，具体 拟议的 LC/MS 核心的目标是利用这些关键功能来满足并超越 RCMRC 的要求及其科学研究人员的服务期望。通过将这些能力和专业知识与其他核心相集成，RTI 的 LC/MS 核心将提供 (1) 咨询和教育推广服务； (2) 进行基于 LC/MS 的标准化代谢组学的分析能力 方法以及新方法和技术的开发和纳入，以满足正在进行和计划的代谢组学研究的需求，以及 (3) 以符合 DRCC 要求和客户需求的方式提供原始数据和汇总统计数据。我们的 LC/MS 广谱代谢组学方法旨在使用反相和正相 (HILIC) 观察非极性和极性分析物 色谱方法与飞行时间质谱联用。靶向代谢组学研究由三重四极杆或 Q-trap 平台支持，其色谱条件针对目标代谢物组进行了优化，并尽可能采用稳定同位素稀释。 RTI 致力于提供持续的人员、设施和仪器基础设施来支持这些努力，RCMRC 打算不断将广谱代谢组学数据与代谢物鉴定和峰匹配数据相整合，以生成综合参考数据库，并使其成为可能。作为代谢组学研究界的资源。